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YAVUZ, AYŞE NUR

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Eczacılık Fakültesi

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YAVUZ

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AYŞE NUR

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Open Access
A novel treatment strategy for preterm birth: Intra-vaginal progesterone-loaded fibrous patches
(ELSEVIER, 2020-10) YAVUZ, AYŞE NUR; Cam, Muhammet Emin; Hazar-Yavuz, Ayse Nur; Cesur, Sumeyye; Ozkan, Ozan; Alenezi, Hussain; Sasmazel, Hilal Turkoglu; Eroglu, Mehmet Sayip; Brako, Francis; Ahmed, Jubair; Kabasakal, Levent; Ren, Guogang; Gunduz, Oguzhan; Edirisinghe, Mohan
Progesterone-loaded poly(lactic) acid fibrous polymeric patches were produced using electrospinning and pressurized gyration for infra-vaginal application to prevent preterm birth. The patches were intravaginally inserted into rats in the final week of their pregnancy, equivalent to the third trimester of human pregnancy. Maintenance tocolysis with progesterone-loaded patches was elucidated by recording the contractile response of uterine smooth muscle to noradrenaline in pregnant rats. Both progesterone-loaded patches indicated similar results from release and thermal studies, however, patches obtained by electrospinning had smaller average diameters and more uniform dispersion compared to pressurized gyration. Patches obtained by pressurized gyration had better results in production yield and tensile strength than electrospinning; thereby pressurized gyration is better suited for scaled-up production. The patches did not affect cell attachment, viability, and proliferation on Vero cells negatively. Consequently, progesterone-loaded patches are a novel and successful treatment strategy for preventing preterm birth.
Open Access
Effects of Cornus mas L. on lipid peroxidation and anti-oxidative enzyme activity in high fat diet fed rats
(2023-02-01) KARĞIN, DİCLE; YAVUZ, AYŞE NUR; ÇAM, MUHAMMET EMİN; AKTAÇ, ŞULE; Karğın D., Aktaç Ş., Yavuz A. N., Çam M. E.
Cornelian cherry (Cornus mas L.) has been used for centuries as a traditional herbal medicine in Europe and Asia. In this study, we aimed to describe the effect of Cornus mas L. (C. mas) on the activity of the antioxidant enzymes and a detoxification agent in rats fed a high-fat diet. Forty-eight adult Sprague Dawley rats were randomly assigned to six groups of eight animals each: Standard diet (Control), High Fat Diet (HFD), HFD + C. mas (200 mg/kg/day; 8 weeks), HFD + Atorvastatin (20 mg/kg/day; 8 weeks), HFD post-treated with C. mas (200 mg/kg/day; 4 weeks), HFD posttreated with Atorvastatin (20 mg/kg/day; 4 weeks). The activity of the antioxidant enzymes, Superoxide dismutase (SOD), Catalase (CAT), Glutathione peroxidase (GPx), detoxification agent glutathione (GSH), and oxidative stress parameter thiobarbituric acid reactive substances (TBARS) were measured in the liver tissues. GPx, SOD, and CAT enzyme levels were decreased in HFD groups compared to the control (p < 0.05). However, Cornus mas L. promoted antioxidant activity by increasing GPx, SOD, and CAT enzymes and It also reduced oxidative stress (as an increase in GSH) both in the HFD + C. mas group and the HFD post-treated C. mas group compared to the HFD group (p < 0.05). Our study showed that feeding a high-fat diet increases oxidative stress. Cornus mas L treatment improves antioxidant enzyme activity and oxidative stress parameters in the liver tissues of rats.
Open Access
A Comparison Study of Fiber Diameter's Effect on Characteristic Features of Donepezil/Curcumin-Loaded Polycaprolactone/Polylactic Acid Nanofibers
(2022-05-01) EKENTOK ATICI, CEYDA; GÜNDÜZ, OĞUZHAN; ÇAM, MUHAMMET EMİN; TATAR, ESRA; YAVUZ, AYŞE NUR; Aydin S., Kabaoglu I., Guler E., Topal F., YAVUZ A. N., EKENTOK ATICI C., TATAR E., Gurbuz F., GÜNDÜZ O., ÇAM M. E.
Nanofibers (NFs) offer an alternative option for the treatment of Alzheimer\"s disease (AD) by addressing unmet clinical problems. In this study, anti-AD drugs, donepezil (DO) and curcumin (CUR), are loaded in polylactic acid/polycaprolactone NFs. The effect of fiber diameter on drug release behavior is mainly observed, and the successful loading of DO and CUR to NFs is demonstrated. The tensile strength of DO/CUR-loaded NFs (DNFs) with lower fiber diameter is found to be higher. The working temperature is increased by the decrease of glass transition temperature and increase of the melting temperature after loading drugs. Furthermore, the increase in the percentage of swelling and decrease in the degradation rate for NFs are observed due to the increase of fiber diameter. Encapsulation efficiency and burst release percentages for DNFs are augmented by the increase of fiber diameter. Nevertheless, DNFs exhibit a sustained drug release manner over 2 weeks. NFs do not demonstrate a toxic effect on L929 (mouse fibroblast) cells, and additionally, they promote cell proliferation. Considering all these results, it is proven that the fiber diameter affects all characteristic features of NFs, and DNFs lead to a new and promising drug delivery system for the treatment of AD.
Open Access
Sodium-glucose co-transporter inhibitor dapagliflozin attenuates cognitive deficits in sporadic Alzheimer’s rat model
(2022-01-01) KABASAKAL, LEVENT; YAVUZ, AYŞE NUR; YAVUZ A. N., Yildiz S., Kaya R. K., ÇAM M. E., KABASAKAL L.
© 2022 Marmara University Press.Alzheimer’s disease (AD) and Type 2 Diabetes Mellitus (T2DM) are both characterized by similar pathologies, and studies have shown that various drugs from both groups may be effective in another. The effects of sodium-glucose co-transporter (SGLT)2 inhibitors in AD are unknown. According to molecular docking studies, various SGLT inhibitors have acetylcholinesterase (AChE) inhibition activity, which is therapeutic target for AD. In this study, we investigated the effects of SGLT2 inhibitor dapagliflozin on intracerebroventricular (icv) streptozotocin (STZ) induced sporadic AD rats using open field test (OFT), novel object recognition test (NORT), passive avoidance test (PAT) and Morris’s water maze test (MWMT). Rats were randomly divided into 4 groups: vehicle-control, icv STZ, dapagliflozin, and galantamine treatments groups. STZ was injected bilaterally in two divided doses on day 1 and 3. All treatments began on day 1 and continued to day 21. OFT was performed for evaluating animal locomotor activity and anxiety. Other behavioral tests, NORT, PAT, and MWMT was performed for determining of learning and memory ability of rats. On day 21, all rats were decapitated. Our results showed that treatments with dapagliflozin and galantamine significantly prevented learning and memory deficits in behavioral tests. Dapagliflozin may present as a potent dual inhibitor of SGLT2 and AChE. Our results may form the basis of future dual treatment against diabetes and diabetes-related neurological diseases. The effects of possible dual AChE and SGLT2 inhibition by a single compound may help to establish new drugs that perform both anti-AD and antidiabetic action.
Open Access
The methanolic extracts of Teucrium polium L. and Micromeria fruticosa (L.) Druce subsp. brachycalyx P. H. Davis improve diabetes in streptozotocin/nicotinamide-induced type 2 diabetic female Sprague Dawley rats
(2023-12-01) ÇAM, MUHAMMET EMİN; YAVUZ, AYŞE NUR; TAŞKIN, TURGUT; KABASAKAL, LEVENT; ELÇİOĞLU, HATİCE KÜBRA; Sermet S., ÇAM M. E., YAVUZ A. N., TAŞKIN T., Kabatas G. S., YAZIR Y., KABASAKAL L., ELÇİOĞLU H. K.
Background: Teucrium polium and Micromeria fruticosa subspecies are known to be used in traditional medicine for diabetes treatment. Objective: The present study aimed to examine the potential antidiabetic effects and the mechanisms of antidiabetic actions of Teucrium polium L. methanolic extract (TP) and Micromeria fruticosa (L.) Druce subsp. brachycalyx P. H. Davis methanolic extract (MF) on rats with type 2 diabetes mellitus (DM). Methods: Streptozotocin (STZ) and nicotinamide (NA) were injected intraperitoneally to induce type 2 diabetes mellitus (T2DM) in rats. Plant extracts’ antidiabetic mechanisms of action were investigated with biochemical analyses and histopathological analyses performed. Results: TP and MF treatments reduced the blood glucose levels compared to the untreated diabetic rats. TP reduced TNF-α levels in serum, increased insulin levels in serum and pancreas, reduced SGLT-2 levels in kidneys, reduced GLUT-2 levels in the ileum; and MF reduced TNF-α levels, and increased insulin levels in serum and pancreas, increased GLP-1 levels, and reduced GLUT-2 levels in the ileum, and reduced SGLT-2 levels in kidneys. Treatments improved the histopathological results in the pancreas, kidney, and liver. Conclusions: The results presented in this study demonstrated that TP and MF both have potential antidiabetic effects and may be effective in T2DM treatment.
Open Access
Rosa canina L. improves learning and memory-associated cognitive impairment by regulating glucose levels and reducing hippocampal insulin resistance in high-fat diet/streptozotocin-induced diabetic rats
(2023-09-15) ERTAŞ, BÜŞRA; YAVUZ, AYŞE NUR; TAŞKIN, TURGUT; ÇAM, MUHAMMET EMİN; ERTAŞ B., YAVUZ A. N., Topal F., Keles-Kaya R., Karakus Ö., Ozcan G. S., TAŞKIN T., ÇAM M. E.
Ethnopharmacological relevance: Recent studies claim that Type-2 diabetes mellitus (T2DM) and Alzheimer\"s disease (AD) overlap in several common pathological pathways which from neuronal damage to impaired memory performance. It is known that the use of Rosa canina L. (R. canina) as medicine in folk medicine dates back to ancient times and is used in the treatment of nervous diseases in Persian medicine. However, the effect of R. canina on diabetes-related cognitive decline and memory impairment has not yet been studied. Aim of the study: We evaluated the impact of T2DM on AD-like alterations and examined the molecular mechanism of a possible effect of R. canina on cognitive alterations in diabetic rats. Materials&methods: R. canina ethanol extract was obtained by maceration method. This study was performed with male Sprague-Dawley rats fed with a high-fat diet (HFD) for 8 weeks, low-dose streptozotocin (STZ; 35 mg/kg IP) injection for 4 weeks, and R. canina (250 mg/kg; per oral) and metformin (400 mg/kg; per oral) administration for 4 weeks. The weight and blood glucose of rats were measured weekly. To evaluate glucose tolerance area under the curve (AUC) was calculated by performing an oral glucose tolerance test. Then the rats were subjected to behavioural tests, and their hippocampus and cortex tissues were obtained for biochemical and morphological analyses. Results: R. canina could manage glucose responsiveness by reducing post-prandial blood glucose levels, preventing weight loss, and raising serum insulin levels in T2DM-induced rats. Behavioural tests showed that R. canina significantly improves diabetes-related cognitive decline in recall and long-term memory. Treatment with R. canina significantly reversed HFD/STZ-induced increases in insulin, amyloid-β, amyloid precursor protein levels, and acetylcholinesterase activity in the prefrontal cortex and hippocampus. Furthermore, histological analyzes revealed the protection of R. canina against neuronal disruption in the cortical and hippocampal CA3 region caused by chronic hyperglycemia. Conclusion: Analyzed collectively, these results suggest that R. canina can correct T2DM-related cognitive decline may be attributed to insulin pathway modulation, prevention of amyloid deposition, and increased cholinergic transmission.
Open Access
Accelerated diabetic wound healing by topical application of combination oral antidiabetic agents-loaded nanofibrous scaffolds: An in vitro and in vivo evaluation study
(ELSEVIER, 2021-02) YAVUZ, AYŞE NUR; Cam, Muhammet Emin; Ertas, Busra; Alenezi, Hussain; Hazar-Yavuz, Ayse Nur; Cesur, Sumeyye; Ozcan, Gul Sinemcan; Ekentok, Ceyda; Guler, Ece; Katsakouli, Christina; Demirbas, Zehra; Akakin, Dilek; Eroglu, Mehmet Sayip; Kabasakal, Levent; Gunduz, Oguzhan; Edirisinghe, Mohan
The combination of oral antidiabetic drugs, pioglitazone, metformin, and glibenclamide, which also exhibit the strongest anti-inflammatory action among oral antidiabetic drugs, were loaded into chitosan/gelatin/polycaprolactone (PCL) by electrospinning and polyvinyl pyrrolidone (PVP)/PCL composite nanofibrous scaffolds by pressurized gyration to compare the diabetic wound healing effect. The combination therapies significantly accelerated diabetic wound healing in type-1 diabetic rats and organized densely packed collagen fibers in the dermis, it also showed better regeneration of the dermis and epidermis than single drug-loaded scaffolds with less inflammatory cell infiltration and edema. The formation of the hair follicles started in 14 days only in the combination therapy and lower proinflammatory cytokine levels were observed compared to single drug-loaded treatment groups. The combination therapy increased the wettability and hydrophilicity of scaffolds, demonstrated sustained drug release over 14 days, has high tensile strength and suitable cytocompatibility on L929 (mouse fibroblast) cell and created a suitable area for the proliferation of fibroblast cells. Consequently, the application of metformin and pioglitazone-loaded chitosan/gelatin/PCL nanofibrous scaffolds to a diabetic wound area offer high bioavailability, fewer systemic side effects, and reduced frequency of dosage and amount of drug.
Open Access
The comparision of glybenclamide and metformin-loaded bacterial cellulose/gelatin nanofibres produced by a portable electrohydrodynamic gun for diabetic wound healing
(PERGAMON-ELSEVIER SCIENCE LTD, 2020-07) YAVUZ, AYŞE NUR; Cam, Muhammet Emin; Crabbe-Mann, Maryam; Alenezi, Hussain; Hazar-Yavuz, Ayse Nur; Ertas, Busra; Ekentok, Ceyda; Ozcan, Gul Sinemcan; Topal, Fadime; Guler, Ece; Yazir, Yusufhan; Parhizkar, Maryam; Edirisinghe, Mohan
Wound dressings made from natural polymers are an important aspect of biomaterials. Protein-based materials are less likely to instigate an immunogenic response and have the capacity to degrade in vivo, also without triggering an inflammatory response. Therefore, gelatin (GEL) was chosen and combined with bacterial cellulose (BC) to produce nanofibres and the potential of an all-natural polymer construct was determined. GEL and BC were successfully electrospun with metformin (Met) and glybenclamide (Gb) using a portable, point of need electrospinning set up. The virgin fibre group exhibited a significant effect on the proliferation of L929 (mouse fibroblast) cells but all fibre samples can safely be applied on wound site without risk of cytotoxicity. According to the results obtained by animal tests, the GEL-BC-Gb group showed better recovery than the GEL-BC-Met group. Diabetic wounds treated with GEL-BC-Met were characterized by moderate re-epithelialization and partially organized granulation tissue. Moderate to complete re-epithelialization and well-formed granulation tissue were observed in diabetic wounds treated with GEL-BC-Gb. The histologic scores obtained on day 14 confirmed that the GEL-BC-Gb group played a stronger wound-healing role compared to the GEL-BC-Met group. The highest decrease of TNF-alpha level was observed in the GEL-BC-Gb group at the end of the experiment but there is no significant difference between drug-loaded fibre groups. Therefore, topical administration of ma and Gb in a sustained release form has a high potential for diabetic wound healing with high bioavailability and fewer systemic side effects but Gb showed better improvement according to the results of the animal tests.