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YALÇIN, AHMET SUHA

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YALÇIN

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AHMET SUHA

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1990 - 19953
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End date: 2009 × Subject: Humans ×

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Now showing 1 - 3 of 3
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    Interaction of 3H-defibrotide with cultured human umbilical vein endothelial cells
    (1990) YALÇIN, AHMET SUHA; Bilsel, S.; Yalçin, A. S.; Taga, Y.; Emerk, K.
    Defibrotide is a profibrinolytic and antithrombotic drug which seems to modulate endothelial cell function. In this study, a method for radioactive labeling of the drug and its interaction with cultured endothelial cells is proposed. 3H-Acetic anhydride was used to label defibrotide. Endothelial cells obtained by collagenase treatment of human umbilical cord veins were cultured in 24-welled plastic culture dishes. Binding experiments were carried out by incubating cell cultures with media containing various concentrations of labeled defibrotide. Our results showed that labeled defibrotide has a KL value of 4.2 micrograms/ml for endothelial cells. Although the presence of a specific transporter is possible, the high molecular weight of the fraction used suggests that the interaction is binding to a specific receptor.
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    Cumene hydroperoxide-induced chemiluminescence in human erythrocytes: effect of antioxidants and sulfhydryl compounds
    (1992) YALÇIN, AHMET SUHA; Yalçin, A. S.; Sabuncu, N.; Emerk, K.
    1. The time-course of cumene hydroperoxide-induced changes in lipid peroxidation, protein sulfhydryl groups and chemiluminescence intensity was determined in human erythrocytes. 2. Increase in lipid peroxidation was maximal within 60 min of incubation and was paralleled by a decrease in protein sulfhydryl groups and an increase in chemiluminescence formation. 3. A standard assay system was established to investigate the protective effects of antioxidants and scavenger compounds on cumene hydroperoxide-induced chemiluminescence formation. 4. Chain-breaking antioxidants (i.e. butylated hydroxytoluene) and sulfhydryl compounds (i.e. dithiothreitol) were able to suppress chemiluminescence formation. 5. Our results suggested that secondary free radicals, as well as sulfhydryl groups of proteins are involved in cumene hydroperoxide-induced chemiluminescence formation.
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    Evaluation of oxidant stress in chronic hemodialysis patients: use of different parameters
    (1995) YALÇIN, AHMET SUHA; Haklar, G.; Yeğenağa, I.; Yalçin, A. S.
    Patients with chronic renal failure, particularly those undergoing regular dialysis treatment (RDT) are candidates for free radical damage. It is difficult to quantitate free radicals because of their short half-lives and reactive nature. Therefore, indirect methods measuring products of lipid peroxidation and protein oxidation are preferred. The present study displays a profile of lipid peroxidation and protein oxidation parameters, which are more sensitive and specific than the widely used method measuring thiobarbituric acid reactive substances (TBARS), adapted to the plasma and erythrocyte samples of RDT patients. We have observed increased levels of plasma and erythrocyte lipid peroxidation and also demonstrated increased protein oxidation in erythrocyte membranes of RDT patients.