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ŞEN, ALİ

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Author: DOĞAN, AHMET × Subject: antioxidant activity ×

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    Chemical composition of endemic Stachys subnuda Montbret & Aucher ex Benth. essential oil and its anti-inflammatory and antioxidant activities
    (TAYLOR & FRANCIS INC, 2019) DOĞAN, AHMET; Sen, Ali; Kurkcuoglu, Mine; Bitis, Leyla; Dogan, Ahmet; Baser, Kemal Husnu Can
    The essential oil of aerial parts of Stachys subnuda Montbret & Aucher ex Benth (S. subnuda) was analyzed by gas chromatography (GC) and GC/mass spectrometry (MS). Anti-inflammatory activity was evaluated by lipoxygenase inhibition assay. The antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azinobis-3-ethylbenzothiozoline-6-sulfonic acid (ABTS) assays. Sixty-two constituents, comprising 97.1% of the total essential oil composition, were identified. Phytol (25.2%), beta-caryophyllene (11.2%), germacrene D (6.9%), and hexadecanoic acid (6.9%) were the major compounds of aerial part essential oils of S. subnuda. The essential oil showed significant radical scavenging activity with 50% inhibition (IC50) values of 0.124 and 0.204 mg/mL for ABTS and DPPH radicals, respectively. The essential oil exhibited moderate anti-inflammatory activity with an IC50 value of 0.419 mg/mL. The results indicated that S. subnuda essential oil had good anti-inflammatory and antioxidant activity. Also, this is first study on chemical composition, anti-inflammatory and antioxidant activity of S. subnuda essential oil.
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    Two Acylated Isoscutellarein Glucosides with Anti-Inflammatory and Antioxidant Activities Isolated from Endemic Stachys Subnuda Montbret & Aucher ex Benth
    (SLOVENSKO KEMIJSKO DRUSTVO, 2019-12-18) DOĞAN, AHMET; Sen, Ali; Goger, Fatih; Dogan, Ahmet; Bitis, Leyla
    In this study, we report anti-inflammatory and antioxidant activities of two acylated isoscutellarein glucosides isolated from ethyl acetate extract of Stachys subnuda aerial part. 4'-O-methylisoscutellarein-7-O-2 ''-O-(6 ''' acetyl-beta-D-allopyranosyl)-beta-D-glucopyranoside (SS1) and isoscutellarein-7-O-2 ''-O-(6 '''-O-acetyl-beta-D allopyranosyl)-beta-D -glucopyranoside (SS2) were isolated as major compounds from ethyl acetate extract (SSEA). Also, 2 hydroxycinnamic acid derivatives, and 5 isoscutellarein glucoside derivatives in the SSEA were identified using LC-MS/MS. SS1 with IC50 values of 2.35 and 1.98 mu g/mL and SS2 with IC50 values 13.94 and 12.76 mu g/mL showed fairly strong antioxidant activity against DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS (2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid) radicals, respectively. SS1 and SS2 inhibited 5-lipoxygenase (5-LOX) activity with IC(50 )values of 47.23 and 41.60 mu g/mL, respectively. The results demonstrated that SS1 and SS2 have significant anti-inflammatory and antioxidant potential. Acylated flavonoid glycosides have been first reported for Stachys subnuda. Also, the activities of SS1 and SS2 have been investigated for the first time in this study.
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    Chemical composition and biological activities of endemicTripleurospermum conoclinium(Boiss. & Balansa) Hayek essential oils
    (WILEY, 2020) DOĞAN, AHMET; Servi, Huseyin; Sen, Ali; Dogan, Ahmet
    The volatile oils of flowers (TFE), leaves (TLE) and stems (TSE) ofTripleurospermum conoclinium(Boiss. & Balansa) Hayek were obtained by hydrodistillation method for 3 hours with Clevenger-type apparatus. Chemical composition of oils was determined by GC-MS analyses. Antidiabetic, anti-inflammatory and antioxidant activities of oils were tested by alpha-glucosidase inhibitory, 5-lipoxygenase inhibitory and DPPH/ABTS radical scavenging methods, respectively. Eighty-five constituents were identified in flower oil (78.3%). The major compounds of flower oil were artemisia ketone (10.5%), beta-oplopenone (6.0%), tricosane (4.5%), terpinen-4-ol (3.7%) and artemisia alcohol (3.5%). Sixty-two constituents were identified in leaf oil (67.0%). 1-octadecanol (11.5%), caryophyllene oxide (5.7%), elemol (3.9%), phytol (3.5%), ledene oxide (3.3%) and artemisia ketone (3.1%) were found as main constituents in the leaf oil. Forty-six constituents were determined in the stem oil (75.1%). The main compounds of the stem oil were 1-octadecanol (11.1%), beta-oplopenone (5.5%), ledene oxide (5.5%), caryophyllene oxide (4.4%) and phytol (3.0%). TLE and TSE displayed strong antidiabetic activity with IC(50)values of 21.30 and 29.57 mu g/mL against alpha-glucosidase enzyme, respectively. TLE showed the best anti-inflammatory activity with an IC(50)value of 91.02 mu g/mL while TFE and TSE exhibited moderate activity with IC(50)values of 186.50 and 257.10 mu g/mL, respectively. TFE and TLE were found to possess moderate antioxidant activity with IC(50)values of 572.80 and 385.80 mu g/mL against ABTS radical while all essential oils showed weak activity against DPPH radical. To the best of our knowledge, this is first report on chemical composition and biological activities ofTconocliniumessential oils.