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AKAKIN, DİLEK

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AKAKIN

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DİLEK

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2010 - 201912020 - 20231
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Author: ŞENER, GÖKSEL × Has files: true ×

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    PublicationOpen Access
    Cotinus coggygria scop. Attenuates acetic acid-induced colitis in rats by regulation of inflammatory mediators
    (2023-03-01) ŞEN, ALİ; ERTAŞ, BÜŞRA; YILDIRIM, AYBENİZ; AKAKIN, DİLEK; BİTİŞ, LEYLA; ŞENER, GÖKSEL; Şen A., Ertaş B., Çevik Ö., Yıldırım A., Gökçeoğlu-Kayalı D., Akakın D., Bitiş L., Şener G.
    In traditional medicine, many medicinal plants are used in the treatment of various diseases caused by infammation. The objective of the present study is to elucidate for the frst time the efects of Cotinus coggygria (CC) ethanol extract (CCE) on colonic structure and infammation of acetic acid-induced ulcerative colitis in rats. Colonic damage was assessed using disease activity index score, enzyme-linked immunosorbent assay, and hematoxylin–eosin staining. Also, in vitro antioxidant activity of CCE was investigated by ABTS methods. Total phytochemical content of CCE was measured spectroscopically. Acetic acid caused colonic damage according to disease activity index and macroscopic scoring. CCE signifcantly reversed these damages. While the levels of proinfammatory cytokines TNFalpha, IL-1beta, IL-6, and TGF-1beta increased in tissue with UC, IL-10 level decreased. CCE increased infammatory cytokine levels to values close to the sham group. At the same time, while markers indicating disease severity such as VEGF, COX-2, PGE2, and 8-OHdG indicated the disease in the colitis group, these values returned to normal with CCE. Histological research results support biochemical analysis. CCE exhibited signifcant antioxidant against ABTS radical. Also, CCE was found to have a high content of total polyphenolic compounds. These fndings provide evidence that CCE might be beneft as a promising novel therapy in the treatment of UC in humans due to high polyphenol content and justify the use of CC in folkloric medicine for treatment of infamed diseases.
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    PublicationOpen Access
    Neuroprotective Effects of Alpha-Lipoic Acid in Experimental Spinal Cord Injury in Rats
    (TAYLOR & FRANCIS LTD, 2010-01) VELİOĞLU ÖĞÜNÇ, AYLİZ; Toklu, Hale Z.; Hakan, Tayfun; Celik, Hasan; Biber, Necat; Erzik, Can; Ogunc, Ayliz V.; Akakin, Dilek; Cikler, Esra; Cetinel, Sule; Ersahin, Mehmet; Sener, Goksel
    Background: Oxidative stress is a mediator of secondary injury to the spinal cord following trauma. Objective: To investigate the putative neuroprotective effect of a-lipoic acid (LA), a powerful antioxidant, in a rat model of spinal cord injury (SCI). Methods: Wistar albino rats were divided as control, vehicle-treated SCI, and LA-treated SCI groups. To induce SCI, a standard weight-drop method that induced a moderately severe injury (100 g/cm force) at T10 was used. Injured animals were given either 50 mg/kg LA or saline at 30 minutes postinjury by intraperitoneal injection. At 7 days postinjury, neurologic examination was performed, and rats were decapitated. Spinal cord samples were taken for histologic examination or determination of malondialdehyde (MDA) and glutathione (GSH) levels, myeloperoxidase (MPO) activity, and DNA fragmentation. Formation of reactive oxygen species in spinal cord tissue samples was monitored by using a chemiluminescence (CL) technique. Results: SCI caused a significant decrease in spinal cord GSH content, which was accompanied with significant increases in luminol CL and MDA levels, MPO activity, and DNA damage. Furthermore, LA treatment reversed all these biochemical parameters as well as SO-induced histopathologic alterations. Conversely, impairment of the neurologic function caused by SCI remained unchanged. Conclusion: The present study suggests that LA reduces SCI-induced oxidative stress and exerts neuroprotection by inhibiting lipid peroxidation, glutathione depletion, and DNA fragmentation.