Person: TOKSOY ÖNER, EBRU
Loading...
Email Address
Birth Date
2 results
Search Results
Now showing 1 - 2 of 2
Publication Metadata only A novel levansucrase purified from halophilic bacteria halomonas smyrnensis AAD6T(2013-04-24) TURANLI, BESTE; ARĞA, KAZIM YALÇIN; TOKSOY ÖNER, EBRU; TURANLI B., ARĞA K. Y., TOKSOY ÖNER E.Publication Open Access Characterization of inulolytic enzymes from the Jerusalem artichoke-derived Glutamicibacter mishrai NJAU-1(2022-09-01) TOKSOY ÖNER, EBRU; Lian D., Zhuang S., Shui C., Zheng S., Ma Y., Sun Z., Porras-Dominguez J. R., TOKSOY ÖNER E., Liang M., Van den Ende W.The rhizosphere context of inulin-accumulating plants, such as Jerusalem artichoke (Helianthus tuberosus), is an ideal starting basis for the discovery of inulolytic enzymes with potential for bio fructose production. We isolated a Glutamicibacter mishrai NJAU-1 strain from this context, showing exo-inulinase activity, releasing fructose from fructans. The growth conditions (pH 9.0; 15 degrees C) were adjusted, and the production of inulinase by Glutamicibacter mishrai NJAU-1 increased by 90% (0.32 U/mL). Intriguingly, both levan and inulin, but not fructose and sucrose, induced the production of exo-inulinase activity. Two exo-inulinase genes (inu/ and inu2) were cloned and heterologously expressed in Pichia pastoris. While INU2 preferentially hydrolyzed longer inulins, the smallest fructan 1-kestose appeared as the preferred substrate for INU1, also efficiently degrading nystose and sucrose. Active site docking studies with GFn- and Fn-type small inulins (G is glucose, F is fructose, and n is the number of beta (2-1) bound fructose moieties) revealed subtle substrate differences between INU1 and INU2. A possible explanation about substrate specificity and INU\"s protein structure is then suggested.