Person: AKSU, MEHMET BURAK
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AKSU
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MEHMET BURAK
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Publication Metadata only Development of Satureja cuneifolia-loaded sodium alginate/polyethylene glycol scaffolds produced by 3D-printing technology as a diabetic wound dressing material(ELSEVIER, 2020) OKTAR, FAİK NÜZHET; Ilhan, Elif; Cesur, Sumeyye; Guler, Ece; Topal, Fadime; Albayrak, Deniz; Guncu, Mehmet Mucahit; Cam, Muhammet Emin; Taskin, Turgut; Sasmazel, Hilal Turkoglu; Aksu, Burak; Oktar, Faik Nuzhet; Gunduz, OguzhanAcute wounds are a common health problem, with millions of people affected and decreased granulation tissue formation and vascularization, it is also a big challenge for wound care researchers to promote acute wound healing around the globe. This study aims to produce and characterize Satureja cuneifolia plant extract (SC) blended with sodium alginate (SA) /polyethylene glycol (PEG) scaffolds for the potential treatment of diabetic ulcer. SA/PEG scaffolds were prepared by adding different concentrations (1, 3, and 5 wt%) of PEG to 9 wt% SA. The morphological and chemical composition of the resulting 3D printed composite scaffolds was determined using scanning electron microscopy (SEM) and Fourier transforms infrared spectroscopy (FTIR), respectively. Mechanical and thermal properties, swelling, and degradation behaviours were also investigated. The release kinetics of SC were performed. The antimicrobial analysis was evaluated against Escherichia coli and Staphylococcus aureus strains. 3D printed scaffolds have shown an excellent antibacterial effect, especially against gram-positive bacteria due to the antibacterial SC extract they contain. Furthermore, the cell viability of fibroblast (L929) cells on/within scaffolds were determined by the colourimetric MTT assay. The SA/PEG/SC scaffolds show a great promising potential candidate for diabetic wound healing and against bacterial infections. (c) 2020 Elsevier B.V. All rights reserved.Publication Metadata only Fabrication, characterization and investigation of antibacterial activity of propolis substituted sodium alginate tissue scaffolds using three-dimensional (3d) printing technology(2021-06-05) UZUN, MUHAMMET; SU TORUN, SENA; ULAĞ, SONGÜL; AKSU, MEHMET BURAK; GÜNDÜZ, OĞUZHAN; CESUR, SÜMEYYE; Aarancı K., Uzun M., Su Torun S., Cesur S., Ulağ S., Amin A., Güncü M. M., Aksu M. B., Kolaylı S., Silva J., et al.Publication Open Access Fabrication of gentamicin sulfate-loaded 3d-printed polyvinyl alcohol/sodium alginate/gelatin-methacryloyl hybrid scaffolds for skin tissue replacement(2023-01-01) ULAĞ, SONGÜL; ŞAHİN, ALİ; AKSU, MEHMET BURAK; GÜNDÜZ, OĞUZHAN; Izgordu M. S., Ayran M., ULAĞ S., Yildirim R., Bulut B., ŞAHİN A., Guncu M. M., AKSU M. B., GÜNDÜZ O.3D-printed scaffolds can better mimic the function of human skin, both biologically and mechanically. Within the scope of this study, the effect of the addition of different amounts (10, 15, 20 mg) of gentamicin sulfate (GS) to a 10 mL solution of natural and synthetic polymers is investigated. Sodium alginate (SA), gelatin-methacryloyl (GelMA), and polyvinyl alcohol (PVA) are chosen as bioactive materials. The surface morphology and pore structures are visualized by scanning electron microscopy (SEM). According to the results, it is observed that the pore sizes of all scaffolds are smaller than 270 µm, the lowest value (130 µm) is obtained in the scaffold loaded with 15 mg GS, and it also has the highest tensile strength value (12.5 ± 7.6 MPa). Similarly, it is observed that the tensile strength (9.7 ± 4.5 MPa) is high in scaffold loaded with 20 mg GS. The biocompatibility test is performed with fibroblast cells, and the results show that the scaffolds are biocompatible with cells. The antibacterial test is carried out against the S.aureous and E. coli and the results indicate that all GS-loaded scaffolds demonstrate antibacterial activity.Publication Metadata only Patch-Based Technology for Corneal Microbial Keratitis(Springer, 2020) AKSU, MEHMET BURAK; Ulag S., Ilhan E., Aksu B., Sengor M., Ekren N., Kilic O., Gunduz O.Corneal opacities, which happened mainly due to microbial keratitis, are the fourth cause of blindness worldwide. Antimicrobial therapy is an alternative solution for microbial keratitis caused by Staphylococcus aureus and Pseudomonas Aeruginosa. The aim of this study, to develop patches for the treatment of corneal keratitis which caused significant corneal blindness by using electrospinning method. Polyvinyl-alcohol (PVA) patches with Gelatine (GEL) studied in various ratios. Different amounts of gelatine added to PVA to resemble the collagen fibril structure of the cornea. To enable the patches to the antimicrobial effect against the bacterias, the special plant extract was used. The produced corneal patches were examined separately for chemical, morphological, and antimicrobial properties. Scanning electron microscope (SEM), Fourier-transform infrared (FT-IR) spectroscopy were performed to observe the surface morphology and chemical structure of the patches, respectively. © Springer Nature Switzerland AG 2020.Publication Open Access 3D Propolis-Sodium Alginate Scaffolds: Influence on Structural Parameters, Release Mechanisms, Cell Cytotoxicity and Antibacterial Activity(MDPI, 2020-11-02) AKSU, MEHMET BURAK; Aranci, Kubra; Uzun, Muhammet; Su, Sena; Cesur, Sumeyye; Ulag, Songul; Amin, Al; Guncu, Mehmet Mucahit; Aksu, Burak; Kolayli, Sevgi; Ustundag, Cem Bulent; Silva, Jorge Carvalho; Ficai, Denisa; Ficai, Anton; Gunduz, OguzhanIn this study, the main aim was to fabricate propolis (Ps)-containing wound dressing patches using 3D printing technology. Different combinations and structures of propolis (Ps)-incorporated sodium alginate (SA) scaffolds were developed. The morphological studies showed that the porosity of developed scaffolds was optimized when 20% (v/v) of Ps was added to the solution. The pore sizes decreased by increasing Ps concentration up to a certain level due to its adhesive properties. The mechanical, swelling-degradation (weight loss) behaviors, and Ps release kinetics were highlighted for the scaffold stability. An antimicrobial assay was employed to test and screen antimicrobial behavior of Ps against Escherichia coli and Staphylococcus aureus strains. The results show that the Ps-added scaffolds have an excellent antibacterial activity because of Ps compounds. An in vitro cytotoxicity test was also applied on the scaffold by using the extract method on the human dermal fibroblasts (HFFF2) cell line. The 3D-printed SA-Ps scaffolds are very useful structures for wound dressing applications.Publication Open Access Propolis-Based Nanofiber Patches to Repair Corneal Microbial Keratitis(MDPI, 2021-04-28) ŞAHİN, ALİ; Ulag, Songul; Ilhan, Elif; Demirhan, Ramazan; Sahin, Ali; Yilmaz, Betul Karademir; Aksu, Burak; Sengor, Mustafa; Ficai, Denisa; Titu, Aurel Mihail; Ficai, Anton; Gunduz, OguzhanIn this research, polyvinyl-alcohol (PVA)/gelatin (GEL)/propolis (Ps) biocompatible nanofiber patches were fabricated via electrospinning technique. The controlled release of Propolis, surface wettability behaviors, antimicrobial activities against the S. aureus and P. aeruginosa, and biocompatibility properties with the mesenchymal stem cells (MSCs) were investigated in detail. By adding 0.5, 1, and 3 wt.% GEL into the 13 wt.% PVA, the morphological and mechanical results suggested that 13 wt.% PVA/0.5 wt.% GEL patch can be an ideal matrix for 3 and 5 wt.% propolis addition. Morphological results revealed that the diameters of the electrospun nanofiber patches were increased with GEL (from 290 nm to 400 nm) and Ps addition and crosslinking process cause the formation of thicker nanofibers. The tensile strength and elongation at break enhancement were also determined for 13 wt.% PVA/0.5 wt.% GEL/3 wt.% Ps patch. Propolis was released quickly in the first hour and arrived at a plateau. Cell culture and contact angle results confirmed that the 3 wt.% addition of propolis reinforced mesenchymal stem cell proliferation and wettability properties of the patches. The antimicrobial activity demonstrated that propolis loaded patches had antibacterial activity against the S. aureus, but for P. aeruginosa, more studies should be performed.Publication Open Access Co-Culture of Keratinocyte-Staphylococcus aureus on Cu-Ag-Zn/CuO and Cu-Ag-W Nanoparticle Loaded Bacterial Cellulose:PMMA Bandages(WILEY-V C H VERLAG GMBH, 2019-01) YILMAZ, BETÜL; Altun, Esra; Aydogdu, Mehmet Onur; Crabbe-Mann, Maryam; Ahmed, Jubair; Brako, Francis; Karademir, Betul; Aksu, Burak; Sennaroglu, Muge; Eroglu, Mehmet S.; Ren, Guogang; Gunduz, Oguzhan; Edirisinghe, MohanPressurized gyration and its sister processes are novel methods to produce polymeric fibers. Potential applications for such fibers include wound dressings, tissue engineering scaffolds, and filters. This study reports on a pressurized gyration technique that employs pressured N-2 gas to prepare biocompatible wound dressing bandages from bacterial cellulose and poly (methylmethacrylate) polymer blended with alloyed antimicrobial nanoparticles. Resulting bandages are manufactured with high product yield and characterized for their chemical, physical, and mechanical properties. Increased density in solutions with additional antimicrobial nanoparticles results in increased fiber diameters. Also, addition of antimicrobial nanoparticles enhances ultimate tensile strength and Young's modulus of the bandages. Typical molecular bonding in the bandages is confirmed by Fourier-transform infrared spectroscopy, with peaks that have higher intensity and narrowing points being caused by additional antimicrobial nanoparticles. More so, the cellular response to the bandages and the accompanying antimicrobial activity are studied in detail by in vitro co-culture of Staphylococcus aureus and keratinocytes. Antimicrobial nanoparticle-loaded bandage samples show increased cell viability and bacteria inhibition during co-culture and are found to have a promising future as epidermal wound dressing materials.Publication Metadata only A novel approach to treat the Thiel-Behnke corneal dystrophy using 3D printed honeycomb-shaped polymethylmethacrylate (PMMA)/Vancomycin (VAN) scaffolds(Elsevier B.V., 2021) ŞAHİN, ALİ; Ulag S., Sahin A., Guncu M.M., Aksu B., Ekren N., Sengor M., Kalaskar D.M., Gunduz O.Thiel-Behnke corneal dystrophy, or honeycomb corneal dystrophy, is an autosomal dominant corneal disorder. Tissue engineering can be a novel approach to regenerate this dystrophy. In this study, the honeycomb geometry of the dystrophy mimicked with a 3D printing technology, and 40% PMMA, 40% PMMA/(0.1, 0.5, 2, and 10)% VAN scaffolds were fabricated with honeycomb geometry. As a result of the biocompatibility test with mesenchymal stem cells (MSCs), it can be said that cells on the scaffolds showed high viability and proliferation for all incubation periods. According to the antibacterial activity results, the 40% PMMA/10% VAN showed antibacterial activity against S. aureous. Mechanical results reported that with the addition of VAN into the 40% PMMA, the tensile strength value increased up to 2% VAN amount. The swelling behaviours of the scaffolds were examined in vitro, and found that the swelling rate increased with a high VAN amount. The release of VAN from the scaffolds showed sustained release behaviour, and it took 13 days to be released entirely from the scaffolds. © 2021 Elsevier B.V.