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CD36 expression in peripheral blood mononuclear cells reflects the onset of atherosclerosis

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dc.contributor.authorSÖZEN, AHMET ERDİ
dc.contributor.authorsYazgan, Burak; Sozen, Erdi; Karademir, Betul; Ustunsoy, Seyfettin; Ince, Umit; Zarkovic, Neven; Ozer, Nesrin Kartal
dc.date.accessioned2022-03-12T22:26:57Z
dc.date.available2022-03-12T22:26:57Z
dc.date.issued2018
dc.description.abstractTogether with complex genetic and environmental factors, increased serum cholesterol and ox-LDL levels are considered as major triggering factors of atherosclerosis. Mononuclear cell infiltration to the arterial wall and uptake of ox-LDL, which is facilitated by CD36 receptor through an uncontrolled manner, play a key role in foam cell formation followed by atherogenesis development. The aim of this study was to analyze if CD36 expression in peripheral blood mononuclear cells reflect its aortic tissue level in hypercholesterolemia. In this study, CD36 protein expression was evaluated in aortic specimens of cholesterol or cholesterol plus Vitamin E treated animals in relation to the immunohistochemical analyses for the HNE-protein adducts, as well as for smooth muscle actin and vimentin. The CD36 mRNA expression was determined by RT-PCR in PBMC of hypercholesterolemic rabbits and hypercholesterolemic versus normocholesterolemic individuals. Immunohistochemistry findings revealed that smooth muscle actin, smooth muscle vimentin, HNE-protein conjugates, and CD36 protein expressions were significantly increased in aorta of hypercholesterolemic group where foam cells were present. High cholesterol diet significantly induced CD36 mRNA expression in both rabbit aorta and PBMCs, while positive correlation between aortic and PBMC CD36 expression has been found. In addition, consistent with the rabbit model, CD36 mRNA expression levels in human PBMCs were significantly higher in hypercholesterolemic patients than in normocholesterolemic individuals. Taken together, these results demonstrate that the CD36 mRNA levels of PBMCs could reflect the CD36 mRNA levels in aorta and could be used as a biomarker for diagnosis of atherosclerotic burden. (c) 2018 BioFactors, 44(6):588-596, 2018
dc.identifier.doi10.1002/biof.1372
dc.identifier.eissn1872-8081
dc.identifier.issn0951-6433
dc.identifier.pubmed28677864
dc.identifier.urihttps://hdl.handle.net/11424/235139
dc.identifier.wosWOS:000453518800009
dc.language.isoeng
dc.publisherWILEY
dc.relation.ispartofBIOFACTORS
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjecthypercholesterolemia
dc.subjectatherosclerosis
dc.subjectPBMC
dc.subjectCD36 receptor
dc.subjectSCAVENGER RECEPTOR EXPRESSION
dc.subjectVITAMIN-E
dc.subjectOXIDATIVE STRESS
dc.subjectSOLUBLE CD36
dc.subjectOXIDIZED LDL
dc.subjectCARDIOVASCULAR-DISEASE
dc.subjectINSULIN-RESISTANCE
dc.subjectREDOX REGULATION
dc.subjectLIPID RAFTS
dc.subjectCHOLESTEROL
dc.titleCD36 expression in peripheral blood mononuclear cells reflects the onset of atherosclerosis
dc.typearticle
dspace.entity.typePublication
local.avesis.id5fad835c-09b8-46e2-9b17-d9369b0f3463
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages9
local.journal.quartileQ2
oaire.citation.endPage596
oaire.citation.issue6
oaire.citation.startPage588
oaire.citation.titleBIOFACTORS
oaire.citation.volume44
relation.isAuthorOfPublication47991a21-cce9-4b87-b091-bede64f8b4e0
relation.isAuthorOfPublication.latestForDiscovery47991a21-cce9-4b87-b091-bede64f8b4e0

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