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Levansucrase from Halomonas smyrnensis AAD6(T): first halophilic GH-J clan enzyme recombinantly expressed, purified, and characterized

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dc.contributor.authorTOKSOY ÖNER, EBRU
dc.contributor.authorsKirtel, Onur; Menendez, Carmen; Versluys, Maxime; Van den Ende, Wim; Hernandez, Lazaro; Oner, Ebru Toksoy
dc.date.accessioned2022-03-14T10:17:02Z
dc.date.available2022-03-14T10:17:02Z
dc.date.issued2018-11
dc.description.abstractFructans, homopolymers of fructose produced by fructosyltransferases (FTs), are emerging as intriguing components in halophiles since they are thought to be associated with osmotic stress tolerance and overall fitness of microorganisms and plants under high-salinity conditions. Here, we report on the full characterization of the first halophilic FT, a levansucrase from Halomonas smyrnensis AAD6(T) (HsLsc; EC 2.4.1.10). The encoding gene (lsc) was cloned into a vector with a 6xHis Tag at its C-terminus, then expressed in Escherichia coli. The purified recombinant enzyme (47.3kDa) produces levan and a wide variety of fructooligosaccharides from sucrose, but only in the presence of high salt concentrations (>1.5M NaCl). HsLsc showed Hill kinetics and pH and temperature optima of 5.9 and 37 degrees C, respectively. Interestingly, HsLsc was still very active at salt concentrations close to saturation (4.5M NaCl) and was selectively inhibited by divalent cations. The enzyme showed high potential in producing novel saccharides derived from raffinose as both fructosyl donor and acceptor and cellobiose, lactose, galactose, and -arabinose as fructosyl acceptors. With its unique biochemical characteristics, HsLsc is an important enzyme for future research and potential industrial applications in a world faced with drought and diminishing freshwater supplies.
dc.identifier.doi10.1007/s00253-018-9311-z
dc.identifier.eissn1432-0614
dc.identifier.issn0175-7598
dc.identifier.pubmed30120521
dc.identifier.urihttps://hdl.handle.net/11424/244301
dc.identifier.wosWOS:000448359000016
dc.language.isoeng
dc.publisherSPRINGER
dc.relation.ispartofAPPLIED MICROBIOLOGY AND BIOTECHNOLOGY
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectLevansucrase
dc.subjectFructosyltransferase
dc.subjectHalophilic enzyme
dc.subjectHalomonas smyrnensis
dc.subjectLevan
dc.subjectFructan
dc.subjectBACILLUS-SUBTILIS LEVANSUCRASE
dc.subjectZYMOMONAS-MOBILIS
dc.subjectFRUCTOSYLTRANSFERASE LEVANSUCRASE
dc.subjectFRUCTO-OLIGOSACCHARIDES
dc.subjectINULOSUCRASE ENZYMES
dc.subjectERWINIA-AMYLOVORA
dc.subjectCRYSTAL-STRUCTURE
dc.subjectLEVAN
dc.subjectSUCROSE
dc.subjectBIOSYNTHESIS
dc.titleLevansucrase from Halomonas smyrnensis AAD6(T): first halophilic GH-J clan enzyme recombinantly expressed, purified, and characterized
dc.typearticle
dspace.entity.typePublication
local.avesis.id4ecd9c29-3316-4010-90ac-b0dfacae0874
local.import.packageSS16
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages14
local.journal.quartileQ2
oaire.citation.endPage9220
oaire.citation.issue21
oaire.citation.startPage9207
oaire.citation.titleAPPLIED MICROBIOLOGY AND BIOTECHNOLOGY
oaire.citation.volume102
relation.isAuthorOfPublication6118e9e4-a58e-429b-bbec-4f73a3089a2b
relation.isAuthorOfPublication.latestForDiscovery6118e9e4-a58e-429b-bbec-4f73a3089a2b

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