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Molasses as fermentation substrate for levan production by Halomonas sp.

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dc.contributor.authorTOKSOY ÖNER, EBRU
dc.contributor.authorsKucukasik, Faruk; Kazak, Hande; Guney, Dilvin; Finore, Ilaria; Poli, Annarita; Yenigun, Orhan; Nicolaus, Barbara; Oner, Ebru Toksoy
dc.date.accessioned2022-03-12T18:04:54Z
dc.date.available2022-03-12T18:04:54Z
dc.date.issued2011
dc.description.abstractLevan is a homopolymer of fructose with many outstanding properties like high solubility in oil and water, strong adhesiveness, good biocompatibility, and film-forming ability. However, its industrial use has long been hampered by costly production processes which rely on mesophilic bacteria and plants. Recently, Halomonas sp. AAD6 halophilic bacteria were found to be the only extremophilic species producing levan at high titers in semi-chemical medium containing sucrose, and in this study, pretreated sugar beet molasses and starch molasses were both found to be feasible substitutes for sucrose. Five different pretreatment methods and their combinations were applied to both molasses types. Biomass and levan concentrations reached by the Halomonas sp. AAD6 cells cultivated on 30 g/L of pretreated beet molasses were 6.09 g dry cells/L and 12.4 g/L, respectively. When compared with literature, Halomonas sp. was found to stand out with its exceptionally high levan production yields on available fructose. Molecular characterization and monosaccharide composition studies confirmed levan-type fructan structure of the biopolymers. Rheological properties under different conditions pointed to the typical characteristics of low viscosity and pseudoplastic behaviors of the levan polymers. Moreover, levan polymer produced from molasses showed high biocompatibility and affinity with both cancerous and non-cancerous cell lines.
dc.identifier.doi10.1007/s00253-010-3055-8
dc.identifier.eissn1432-0614
dc.identifier.issn0175-7598
dc.identifier.pubmed21161209
dc.identifier.urihttps://hdl.handle.net/11424/230510
dc.identifier.wosWOS:000288333800008
dc.language.isoeng
dc.publisherSPRINGER
dc.relation.ispartofAPPLIED MICROBIOLOGY AND BIOTECHNOLOGY
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectLevan
dc.subjectFructan
dc.subjectHalomonas
dc.subjectMolasses
dc.subjectSugar beet
dc.subjectExopolysaccharide
dc.subjectSTRUCTURAL-CHARACTERIZATION
dc.subjectACID PRODUCTION
dc.subjectGUM PRODUCTION
dc.subjectCANE MOLASSES
dc.subjectTRACE-METALS
dc.subjectEXOPOLYSACCHARIDES
dc.subjectOPTIMIZATION
dc.subjectPOLYSACCHARIDES
dc.subjectPRETREATMENT
dc.subjectNUTRIENTS
dc.titleMolasses as fermentation substrate for levan production by Halomonas sp.
dc.typearticle
dspace.entity.typePublication
local.avesis.id072ed42c-d7df-4bd8-97ce-572aef5b558c
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.journal.numberofpages12
oaire.citation.endPage1740
oaire.citation.issue6
oaire.citation.startPage1729
oaire.citation.titleAPPLIED MICROBIOLOGY AND BIOTECHNOLOGY
oaire.citation.volume89
relation.isAuthorOfPublication6118e9e4-a58e-429b-bbec-4f73a3089a2b
relation.isAuthorOfPublication.latestForDiscovery6118e9e4-a58e-429b-bbec-4f73a3089a2b

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