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Cytotoxic Antibody Detection by Means of Flow-Cytometric Cross-Match

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dc.contributor.authorATA, PINAR
dc.contributor.authorsBilgen, T.; Ata, P.; Tozkir, J.; Tozkir, H.; Titiz, M. I.
dc.date.accessioned2022-03-12T22:23:42Z
dc.date.available2022-03-12T22:23:42Z
dc.date.issued2017
dc.description.abstractBackground. Complement-dependent lymphocytotoxicity (CDC-XM) and flowcytometric (FCXM) cross-match are analyzed individually for each donor and recipient pair, because these techniques have fundamental differences for the evaluation of histocompatibility. Lately, cytotoxic flow-cytometric cross-match (cFCXM) has been developed as an alternative to both CDC-XM and FCXM techniques. We evaluated the limits of cFCXM with the use of different positive serum dilutions. Methods. CDC-XM, FCXM, and cFCXM tests were performed with the use of commercially available negative and positive serum samples and lymphocytes from healthy donors. Results. Complement-dependent cell death was successfully detected with the use of cFCXM. Complement-dependent cell death ratios in cFCXM were similar those in CDCXM. With cFCXM, not only complement-dependent cell death but also IgG binding could be detected within a single assay. At higher concentrations of the positive serum, IgG-fluorescein isothiocyanate (FITC) mean fluorescent intensity (MFI) values detected with the use of cFCXM were less than those of conventional FCXM. Correspondingly, for dead cells, MFI values of IgG-FITC were less than those of live cells in higher positive serum concentrations in the cFCXM assay. Moreover, our results demonstrated that in cFCXM analysis, the decreasing ratio of dead cells at increasing positive serum dilutions was not in parallel with the same decrease in IgG-FITC MFI values. Conclusions. The cFCXM technique detects complement-mediated cytotoxic cell death with the additional ability to show IgG binding in the same tube and therefore may reduce the necessary bench time and workload.
dc.identifier.doi10.1016/j.transproceed.2017.01.016
dc.identifier.eissn1873-2623
dc.identifier.issn0041-1345
dc.identifier.pubmed28340808
dc.identifier.urihttps://hdl.handle.net/11424/234522
dc.identifier.wosWOS:000398017900012
dc.language.isoeng
dc.publisherELSEVIER SCIENCE INC
dc.relation.ispartofTRANSPLANTATION PROCEEDINGS
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectRENAL-TRANSPLANTATION
dc.subjectBINDING
dc.subjectASSAY
dc.subjectIGG
dc.titleCytotoxic Antibody Detection by Means of Flow-Cytometric Cross-Match
dc.typearticle
dspace.entity.typePublication
local.avesis.id4b2c9291-0e75-4539-bab7-a631498beebb
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages5
local.journal.quartileQ4
oaire.citation.endPage444
oaire.citation.issue3
oaire.citation.startPage440
oaire.citation.titleTRANSPLANTATION PROCEEDINGS
oaire.citation.volume49
relation.isAuthorOfPublication4ef620f9-0462-4687-84fa-c983fb788878
relation.isAuthorOfPublication.latestForDiscovery4ef620f9-0462-4687-84fa-c983fb788878

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