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Fructans Prime ROS Dynamics and Botrytis cinerea Resistance in Arabidopsis

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dc.contributor.authorTOKSOY ÖNER, EBRU
dc.contributor.authorsJanse van Rensburg, Henry Christopher; Takacs, Zoltan; Freynschlag, Florentina; Toksoy Oner, Ebru; Jonak, Claudia; Van den Ende, Wim
dc.date.accessioned2022-03-14T10:53:44Z
dc.date.available2022-03-14T10:53:44Z
dc.date.issued2020-09-01
dc.description.abstractNaturally derived molecules can be used as priming or defense stimulatory agents to protect against biotic stress. Fructans have gained strong interest due to their ability to induce resistance in a number of crop species. In this study, we set out to establish the role of fructan-induced immunity against the fungal pathogen Botrytis cinerea in Arabidopsis thaliana. We show that both inulin- and levan-type fructans from different sources can enhance Arabidopsis resistance against B. cinerea. We found that inulin from chicory roots and levan oligosaccharides from the exopolysaccharide-producing bacterium Halomonas smyrnensis primed the NADPH-oxidase-mediated reactive oxygen species (ROS) burst in response to the elicitors flg22, derived from the bacterial flagellum, and oligogalacturonides (OGs), derived from the host cell wall. Neither induced a direct ROS burst typical of elicitors. We also found a primed response after infection with B. cinerea for H2O2 accumulation and the activities of ascorbate peroxidase and catalase. Sucrose accumulated as a consequence of fructan priming, and glucose and sucrose levels increased in fructan-treated plants after infection with B. cinerea. This study shows that levan-type fructans, specifically from bacterial origin, can prime plant defenses and that both inulin and levan oligosaccharide-mediated priming is associated with changes in ROS dynamics and sugar metabolism. Establishing fructan-induced immunity in Arabidopsis is an important step to further study the underlying mechanisms since a broad range of biological resources are available for Arabidopsis.
dc.identifier.doi10.3390/antiox9090805
dc.identifier.eissn2076-3921
dc.identifier.pubmed32882794
dc.identifier.urihttps://hdl.handle.net/11424/245355
dc.identifier.wosWOS:000581662600001
dc.language.isoeng
dc.publisherMDPI
dc.relation.ispartofANTIOXIDANTS
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectfructan
dc.subjectBotrytis cinerea
dc.subjectArabidopsis thaliana
dc.subjectpriming
dc.subjectreactive oxygen species
dc.subjectsweet immunity
dc.subjectsugars
dc.subjectBETA-AMINOBUTYRIC ACID
dc.subjectPLANT DEFENSE RESPONSES
dc.subjectNADPH OXIDASE RBOHD
dc.subjectOXIDATIVE BURST
dc.subjectINFECTION
dc.subjectSTRESS
dc.subjectLEAVES
dc.subjectTOMATO
dc.subjectSUGARS
dc.subjectIMMUNITY
dc.titleFructans Prime ROS Dynamics and Botrytis cinerea Resistance in Arabidopsis
dc.typearticle
dspace.entity.typePublication
local.avesis.idb047e35c-187e-4a5a-95e7-090f0ad93850
local.import.packageSS16
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.articlenumber805
local.journal.numberofpages22
local.journal.quartileQ1
oaire.citation.issue9
oaire.citation.titleANTIOXIDANTS
oaire.citation.volume9
relation.isAuthorOfPublication6118e9e4-a58e-429b-bbec-4f73a3089a2b
relation.isAuthorOfPublication.latestForDiscovery6118e9e4-a58e-429b-bbec-4f73a3089a2b

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