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High bioreactor production and emulsifying activity of an unusual exopolymer by Chromohalobacter canadensis 28

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dc.contributor.authorTOKSOY ÖNER, EBRU
dc.contributor.authorsRadchenkova, Nadja; Boyadzhieva, Ivanka; Haskoylu, Merve Erginer; Atanasova, Nikolina; Yildiz, Songul Yasar; Kuncheva, Margarita J.; Panchev, Ivan; Kisov, Hristo; Vassilev, Spasen; Oner, Ebru Toksoy; Kambourova, Margarita S.
dc.date.accessioned2022-03-14T09:22:10Z
dc.date.available2022-03-14T09:22:10Z
dc.date.issued2020-08
dc.description.abstractUnusual composition of an exopolymer (EP) from an obligate halophilic bacterium Chromohalobacter canadensis 28 has triggered an interest in development of an effective bioreactor process for its production. Its synthesis was investigated in 2-L bioreactor at agitation speeds at interval 600-1000 rpm, at a constant air flow rate of 0.5 vvm; aeration rates of 0.5, 1.0, and 1.5 vvm were tested at constant agitation rate of 900 rpm. EP production was affected by both, agitation and aeration. As a result twofold increase of EP yield was observed and additionally increased up to 3.08 mg/mL in a presence of surfactants. For effective scale-up of bioreactors mass transfer parameters were estimated and lowest values of K(L)a obtained for the highest productivity fermentation was established. Emulsification activity of EP exceeded that of trade hydrocolloids xanthan, guar gum, and cellulose. A good synergism between EP and commercial cellulose proved its potential exploration as an enhancer of emulsifying properties of trade emulsions. A pronounced lipophilic effect of EP was established toward olive oil and liquid paraffin. Cultivation of human keratinocyte cells (HaCaT) with crude EP and purified gamma-polyglutamic acid (PGA) showed higher viability than control group.
dc.identifier.doi10.1002/elsc.202000012
dc.identifier.eissn1618-2863
dc.identifier.issn1618-0240
dc.identifier.pubmed32774208
dc.identifier.urihttps://hdl.handle.net/11424/243033
dc.identifier.wosWOS:000536833400001
dc.language.isoeng
dc.publisherWILEY
dc.relation.ispartofENGINEERING IN LIFE SCIENCES
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectagitation and aeration
dc.subjectbiocompatibility
dc.subjectemulsifying properties
dc.subjectexopolymer
dc.subjecthalophilic bacterium
dc.subjectEXOPOLYSACCHARIDE PRODUCTION
dc.subjectAGITATION SPEED
dc.subjectAERATION
dc.subjectOPTIMIZATION
dc.subjectEPS
dc.titleHigh bioreactor production and emulsifying activity of an unusual exopolymer by Chromohalobacter canadensis 28
dc.typearticle
dspace.entity.typePublication
local.avesis.id213992c1-8b7d-4cb7-af2a-a34137f6b16e
local.import.packageSS16
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages11
local.journal.quartileQ3
oaire.citation.endPage367
oaire.citation.issue8
oaire.citation.startPage357
oaire.citation.titleENGINEERING IN LIFE SCIENCES
oaire.citation.volume20
relation.isAuthorOfPublication6118e9e4-a58e-429b-bbec-4f73a3089a2b
relation.isAuthorOfPublication.latestForDiscovery6118e9e4-a58e-429b-bbec-4f73a3089a2b

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