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Interaction of influenza A virus NS2/NEP protein with the amino-terminal part of Nup214

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dc.contributor.authorTURAN, KADİR
dc.contributor.authorsSenbas Akyazi, Burcak; Pirincal, Aysegul; Kawaguchi, Atsushi; Nagata, Kyosuke; Turan, Kadir
dc.date.accessioned2022-04-25T00:11:42Z
dc.date.available2022-04-25T00:11:42Z
dc.date.issued2020
dc.description.abstractInfluenza A viruses have a single-stranded RNA genome consisting of 8 segments. Each RNA segment associates with the nucleoprotein (NP) and viral RNA polymerase to and from a viral ribonucleoprotein (vRNP) particle. The viral mRNA synthesis is dependent on a capped pruner derived from nascent host RNA transcripts. For these processes to take place, vRNPs must pass through the cell nuclear pore complex (NPC) to the nucleus. The influenza A virus NS2 protein, also called the nuclear export protein (NES), has an important role in the nucleocytoplasmic transport of vRNPs. This protein interacts with the host cellular nucleoporins during the nuclear export of vRNPs. In this study, the human nucleoporin 214 (Nup214) was identified as an NS2-binding protein by using a yeast two-hybrid assay. The interaction between NS2 and human Nup214 was confirmed in both yeast and mammalian cells. It has been shown that the NS2 protein interacts with the amino terminal FG domain of the Nup214 protein. The influenza viral replication was suppressed in knockdown cells for the Nup214 protein. It was concluded that the FG domains of nucleoporins have an important role in the interaction of the influenza NS2 protein with host NPC for vRNA export.
dc.identifier.doi10.3906/biy-1909-49
dc.identifier.eissn1303-6092
dc.identifier.issn1300-0152
dc.identifier.pubmed32256144
dc.identifier.urihttps://hdl.handle.net/11424/263953
dc.identifier.wosWOS:000524275900003
dc.languageeng
dc.publisherTUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEY
dc.relation.ispartofTURKISH JOURNAL OF BIOLOGY
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectInfluenza A viruses
dc.subjectNS2/NEP
dc.subjectNup214
dc.subjectnuclear pore complex
dc.subjectnuclear export
dc.subjectNUCLEAR-PORE COMPLEX
dc.subjectNUCLEOCYTOPLASMIC TRANSPORT
dc.subjectFUNCTIONAL-ANALYSIS
dc.subjectNUCLEOPORIN NUP214
dc.subjectEXPORT PROTEIN
dc.subjectM1 PROTEIN
dc.subjectDOMAIN
dc.subjectIMPORT
dc.subjectOVEREXPRESSION
dc.subjectPOLYMERASE
dc.titleInteraction of influenza A virus NS2/NEP protein with the amino-terminal part of Nup214
dc.typearticle
dspace.entity.typePublication
local.avesis.id2f9e02e1-9476-4b38-9b46-dc55047e2f21
local.import.packageSS39
local.indexed.atWOS
local.journal.numberofpages11
local.journal.quartileQ3
oaire.citation.endPage92
oaire.citation.issue2
oaire.citation.startPage82
oaire.citation.titleTURKISH JOURNAL OF BIOLOGY
oaire.citation.volume44
relation.isAuthorOfPublication9cc23fc2-5031-46cd-91a0-a2e13ab788d4
relation.isAuthorOfPublication.latestForDiscovery9cc23fc2-5031-46cd-91a0-a2e13ab788d4

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