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Vascular endothelial growth factor-loaded poly(lactic-co-glycolic acid) microspheres-induced lateral axonal sprouting into the vein graft bridging two healthy nerves: Nerve graft prefabrication using controlled release system

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dc.contributor.authorAKAKIN, DİLEK
dc.contributor.authorsKaragoz, Huseyin; Ulkur, Ersin; Kerimoglu, Oya; Alarcin, Emine; Sahin, Cihan; Akakin, Dilek; Dortunc, Betul
dc.date.accessioned2022-03-12T18:07:25Z
dc.date.available2022-03-12T18:07:25Z
dc.date.issued2012
dc.description.abstractThe most commonly used surgical technique for repairing segmental nerve defects is autogenous nerve grafting; however, this method causes donor site morbidity. In this study, we sought to produce prefabricated nerve grafts that can serve as a conduit instead of autologous nerve using a controlled release system created with vascular endothelial growth factor (VEGF)-loaded poly(lactic-co-glycolic acid) (PLGA) microspheres. The study was performed in vitro and in vivo. For the in vitro studies, VEGF-loaded PLGA microspheres were prepared. Thirty rats were used for the in vivo studies. Vein grafts were sutured between the tibial and peroneal nerves in all animals. Three groups were created, and an epineural window, partial incision, and microsphere application were performed, respectively. Walking track analysis, morphologic, and electron microscopic assessment were performed at the end of the eight weeks. Microspheres were produced in spherical shapes as required. Controlled release of VEGF was achieved during a 30-days period. Although signs of nerve injury occurred initially in the partial incision groups according to the indexes of peroneal and tibial function, it improved gradually. The index values were not affected in the other groups. There were many myelinated fibers with large diameters in the partial incision and controlled release groups, while a few myelinated fibers that passed through vein graft in the epineural window group. Thereby, prefabrication was carried out for the second and third groups. It was demonstrated that nerve graft can be prefabricated by the controlled delivery of VEGF. (c) 2012 Wiley Periodicals, Inc. Microsurgery, 2012.
dc.identifier.doi10.1002/micr.22016
dc.identifier.issn0738-1085
dc.identifier.pubmed22821743
dc.identifier.urihttps://hdl.handle.net/11424/231015
dc.identifier.wosWOS:000310965400009
dc.language.isoeng
dc.publisherWILEY-BLACKWELL
dc.relation.ispartofMICROSURGERY
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectTO-SIDE NEURORRHAPHY
dc.subjectEPINEURIAL SHEATH
dc.subjectGENE-THERAPY
dc.subjectREGENERATION
dc.subjectREPAIR
dc.subjectCONDUITS
dc.subjectDEFECTS
dc.subjectRECONSTRUCTION
dc.subjectENHANCEMENT
dc.subjectRATS
dc.titleVascular endothelial growth factor-loaded poly(lactic-co-glycolic acid) microspheres-induced lateral axonal sprouting into the vein graft bridging two healthy nerves: Nerve graft prefabrication using controlled release system
dc.typearticle
dspace.entity.typePublication
local.avesis.id286b4c89-2118-4e7a-ac15-2b8749e0d57a
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.journal.numberofpages7
oaire.citation.endPage641
oaire.citation.issue8
oaire.citation.startPage635
oaire.citation.titleMICROSURGERY
oaire.citation.volume32
relation.isAuthorOfPublicationc97c0ebf-ffce-4b27-a933-941a53767b0d
relation.isAuthorOfPublication.latestForDiscoveryc97c0ebf-ffce-4b27-a933-941a53767b0d

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