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Indocyanine green based fluorescent polymeric nanoprobes for in vitro imaging

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dc.contributor.authorYILMAZ, BETÜL
dc.contributor.authorsEge, Zeynep R.; Akan, Aydin; Oktar, Faik N.; Lin, Chi C.; Kuruca, Durdane S.; Karademir, Betul; Sahin, Yesim M.; Erdemir, Gokce; Gunduz, Oguzhan
dc.date.accessioned2022-03-12T22:54:51Z
dc.date.available2022-03-12T22:54:51Z
dc.date.issued2020
dc.description.abstractIndocyanine green (ICG) provides an advantage in the imaging of deep tumors as it can reach deeper location without being absorbed in the upper layers of biological tissues in the wavelengths, which named therapeutic window in the tissue engineering. Unfortunately, rapid elimination and short-term stability in aqueous media limited its use as a fluorescence probe for the early detection of cancerous tissue. In this study, stabilization of ICG was performed by encapsulating ICG molecules into the biodegradable polymer composited with poly(l-lactic acid) and poly(epsilon-caprolactone) via a simple one-step multiaxial electrospinning method. Different types of coaxial and triaxial structure groups were performed and compared with single polymer only groups. Confocal microscopy was used to image the encapsulated ICG (1 mg/mL) within electrospun nanofibers and in vitro ICG uptake by MIA PaCa-2 pancreatic cancer cells. Stability of encapsulated ICG is demonstrated by the in vitro sustainable release profile in PBS (pH = 4 and 7) up to 21 days. These results suggest the potential of the ability of internalization and accommodation of ICG into the pancreatic cell cytoplasm from in vitro implanted ICG-encapsulated multiaxial nanofiber mats. ICG-encapsulated multilayer nanofibers may be promising for the local sustained delivery system to eliminate loss of dosage caused by direct injection of ICG-loaded nanoparticles in systemic administration.
dc.identifier.doi10.1002/jbm.b.34410
dc.identifier.eissn1552-4981
dc.identifier.issn1552-4973
dc.identifier.pubmed31087780
dc.identifier.urihttps://hdl.handle.net/11424/236555
dc.identifier.wosWOS:000505634000022
dc.language.isoeng
dc.publisherWILEY
dc.relation.ispartofJOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectcontrolled release
dc.subjectencapsulation
dc.subjectindocyanine green
dc.subjectmultiaxial electrospinning
dc.subjectnear-infrared nanoprobe
dc.subjectCONTROLLED-RELEASE
dc.subjectDRUG-DELIVERY
dc.subjectNANOPARTICLES
dc.subjectNANOFIBERS
dc.subjectENCAPSULATION
dc.subjectPEG
dc.subjectCELLULOSE
dc.subjectOXIDE
dc.subjectICG
dc.titleIndocyanine green based fluorescent polymeric nanoprobes for in vitro imaging
dc.typearticle
dspace.entity.typePublication
local.avesis.id9a674f98-df37-4c16-8daf-7dffc47e3ccf
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages17
local.journal.quartileQ2
oaire.citation.endPage554
oaire.citation.issue2
oaire.citation.startPage538
oaire.citation.titleJOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
oaire.citation.volume108
relation.isAuthorOfPublication81633a07-e5fb-4760-b3ef-bf0878d87827
relation.isAuthorOfPublication.latestForDiscovery81633a07-e5fb-4760-b3ef-bf0878d87827

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