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A SIMPLIFIED METHOD FOR THE EXTRACTION OF RECOMBINANT TAQ DNA POLYMERASE FROM ESCHERICHIA COLI

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dc.contributor.authorTURAN, KADİR
dc.contributor.authorsTuran, Kadir; Eken, Burak
dc.date.accessioned2022-03-14T09:03:13Z
dc.date.available2022-03-14T09:03:13Z
dc.date.issued2018-04-01
dc.description.abstractDNA polymerase (Taq) enzyme isolated from Thermus aquaticus, a thermostable gram-negative bacterium, is a basic component of PCR, widely used in life sciences. The extraction and purification of this enzyme involves time-consuming and expensive steps such as precipitation of proteins with PEI and/or ammonium sulfate, column chromatography techniques, and removal of salts or other small molecular weight contaminants by dialysis. In this work, a novel and simplified method for extraction and purification of the recombinant Taq polymerase from Escherichia coli was employed, which used cold acetone instead of PEI or ammonium sulfate to precipitate the enzyme. The enzyme was efficiently recovered as active form from both the crude cell lysate and column fractions with cold acetone precipitation. This simplified method enabled us to obtain high quality Taq DNA polymerase in a much shorter time and at a lower cost.
dc.identifier.doi10.15414/jmbfs.2018.7.5.445-448
dc.identifier.issn1338-5178
dc.identifier.urihttps://hdl.handle.net/11424/242234
dc.identifier.wosWOS:000455216600001
dc.language.isoeng
dc.publisherSLOVAK UNIV AGRICULTURE NITRA
dc.relation.ispartofJOURNAL OF MICROBIOLOGY BIOTECHNOLOGY AND FOOD SCIENCES
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectTaq DNA polymerase
dc.subjectAcetone precipitation
dc.subjectPCR
dc.subjectThermus aquaticus
dc.subjectENZYMATIC AMPLIFICATION
dc.subjectRAPID PURIFICATION
dc.subjectEXPRESSION
dc.subjectBINDING
dc.subjectGENE
dc.subjectTEMPERATURE
dc.titleA SIMPLIFIED METHOD FOR THE EXTRACTION OF RECOMBINANT TAQ DNA POLYMERASE FROM ESCHERICHIA COLI
dc.typearticle
dspace.entity.typePublication
local.avesis.ida7d0ea25-3858-41bb-95f6-781450f56e15
local.import.packageSS16
local.indexed.atWOS
local.indexed.atSCOPUS
local.journal.numberofpages4
oaire.citation.endPage448
oaire.citation.issue5
oaire.citation.startPage445
oaire.citation.titleJOURNAL OF MICROBIOLOGY BIOTECHNOLOGY AND FOOD SCIENCES
oaire.citation.volume7
relation.isAuthorOfPublication9cc23fc2-5031-46cd-91a0-a2e13ab788d4
relation.isAuthorOfPublication.latestForDiscovery9cc23fc2-5031-46cd-91a0-a2e13ab788d4

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