Publication: A novel strategy as a potential rapid therapy modality in the treatment of corneal ulcers: Fluconazole/vancomycin dual drug-loaded nanofibrous patches
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Date
2023-01-01
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Abstract
Corneal ulcer, which is brought on by a breach in the epithelial barrier, is a dangerous infection of the avascular corneal stroma. New treatment strategies are needed, suppressing the aggressive nature of the disease and including a combination of different drugs. In this study, vancomycin (VAN) and fluconazole (FLU) dual-drug loaded dual-layered polyvinyl alcohol and gelatin (PVA/GEL) nanofibrous patches are produced by electrospinning. Scanning electron microscopy (SEM) images show smooth surfaces are obtained for both pure and drug-loaded nanofibrous patches. The tensile test results report that loading the FLU and VAN separately into the PVA/GEL patches decrease both the tensile strength and elongation at break and it is further reduced when combining two drug-loaded layers in one patch. According to drug release results, the FLU and VAN-loaded nanofibrous patches show a controlled release profile extending up to 96 h. Moreover, PVA/GEL/FLU, PVA/GEL/VAN, and PVA/GEL/FLU/VAN nanofibrous patches display significant antimicrobial activity against Candida albicans and Staphylococcus aureus. SEM, 4\"-6diamidynofenyloindol (DAPI) staining, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay show that PVA/FLU and PVA/GEL/FLU/VAN nanofibrous patches have a superior effect on NIH3T3 cell spreading and proliferation. The novelty of this study lays in the development of a potential dual drug rapid treatment for corneal ulcers of aggressive nature.
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Kimya Mühendisliği ve Teknolojisi, Kimya, Biyokimya, Biyoinorganik Kimya, Fizikokimya, Polimer Karakterizasyonu, Temel Bilimler, Mühendislik ve Teknoloji, Chemical Engineering and Technology, Chemistry, Biochemistry, Bioinorganic Chemistry, Physical Chemistry, Characterization of Polymers, Natural Sciences, Engineering and Technology, Mühendislik, Bilişim ve Teknoloji (ENG), Temel Bilimler (SCI), Mühendislik, Malzeme Bilimi, MÜHENDİSLİK, KİMYASAL, POLİMER BİLİMİ, KİMYA, ORGANİK, MALZEME BİLİMİ, ÇOKDİSİPLİNLİ, Engineering, Computing & Technology (ENG), Natural Sciences (SCI), ENGINEERING, MATERIALS SCIENCE, CHEMISTRY, ENGINEERING, CHEMICAL, POLYMER SCIENCE, CHEMISTRY, ORGANIC, MATERIALS SCIENCE, MULTIDISCIPLINARY, Genel Kimya Mühendisliği, Fizik Bilimleri, Organik Kimya, Polimerler ve Plastikler, Malzeme Kimyası, General Chemical Engineering, Physical Sciences, Organic Chemistry, Polymers and Plastics, Materials Chemistry, corneal tissue engineering, corneal ulcers, electrospinning, fluconazole, vancomycin
Citation
CESUR S., Ilhan E., Pilavci E., Sulutas R. B., Gurboga M., BİNGÖL ÖZAKPINAR Ö., Kaya E., Heljak M., TINAZ G., OKTAR F. N., et al., "A Novel Strategy as a Potential Rapid Therapy Modality in the Treatment of Corneal Ulcers: Fluconazole/Vancomycin Dual Drug-Loaded Nanofibrous Patches", Macromolecular Materials and Engineering, 2023