Publication: Effects of SPARC and possible receptors on colon cancer cell line
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Date
2023-06-01
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Abstract
Objective: The aim of this study was to observe the apoptotic/cytotoxic effects of exogenous SPARC on colon cancer cell line HT-29, then to
investigate the function of stabilin-1 and integrin αvβ3, which are possible receptors for SPARC in colon cancer cells and to determine the
quantitation of their receptor numbers.
Methods: Appropriate doses of exogenous SPARC and it’s inhibitor, cilengitide added to HT-29 cell line were determined by xCELLigence RealTime Cell Analysis system, SPARC-mediated caspase 3 expressions were measured. Using the RT-PCR system, gene expression levels of SPARC,
stabilin-1 and integrin αvβ3 receptors (silenced/nonsilenced with cilengitide) were detected then the numbers of receptors per cell were
quantitated by flow cytometry.
Results: IC50 value of SPARC was determined as 4.57 µg/mL and IC50 value of cilengitide was determined as 50 nM. 5 µg/mL exogenous
SPARC caused increased apoptosis in the HT-29 line. Significant increase in gene expression of integrin αvβ3 receptor was observed in the
group incubated with 5 µg/mL SPARC, contrarily, the addition of cilengitide decreased gene expressions. The integrin αvβ3 receptor numbers
increased approximately 2-fold with SPARC compared to the control. No significant changes were observed in the gene expression and receptor
numbers of stabilin-1.
Conclusion: Exogenous SPARC was shown to reduce proliferation and induce apoptosis in colon cancer cells. Integrin αvβ3 is thought to be the
possible receptor mediating SPARC in colon cancer cells. Quantification of surface receptors per cell, which we think we have done first, can be
considered as a marker in the follow-up of anticancer treatments.
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Tıp, Sağlık Bilimleri, Medicine, Health Sciences, Klinik Tıp (MED), Klinik Tıp, Clinical Medicine (MED), CLINICAL MEDICINE, SPARC, cilengitide, colon cancer, stabilin-1, integrin αvβ3
Citation
Mısırlı D., Bingöl Özakpınar Ö., Şekerler T., Aru B., Demirel G. Y., Tunoğlu S., Ozsavci D., "Effects of SPARC and Possible Receptors on Colon Cancer Cell Line", CLINICAL AND EXPERIMENTAL HEALTH SCIENCES, cilt.13, sa.13, ss.1-7, 2023