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The potential of archaeosomes as carriers of pDNA into mammalian cells

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dc.contributor.authorOGAN, AYŞE
dc.contributor.authorsAttar, Azade; Ogan, Ayse; Yucel, Sevil; Turan, Kadir
dc.date.accessioned2022-03-12T20:28:08Z
dc.date.available2022-03-12T20:28:08Z
dc.date.issued2016
dc.description.abstractThis paper describes the formulation of archaeosomes and the evaluation of their abilities to facilitate in vitro DNA delivery. Lipids of the H.hispanica 2TK2 strain were used in archaeosome formation, which is formulated by mixing H.hispanica 2TK2 lipids with plasmid DNA encoding green fluorescent protein (GFP) or beta-galactosidase (beta-gal). Archaeosome/pDNA formation and unbound DNA were monitored by agarose gel electrophoresis. The archaeosome formulations were visualized by AFM and TEM. The zeta potential analysis showed the archaeosomes to be electronegative. The composition of archaeosomes and the DNA dose for transient transfection into HEK293 cells were optimized, and the relationship between the structure and activity of archaeosomes in DNA delivery was investigated. By themselves, archaeosomes showed low efficiency for DNA delivery, due to their anionic nature. By formulating archaeosomes with a helper molecule, such as DOTAP, CaCl2, or LiCl, the capability of archaeosomes for gene transfection is significantly enhanced. The transfection profiles of efficient archaeosomes are proved to have a long shelf-life when maintained at room temperature. Thus, the archaeal lipids have the potential to be used as transfection reagents in vitro.
dc.identifier.doi10.3109/21691401.2014.982800
dc.identifier.eissn2169-141X
dc.identifier.issn2169-1401
dc.identifier.pubmed25650647
dc.identifier.urihttps://hdl.handle.net/11424/233855
dc.identifier.wosWOS:000370635000036
dc.language.isoeng
dc.publisherTAYLOR & FRANCIS LTD
dc.relation.ispartofARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectgene delivery
dc.subjectlipid extraction
dc.subjectarchaeosome
dc.subjectHaloarcula hispanica
dc.subjectin vitro transfection
dc.subjectORAL DELIVERY
dc.subjectULTRADEFORMABLE ARCHAEOSOMES
dc.subjectLIPOSOMES
dc.subjectLIPIDS
dc.subjectSTABILITY
dc.subjectVACCINE
dc.subjectSYSTEMS
dc.subjectBIOSYNTHESIS
dc.subjectVESICLES
dc.titleThe potential of archaeosomes as carriers of pDNA into mammalian cells
dc.typearticle
dspace.entity.typePublication
local.avesis.idd2a303c6-b4cb-44e1-b68d-89011c9f8354
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages7
local.journal.quartileQ1
oaire.citation.endPage716
oaire.citation.issue2
oaire.citation.startPage710
oaire.citation.titleARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
oaire.citation.volume44
relation.isAuthorOfPublication58db3332-a5f8-4224-a021-aeea795f52fa
relation.isAuthorOfPublication.latestForDiscovery58db3332-a5f8-4224-a021-aeea795f52fa

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