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Protein surface engineering and interaction studies of maltogenic amylase towards improved enzyme immobilisation

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dc.contributor.authorTOKSOY ÖNER, EBRU
dc.contributor.authorsJaafar N. R., Jailani N., Rahman R. A., TOKSOY ÖNER E., Murad A. M. A., Illias R. M.
dc.date.accessioned2023-07-10T13:04:39Z
dc.date.available2023-07-10T13:04:39Z
dc.date.issued2022-07-01
dc.description.abstractA combined strategy of computational, protein engineering and cross-linked enzyme aggregates (CLEAs) approaches was performed on Bacillus lehensis G1 maltogenic amylase (Mag1) to investigate the preferred amino acids and orientation of the cross-linker in constructing stable and efficient biocatalyst. From the computational analysis, Mag1 exhibited the highest binding affinity towards chitosan (-7.5 kcal/mol) and favours having interactions with aspartic acid whereas glutaraldehyde was the least favoured (-3.4 kcal/mol) and has preferences for lysine. A total of eight Mag1 variants were constructed with either Asp or Lys substitutions on different secondary structures surface. Mutant Mag1-mDh exhibited the highest recovery activity (82.3%) in comparison to other Mag1 variants. Mutants-CLEAs exhibited higher thermal stability (20-30% activity) at 80 C whilst Mag1-CLEAs could only retain 9% of activity at the same temperature. Reusability analysis revealed that mutants-CLEAs can be recovered up to 8 cycles whereas Mag1-CLEAs activity could only be retained for up to 6 cycles. Thus, it is evident that amino acids on the enzyme\"s surface play a crucial role in the construction of highly stable, efficient and recyclable CLEAs. This demonstrates the necessity to determine the preferential amino acid by the cross-linkers in advance to facilitate CLEAs immobilisation for designing efficient biocatalysts.
dc.identifier.citationJaafar N. R., Jailani N., Rahman R. A., TOKSOY ÖNER E., Murad A. M. A., Illias R. M., "Protein surface engineering and interaction studies of maltogenic amylase towards improved enzyme immobilisation", INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, cilt.213, ss.70-82, 2022
dc.identifier.doi10.1016/j.ijbiomac.2022.05.169
dc.identifier.endpage82
dc.identifier.issn0141-8130
dc.identifier.startpage70
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0141813022011540?via%3Dihub
dc.identifier.urihttps://hdl.handle.net/11424/291033
dc.identifier.volume213
dc.language.isoeng
dc.relation.ispartofINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectYaşam Bilimleri
dc.subjectMoleküler Biyoloji ve Genetik
dc.subjectSitogenetik
dc.subjectKimya
dc.subjectFizikokimya
dc.subjectPolimer Karakterizasyonu
dc.subjectDiğer
dc.subjectTemel Bilimler
dc.subjectLife Sciences
dc.subjectMolecular Biology and Genetics
dc.subjectCytogenetic
dc.subjectChemistry
dc.subjectPhysical Chemistry
dc.subjectCharacterization of Polymers
dc.subjectOther
dc.subjectNatural Sciences
dc.subjectBİYOKİMYA VE MOLEKÜLER BİYOLOJİ
dc.subjectMoleküler Biyoloji ve Genetik
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectKİMYA, UYGULAMALI
dc.subjectTemel Bilimler (SCI)
dc.subjectPOLİMER BİLİMİ
dc.subjectBIOCHEMISTRY & MOLECULAR BIOLOGY
dc.subjectMOLECULAR BIOLOGY & GENETICS
dc.subjectLife Sciences (LIFE)
dc.subjectCHEMISTRY, APPLIED
dc.subjectCHEMISTRY
dc.subjectNatural Sciences (SCI)
dc.subjectPOLYMER SCIENCE
dc.subjectBiyokimya, Genetik ve Moleküler Biyoloji (çeşitli)
dc.subjectKlinik Biyokimya
dc.subjectKanser Araştırmaları
dc.subjectMoleküler Biyoloji
dc.subjectİlaç Keşfi
dc.subjectYaşlanma
dc.subjectGenel Biyokimya, Genetik ve Moleküler Biyoloji
dc.subjectBiyokimya
dc.subjectYapısal Biyoloji
dc.subjectPolimerler ve Plastikler
dc.subjectKimya (çeşitli)
dc.subjectGenel Kimya
dc.subjectProses Kimyası ve Teknolojisi
dc.subjectFizik Bilimleri
dc.subjectBiochemistry, Genetics and Molecular Biology (miscellaneous)
dc.subjectClinical Biochemistry
dc.subjectCancer Research
dc.subjectMolecular Biology
dc.subjectDrug Discovery
dc.subjectAging
dc.subjectGeneral Biochemistry, Genetics and Molecular Biology
dc.subjectBiochemistry
dc.subjectStructural Biology
dc.subjectPolymers and Plastics
dc.subjectChemistry (miscellaneous)
dc.subjectGeneral Chemistry
dc.subjectProcess Chemistry and Technology
dc.subjectPhysical Sciences
dc.subjectComputational analysis
dc.subjectProtein engineering
dc.subjectEnzyme immobilisation
dc.subjectProtein orientation
dc.subjectProtein secondary structure
dc.subjectHETEROFUNCTIONAL CARRIER
dc.subjectCOVALENT IMMOBILIZATION
dc.subjectCANDIDA-RUGOSA
dc.subjectSTABILITY
dc.subjectLIPASE
dc.subjectORIENTATION
dc.subjectSTRATEGIES
dc.subjectSUPPORTS
dc.subjectSITE
dc.subjectBENTONITE
dc.titleProtein surface engineering and interaction studies of maltogenic amylase towards improved enzyme immobilisation
dc.typearticle
dspace.entity.typePublication
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local.indexed.atWOS
local.indexed.atPUBMED
local.indexed.atSCOPUS
relation.isAuthorOfPublication6118e9e4-a58e-429b-bbec-4f73a3089a2b
relation.isAuthorOfPublication.latestForDiscovery6118e9e4-a58e-429b-bbec-4f73a3089a2b

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