Publication:
Biochemical characterization of avian influenza viral polymerase containing PA or PB2 subunit from human influenza A virus

Simple item page
dc.contributor.authorTURAN, KADİR
dc.contributor.authorsPhu Tran Vinh Pham; Turan, Kadir; Nagata, Kyosuke; Kawaguchi, Atsushi
dc.date.accessioned2022-03-12T22:25:32Z
dc.date.available2022-03-12T22:25:32Z
dc.date.issued2018
dc.description.abstractAdaptive mutations in viral polymerase, which is composed of PB1, PB2, and PA, of avian influenza viruses are major genetic determinants of the host range. In this study, to elucidate the molecular mechanism of mammalian adaptation of avian viral polymerase, we performed cell-based vRNP reconstitution assays and biochemical analyses using purified recombinant viral polymerase complexes. We found that avian viral polymerase from A/duck/Pennsylvania/10,218/84 (DkPen) enhances the viral polymerase activity in mammalian cells by replacing the PA or PB2 gene with that from human influenza virus A/WSN/33 (WSN). Chimeric constructs between DkPen PA and WSN PA showed that the N-terminal endonuclease domain of WSN PA was essential for the mammalian adaptation of DkPen viral polymerase. We also found that the cap-snatching activity of purified DkPen viral polymerase was more than 5 times weaker than that of WSN in vitro in a PB2 Glu627-dependent manner. However, the cap-snatching activity of DkPen viral polymerase was hardly increased by replacing DkPen PA to WSN PA. These results suggest that the activity of viral genome replication may be enhanced in the DkPen reassortant containing WSN PA. (C) 2018 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
dc.identifier.doi10.1016/j.micinf.2018.04.003
dc.identifier.eissn1769-714X
dc.identifier.issn1286-4579
dc.identifier.pubmed29729434
dc.identifier.urihttps://hdl.handle.net/11424/234937
dc.identifier.wosWOS:000437620400006
dc.language.isoeng
dc.publisherELSEVIER SCIENCE BV
dc.relation.ispartofMICROBES AND INFECTION
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCap-snatching
dc.subjectEndonuclease
dc.subjectMammalian adaption
dc.subjectReassortment
dc.subjectViral polymerase
dc.subjectADAPTIVE MUTATIONS
dc.subjectHOST RESTRICTION
dc.subjectRNA-POLYMERASE
dc.subjectINCREASED VIRULENCE
dc.subjectAMINO-ACID
dc.subjectMICE
dc.subjectPROTEIN
dc.subjectENDONUCLEASE
dc.subjectADAPTATION
dc.subjectREPLICATION
dc.titleBiochemical characterization of avian influenza viral polymerase containing PA or PB2 subunit from human influenza A virus
dc.typearticle
dspace.entity.typePublication
local.avesis.id83802565-4766-41c8-a928-167c4cbbcf06
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages7
local.journal.quartileQ3
oaire.citation.endPage359
oaire.citation.issue6
oaire.citation.startPage353
oaire.citation.titleMICROBES AND INFECTION
oaire.citation.volume20
relation.isAuthorOfPublication9cc23fc2-5031-46cd-91a0-a2e13ab788d4
relation.isAuthorOfPublication.latestForDiscovery9cc23fc2-5031-46cd-91a0-a2e13ab788d4

Files

Collections

Research Outputs