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Development of a cost-effective production process for Halomonas levan

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dc.contributor.authorTOKSOY ÖNER, EBRU
dc.contributor.authorsErkorkmaz, Burak Adnan; Kirtel, Onur; Duru, Ozlem Ates; Oner, Ebru Toksoy
dc.date.accessioned2022-03-12T22:25:57Z
dc.date.available2022-03-12T22:25:57Z
dc.date.issued2018
dc.description.abstractLevan polysaccharide is an industrially important natural polymer with unique properties and diverse high-value applications. However, current bottlenecks associated with its large-scale production need to be overcome by innovative approaches leading to economically viable processes. Besides many mesophilic levan producers, halophilic Halomonas smyrnensis cultures hold distinctive industrial potential and, for the first time with this study, the advantage of halophilicity is used and conditions for non-sterile levan production were optimized. Levan productivity of Halomonas cultures in medium containing industrial sucrose from sugar beet and food industry by-product syrup, a total of ten sea, lake and rock salt samples from four natural salterns, as well as three different industrial-grade boron compounds were compared and the most suitable low-cost substitutes for sucrose, salt and boron were specified. Then, the effects of pH control, non-sterile conditions and different bioreactor modes (batch and fed-batch) were investigated. The development of a cost-effective production process was achieved with the highest yield (18.06 g/L) reported so far on this microbial system, as well as the highest theoretical bioconversion efficiency ever reported for levan-producing suspension cultures. Structural integrity and biocompatibility of the final product were also verified in vitro.
dc.identifier.doi10.1007/s00449-018-1952-x
dc.identifier.eissn1615-7605
dc.identifier.issn1615-7591
dc.identifier.pubmed29774415
dc.identifier.urihttps://hdl.handle.net/11424/234994
dc.identifier.wosWOS:000441931100002
dc.language.isoeng
dc.publisherSPRINGER
dc.relation.ispartofBIOPROCESS AND BIOSYSTEMS ENGINEERING
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectExopolysaccharide
dc.subjectLevan
dc.subjectHalomonas smyrnensis
dc.subjectMicrobial bioprocess
dc.subjectCost-effective production
dc.subjectSMYRNENSIS AAD6(T)
dc.subjectZYMOMONAS-MOBILIS
dc.subjectMICROBIAL LEVAN
dc.subjectBIOMEDICAL APPLICATIONS
dc.subjectBACILLUS-SUBTILIS
dc.subjectMOLECULAR-WEIGHT
dc.subjectMULTILAYER FILMS
dc.subjectSULFATED LEVAN
dc.subjectTRACE-METALS
dc.subjectLEVANSUCRASE
dc.titleDevelopment of a cost-effective production process for Halomonas levan
dc.typearticle
dspace.entity.typePublication
local.avesis.id9cccf4f8-b1f1-4c50-a182-0df302418c07
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages13
local.journal.quartileQ2
oaire.citation.endPage1259
oaire.citation.issue9
oaire.citation.startPage1247
oaire.citation.titleBIOPROCESS AND BIOSYSTEMS ENGINEERING
oaire.citation.volume41
relation.isAuthorOfPublication6118e9e4-a58e-429b-bbec-4f73a3089a2b
relation.isAuthorOfPublication.latestForDiscovery6118e9e4-a58e-429b-bbec-4f73a3089a2b

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