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GÖKALSIN, BARIŞ

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GÖKALSIN

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BARIŞ

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Now showing 1 - 3 of 3
  • PublicationOpen Access
    Using proteomics, q-PCR and biochemical methods complementing as a multiapproach to elicit the crucial responses of zebrafish liver exposed to neonicotinoid pesticide
    (2023-06-01) SESAL, NÜZHET CENK; GÖKALSIN, BARIŞ; KAYHAN, FİGEN ESİN; Kızılkaya Ş., Akpınar G., Sesal N. C., Kasap M., Gökalsın B., Kayhan F. E.
    Pesticides enter the environment through runoff and leaching and this raises public concern about effects on non-target organisms. Imidacloprid (IMI) a synthetic pesticide, has an unstable half-life, metabolized in minutes to weeks in the water. To evaluate the effects of IMI on the zebrafish liver, we conducted proteomic, molecular and biochemical analysis in a multi-level approach, to highlight the complementary features regarding the results of each method. Adult zebrafish were exposed to 60 mg/L IMI for 48 hours and were evaluated using nLC-MS/MS for proteins, q-PCR analysis for expression of cat, gpx, pxr, ache, along with CAT and AChE enzyme activities and GSH and MDA assays. Based on proteomics, the regulation of antioxidant and immune responses, as well as gene transcription were significant processes affected. Apoptosis and ER stress pathways were upregulated and there was a down-regulation of cat and gpx genes. There was also elevated CAT activity and GSH and decreased MDA. Additionally, elevated AChE activity and up regulation of ache expression was observed. The multi-approach results included regulators of antioxidant, xenobiotic response and neuro-protective related proteins (genes and enzymes), which overall reflected harmful effects of IMI. Consequently, this study highlights the effects of IMI on zebrafish liver and reveals new potential biomarkers. In this respect, evaluated outcomes reveal the complementary features emphasizing the importance of studying chemicals using several methods. Our study provides deeper insights for future work in ecotoxicological studies regarding IMI and contribute to existing toxicity literature.
  • PublicationOpen Access
    Pseudomonas aeruginosa quorum sensing and biofilm inhibition
    (Academic Press, 2019-01-01) GÖKALSIN, BARIŞ; SESAL, NÜZHET CENK; GÖKALSIN B., BERBER D., SESAL N. C.
  • PublicationOpen Access
    Effects of ultrasonic specific energy on time-dependent inactivation rate of proteus mirabilis and streptococcus sanguinis
    (2023-01-01) GÖKALSIN, BARIŞ; SESAL, NÜZHET CENK; Kekeç Ö., GÖKALSIN B., Açıkgöz B., Özdaş D. Ö., Sesal C.
    Ultrasound has been used as an alternative and enhancer approach for the inhibition of microorganisms in recent years. The purpose of the current study was to determine the inactivation of Streptococcus sanguinis and Proteus mirabilis bacteria species via ultrasound. For this purpose, bacterial suspensions with 5x103, 1.5x104, and 3x104 colony forming unit (cfu/ml) concentrations were ultra-sonicated with 30 kHz frequency and 100 W power ultrasound for various sonication time periods. As a result, time-dependent inhibition rates were observed with bacteria concentration differences. The specific energy (γ) of different concentrations of S. sanguinis and P. mirabilis was also calculated. The rate constant of ultrasonic inhibition was evaluated in the linear region against sonication time. Then, the t-test was applied to all of the data. The results of 5x103, 1.5x104, and 3x104 cfu/ml for S. sanguinis and P. mirabilis were statistically significant at