Publication:
High frequency somatic embryogenesis in cotton

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dc.contributor.authorsAydin, Y; Ipekci, Z; Talas-Ogras, T; Zehir, A; Bajrovic, K; Gozukirmizi, N
dc.date.accessioned2022-03-12T17:17:40Z
dc.date.available2022-03-12T17:17:40Z
dc.date.issued2004
dc.description.abstractA highly reproducible system for efficient somatic embryogenesis was developed to regenerate plantlets from cotton ( Gossypium hirsutum L.) cultivars (Nazilli M-503 and Nazilli 143). Shoot apices, hypocotyls and nodes of 10-d-old seedlings were used as explants. High frequency (100%) embryogenic calli was initiated from all tested explants on Murashige and Skoog (1962) (MS) media supplemented with 1 g dm(-3) polyvinylpyrrolidone (PVP), 1 mg dm(-3) 6-benzylaminopurine (BAP), 0.5 mg dm(-3) kinetin for Nazilli M-503 and 1 g dm(-3) PVP, 2 mg dm(-3) BAP, 0.5 mg dm(-3) kinetin for Nazilli-143. Globular stage somatic embryos were produced 4 months after transfer to hormone-free MS medium supplemented with 1 g dm(-3) PVP. Subsequent subculture of globular embryos every 3 weeks on hormone-free MS medium led to the development of torpedo and cotyledonary stage embryos with the frequency of 75 and 83.2% from hypocotyl explants of Nazilli M-503 and Nazilli-143, respectively. Afterwards, mature somatic embryos were isolated and cultured on hormone-free MS medium for germination and development into plantlets. The highest germination frequency (42.9%) for Nazilli M-503 somatic embryos were obtained on hormone-free MS medium after 5 months with hypocotyl explants, whereas germination frequencies of Nazilli-143 embryos from hypocotyl, node and apex explants varied between 22 - 30%.
dc.identifier.doi10.1023/B:BIOP.0000047142.07987.e1
dc.identifier.eissn1573-8264
dc.identifier.issn0006-3134
dc.identifier.urihttps://hdl.handle.net/11424/227884
dc.identifier.wosWOS:000224910600003
dc.language.isoeng
dc.publisherACAD SCIENCES CZECH REPUBLIC, INST EXPERIMENTAL BOTANY
dc.relation.ispartofBIOLOGIA PLANTARUM
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectGossypium hirsutum L.
dc.subjectindirect somatic embryogenesis
dc.subjecthypocotyls
dc.subjectcotyledonary node
dc.subjectapex
dc.subjectGOSSYPIUM-HIRSUTUM-L
dc.subjectPLANT-REGENERATION
dc.subjectSUSPENSION CULTURES
dc.subjectTISSUE
dc.subjectINDUCTION
dc.subjectGENOTYPE
dc.subjectLEAVES
dc.subjectCULTIVARS
dc.subjectEXPLANTS
dc.subjectVARIETY
dc.titleHigh frequency somatic embryogenesis in cotton
dc.typearticle
dspace.entity.typePublication
local.avesis.idc653f7bd-8ddf-4c2b-936d-546f879b77cb
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.journal.numberofpages5
oaire.citation.endPage495
oaire.citation.issue4
oaire.citation.startPage491
oaire.citation.titleBIOLOGIA PLANTARUM
oaire.citation.volume48

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