ŞENER, AZİZEÖZSAVCI, DERYAOBA, RABİA2022-03-142022-03-142010-06-011300-7777https://hdl.handle.net/11424/243614Objective: We aimed to detect novel in vitro effects of clopidogrel on platelets by assessment of the following parameters: malondialdehyde, glutathione, nitrite, aggregation response, and expressions of P-selectin, fibrinogen, apolipoprotein A1, apolipoprotein B, and phosphatidylserine. Materials and Methods: Platelets were obtained from healthy (n: 9) and hyperlipidemic (n: 9) volunteers. Expressions of P-selectin, fibrinogen, apolipoproteins A1/B and phosphatidylserine with and without clopidogrel were assayed by flow cytometry. Malondialdehyde, glutathione, aggregation and nitrite levels were also assayed. Results: Without clopidogrel, the baseline values of platelet aggregation, malondialdehyde, and expressions of P-selectin, fibrinogen and phosphatidylserine were significantly higher, whereas nitrite and expression of apolipoproteins A1/B were significantly lower in hyperlipidemics than in the healthy group. In both groups, clopidogrel significantly reduced aggregation and expression of fibrinogen, but it elevated nitrite levels. Clopidogrel significantly decreased P-selectin and phosphatidylserine expression and malondialdehyde but increased expressions of apolipoproteins A1/B only in hyperlipidemics. Conclusion: It seems that clopidogrel has some new in vitro antiplatelet effects. The present study is a basic in vitro study to suggest new insights into the effects of clopidogrel on platelet functions. (Turk J Hematol 2010; 27: 99-108)enginfo:eu-repo/semantics/openAccessPlateletshyperlipidemiaclopidogrelapolipoprotein A1apolipoprotein Boxidative stressnitritesphosphatidylserineP-selectinglycoproteins IIb/IIIaLOW-DENSITY-LIPOPROTEINGLYCOPROTEIN IIB/IIIAP-SELECTINNORMOLIPEMIC VOLUNTEERSSECONDARY PREVENTIONACTIVATED PLATELETSBINDINGINHIBITIONEXPRESSIONAPOPTOSISarticleWOS:00027894750000710.5152/tjh.2010.07272634511308-5263