Browsing by Author "ERCAN, FERİHA"
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Publication Metadata only 2-mercaptoethane sulfonate (MESNA) protects against biliary obstruction-induced oxidative damage in rats(ELSEVIER IRELAND LTD, 2006) ERCAN, FERİHA; Sener, G; Kabasakal, L; Sehirli, O; Ercan, F; Gedik, NThe aim of this study was to assess the antioxidant and antifibrotic effects of chronic administration of 2-mercaptoethane sulfonate (MESNA) on oxidative liver damage and fibrosis induced by biliary obstruction in rats. Liver fibrosis was induced in male Wistar albino rats by bile duct ligation and scission (BDL). MESNA (150 mg/kg, i.p.) or saline was administered for 28 days. At the end of the experiment, rats were killed by decapitation. Serum aspartate aminotransferase (AST), and alanine aminotransferase (ALT) levels were determined to assess liver function. Tumor necrosis factor-alpha (TNF-alpha) and lactate dehidrogenase (LDH) were also assayed in serum samples. Liver tissues were taken for determination of the free radicals, hepatic malondialdehyde (MDA) levels, an end product of lipid peroxidation; glutathione (GSH) levels, a key antioxidant; myeloperoxidase (MPO) activity, as an indirect index of neutrophil infiltration. Hepatic collagen content, as a fibrosis marker was also determined. Serum AST, ALT, LDH and TNF-alpha levels were elevated in the BDL group as compared to control group, while this increase was significantly decreased by MESNA treatment. BDL caused a significant (p < 0.05-0.001) decrease in GSH levels while MDA levels and MPO activity were increased in the liver tissue. These changes were reversed by MESNA treatment. Collagen contents of the liver tissue was increased by BDL (P < 0.001), and reversed back to the control levels with MESNA. Since MESNA administration alleviated the BDL-induced oxidative injury of the liver and improved the hepatic functions, it seems likely that MESNA with its antioxidant and antifibrotic properties, may be of potential therapeutic value in protecting the liver fibrosis and oxidative injury due to biliary obstruction. (c) 2006 Published by Elsevier Ireland Ltd.Publication Metadata only 83: phoenixin-14 ameliorates cholestatic liver injury and bileinduced acute pancreatic injury in rats(2022-05-01) KAHRAMAN, MERVE MERİÇ; YÜKSEL, MERAL; YEGEN, BERRAK; ERCAN, FERİHA; Şen L. S. , Kahraman M. M. , Mermer K. S. , Köroğlu K., Yüksel M., İmeryüz N., Ercan F., Yegen B.Background: Bile duct obstruction, which results in cholestatic liver injury, is also the major cause of acute pancreatitis. Phoenixin (PNX) was originally defined as a hypothalamic peptide associated with a wide range of physiological processes and exerts antioxidant and antiinflammatory effects. PNX is expressed in several peripheral organs including pancreas and liver. We aimed to evaluate possible therapeutic effects of PNX on hepatic and pancreatic damage induced by biliary or pancreaticobiliary duct obstruction. Methods: In male Sprague Dawley rats, bile duct ligation (BDL; n=16) or pancreaticobiliary duct ligation (PBDL; n= 16) was performed under ketamine anesthesia, while control rats (n=8) had sham-surgery. Either PNX-14 (50 µg/kg/day) or saline was subcutaneously injected immediately after surgery and in the following 2 days. On the post-operative 3rd day, hepatic and renal blood flow was measured using laser Doppler flowmeter under anesthesia and the rats were then euthanized. In the liver and pancreas samples, levels of malondialdehyde, antioxidant glutathione and myeloperoxidase activity were measured by spectrophotometry, while luminol- and lucigenin-enhanced chemiluminescence (CL) levels were measured to assess formation of reactive oxygen species (ROS). Tissue samples were stained by hematoxylin-eosin to calculate microscopic damage scores. Statistical analyses were made by one-way ANOVA. Results: Increased microscopic damage scores in the pancreas of saline-treated PBDL (p<0.001) and in the liver of saline-treated BDL group (p<0.001) were reduced by PNX14 treatment (p<0.01), but PBDL-induced hepatic damage (p<0.001) was not changed by PNX-14. Pancreatic and hepatic levels of malondialdehyde and myeloperoxidase activity and pancreatic glutathione levels were not different among the experimental groups, while hepatic glutathione level was elevated in PNX-14-treated BDL (p<0.001) and PBDL (p<0.01) groups as compared to control group. Despite a non-significant fall in renal or hepatic blood flow in saline-treated BDL rats, PBDL significantly reduced hepatic (p<0.001) and renal (p<0.01) blood flow, while PNX-14 reversed blood flow in both organs back to control levels (p<0.05). The CL levels of luminol and lucigenin were increased in both hepatic and pancreatic tissues of saline-treated BDL and PBDL groups (p<0.05-0.001), showing enhanced ROS generation. However, CL levels in both the liver and pancreas of PNX-14-treated BDL and PBDL groups were significantly reduced as compared to those measured in the liver and pancreas of respective saline-treated groups (p<0.05-0.001). Conclusion: In conclusion, cholestatic liver injury and bile-induced pancreatic injury are alleviated by PNX-14 treatment, which appears to act via its ROS scavenging activity, by replenishing hepatic antioxidant capacity and restoring impaired organ perfusion.Publication Metadata only A comparative ontogenic study of urinary bladder: Impact of the epithelial differentiation in embryonic and newborn rats(WILEY, 2006) ERCAN, FERİHA; Ersoy, Y.; Ercan, F.; Cetinel, S.The present study aimed to show the cellular and subcellular distribution of glycogen content during the differentiation of urothelial cells from simple cuboidal to stratified transitional epithelium. Bladder samples were taken from rat embryos on the 15th to 19th days and newborn at 21st day. During the development of the bladder, the formation of fusiform vesicles, asymmetric unit membrane (AUM) and microridges were examined with staining with haematoxylin-eosin and periodic acid Schiff for light microscope and periodic acid-thiocharbohydrazide-silver proteinate for transmission electron microscope. The topographical changes of luminal differentiation were examined with the scanning electron microscope. The urothelium was simple cuboidal from 15th till the 17th days of gestation. Glycogen content was present in the cytoplasm till the 18th day of gestation. At the early stage (16th day) of gestation, the apical surface contains microvilli that points the undifferentiated cells. The density of microvilli decreased and ropy microridges appeared at the 17th day of gestation. The small discoid vesicles lined with AUM developed at the apical cytoplasm of the surface cells at the 17th day of gestation. After this stage, both the density of microridges and large and elongated fusiform vesicles increased. The differentiation of the urothelium begins with the formation of the round and small vesicles, continues with the formation of the AUM and at the final stage there is a decrease in both glycogen content and the appearance of the microridges at the luminal surface of the urothelial cells.Publication Open Access A Histological Evaluation of the Effect of Ghrelin on Wound Healing in Rats(2022-12-01) AK, ESİN; ERCAN, FERİHA; AK E., ULUSOY-DAĞ K., ERCAN F., ÇORAK A.Objective: This study aimed to investigate the potential effects of ghrelin on wound healing. Materials and Methods: Sprague-Dawley rats were divided into 3 groups: Control (n = 8), wound-saline (W+S, n = 16), and wound-ghrelin (W+Gr, n = 16). A wound was created on the cervical back region of rats using an 8 mm biopsy punch tool in the W+S and W+Gr groups. Either saline (1 mL/kg) or ghrelin (10 ng/kg) was administrated intraperitoneally each day to the rats in the non-control groups after the onset of the wound. Rats from the W+S and W+Gr groups were euthanized on the 7th (n = 8 from each group) and 14th day (n = 8 from each group) of the experiment. The histopathological score was evaluated statistically using one-way analysis of variance and Tukey’s multiple comparison tests. Results: The rats euthanized from the W+S group on day 7 (subgroup W+S7) showed degenerated epidermis, no hair follicles, presence of granulation tissue, inflammatory cell infiltration, vasocongestion, and increased collagen fibers in dermis. However, all these histopathological findings significantly decreased in the rats euthanized from the W+Gr group on day 7 (subgroup W+Gr7) compared to the W+S7 group (p < 0.05). The W+S14 group showed thick epidermis, a few hair follicles, angiogenesis, and increased collagen fibers in the dermis. Additionally, the histopathological findings decreased significantly in the W+Gr14 group compared to W+S14 group (p < 0.05). Conclusion: Based on the statistical analysis of the histological findings, the ghrelin treatment appears to have a beneficial effect on wound healing. Keywords: Wound healing, ghrelin and histologyPublication Metadata only A preliminary study, using electron and light-microscopic methods, of axon numbers in the fornix in autopsies of patients with temporal lobe epilepsy(SPRINGER, 2009) ERCAN, FERİHA; Ozdogmus, Omer; Cavdar, Safiye; Ersoy, Yasemin; Ercan, Feriha; Uzun, IbrahimOur objective was to show morphological changes of the fornix in autopsies of patients with temporal lobe epilepsy, which may potentially serve for post-mortem diagnosis. Epileptic and non-epileptic autopsy brains were obtained from the council of forensic medicine between the years 2005 and 2007. In both non-epileptic and epileptic autopsies the mean cross-sectional areas and fiber densities of the right and left fornices were calculated and analyzed. The numbers of myelinated and unmyelinated fibers, and the total number of fibers forming each fornix were counted. The total number of fibers in the right fornix was always greater than in the left fornix, in both epileptic and non-epileptic autopsies. The mean total number of fornix fibers was significantly reduced in epileptics compared with non-epileptics, in both the right (p = 0.043) and left (p = 0.043) sides. The electron-microscopic sections showed that myelinated axons outnumbered unmyelinated axons in both epileptic and non-epileptic autopsies. However, the reduction in the number of unmyelinated fibers was only statistically significant for the right fornix in right epileptic autopsies (p = 0.021). Although the reduction in the number of myelinated fibers was not statistically significant, electron-microscopic evaluations showed myelin degeneration of the myelinated fibers in the right fornix of the right temporal lobe in epileptic autopsies. In conclusion, our results suggest that unmyelinated fiber loss is functionally important, and may have functional consequences of diagnostic value.Publication Metadata only Acute lung injury following thermal insult to the skin: a light and transmission electron microscopial study(1999) ERCAN, FERİHA; Arbak, S.; Ercan, F.; Hürdağ, C. G.; Karabulut, O.; Gürbüz, V.; Corak, A.; Alican, I.Oxygen radicals are involved in the development of burn shock and distant organ injury in animal models of trauma. Neutrophils are likely the source of reactive oxygen metabolites as a result of the systemic inflammatory reaction to a local burn insult. The aim of the present study was to assess the role of neutrophils in the development of lung injury related to second degree skin burn in rats. Rats were decapitated at two hours following burn injury. Lung tissue samples were removed and examined biochemically and histologically. Tissue-associated myeloperoxidase (MPO) activity, which is an index of neutrophil infiltration, was increased considerably in lung tissue at 2 h after burn injury. Disturbance of alveolar structure, intraalveolar hemorrhage and prominent neutrophil infiltration indicated lung parenchymal injury. Ultrastructural examination of the lung revealed that pneumocytes type I, pneumocytes type II and capillary endothelial cells were degenerated. The data presented here suggest that neutrophil accumulation in the lung is involved in pathogenesis of this distant organ after burn injury.Publication Metadata only Alpha-Lipoic Acid Improves Acetic Acid-Induced Gastric Ulcer Healing in Rats(SPRINGER/PLENUM PUBLISHERS, 2009) YEGEN, BERRAK; Karakoyun, Berna; Yuksel, Meral; Ercan, Feriha; Erzik, Can; Yegen, Berrak C.To evaluate the role of ALA treatment on the healing of acetic acid-induced gastric ulcer, rats were given ALA (35 mg/kg/day) or saline for 3 days before the induction of ulcer and the treatment was continued twice daily for 2 days (early) or 10 days (late) until they were decapitated. Gastric ulcer index, microscopic score, elevated DNA fragmentation and chemiluminescence levels of the saline-treated ulcer groups were all reduced by ALA treatment. Likewise, ALA treatment inhibited chemiluminescence levels in both early and late ulcer groups. Marked reduction in glutathione levels of the saline-treated early ulcer group was reversed by ALA treatment, while ALA treatment was effective in depressing gastric myeloperoxidase activity in the late ulcer group. In conclusion, ALA treatment shows protective role in the healing of acetic acid-induced gastric injury in rats via the suppression of neutrophil accumulation, preservation of endogenous glutathione, inhibition of reactive oxidant generation and apoptosis.Publication Metadata only alpha-Lipoic acid modulates gut inflammation induced by trinitrobenzene sulfonic acid in rats(WILEY, 2007) ERCAN, FERİHA; Kolgazi, Meltem; Jahovic, Nermina; Yueksel, Meral; Ercan, Feriha; Alican, InciBackground and Aim: alpha-Lipoic acid (ALA) has been shown to combat oxidative stress by quenching a variety of reactive oxygen species. It is involved in the regeneration of exogenous and endogenous antioxidants, chelation of metal ions, and repair of oxidized proteins. This study aimed to evaluate the potential beneficial effect of ALA on trinitrobenzenesulfonic acid (TNBS)-induced gut ileitis and colitis in rats. Method: After 48 h of fasting, Sprague-Dawley rats underwent a laparotomy under ether anesthesia. TNBS solution 30 mg/mL in 40% ethanol (1 mL) was injected into the lumen, 10 cm proximal to the ileocolonic junction to induce ileitis or intrarectally 8 cm proximal to the anal sphincter to induce colitis. ALA (25 mg/kg intraperitoneally, twice a day) was given after induction of inflammation and continued for 3 days. All animals were decapitated 3 days after induction of the inflammation. The mucosal lesions of the ileum and colon were scored macroscopically and microscopically. Samples were taken for the measurement of malondialdehyde (MDA) and glutathione (GSH) levels, tissue-associated myeloperoxidase (MPO) activity and luminol- or lucigenin-enhanced chemiluminescence (CL). Results: Macroscopic scores, morphological changes and increased tissue lipid peroxidation with a concomitant reduction in GSH of the ileitis or colitis groups were all reversed by treatment with ALA. ALA treatment was also effective in improving tissue MPO activity and CL values, which were elevated in untreated ileitis or colitis groups. Conclusion: ALA is beneficial in TNBS-induced gut inflammation in rats via suppression of neutrophil accumulation, preservation of endogenous glutathione and inhibition of reactive oxidant generation.Publication Metadata only Alpha-lipoic acid protects against hepatic ischemia-reperfusion injury in rats(KARGER, 2007) DULUNDU, ENDER; Dulundu, Ender; Ozel, Yahya; Topaloglu, Umit; Sehirli, Ozer; Ercan, Feriha; Gedik, Nursal; Sener, GokselBackground and Aim: To evaluate the protective effect of alpha-lipoic acid in reducing oxidative damage after severe hepatic ischemia/reperfusion (IR) injury. Methods: Wistar albino rats were subjected to 45 min of hepatic ischemia, followed by 60 min reperfusion period. Lipoic acid (100 mg/kg i.p.) was administered 15 min prior to ischemia and immediately before reperfusion period. At the end of the reperfusion period aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH) activity, and cytokine, TNF-alpha and IL-1 beta levels were determined in serum samples. Malondialdehyde (MDA), and glutathione (GSH) levels and myeloperoxidase (MPO) activity were determined in the liver tissue samples while formation of reactive oxygen species was monitored by using chemiluminescence ( CL) technique with luminol and lucigenin probes. Tissues were also analyzed histologically. Results: Serum ALT, AST, and LDH activities and TNF-alpha and IL-1 beta levels were elevated in the I/R group, while this increase was significantly lower in the group of animals treated concomitantly with lipoic acid. Hepatic GSH levels, significantly depressed by I/R, were elevated back to control levels in lipoic acid-treated I/R group. Furthermore, increases in tissue luminol and lucigen-in CL, MDA levels and MPO activity due to I/R injury were reduced back to control levels with lipoic acid treatment. Conclusion: Since lipoic acid administration alleviated the I/R-induced liver injury and improved the hepatic structure and function, it seems likely that lipoic acid with its antioxidant and oxidant-scavenging properties may be of potential therapeutic value in protecting the liver against oxidative injury due to ischemia-reperfusion. Copyright (c) 2007 S. Karger AG, Basel.Publication Open Access Ameliorating effects of exercise on disrupted epididymal sperm parameters in high fat diet-induced obese rats(MARMARA UNIV, FAC MEDICINE, 2019-01-31) ERCAN, FERİHA; Acikel Elmas, Merve; Arbak, Serap; Ercan, FerihaObjective: Obesity causes male infertility problems and affects the sperm quality. Recent studies have shown that exercise has positive effects on male fertility. The present study aimed to show the effects of swimming exercise on the epididymal sperm number, motility and morphology in hight fat diet (HFD)-induced obese rats. Materials and Methods: Four experimental groups (n=8 in each group) were formed. Standard (STD) and STD+Exercise (STD+EXC) groups were fed with standard rat diet (6% of calories as fat); HFD and HFD+Exercise (HFD+EXC) groups were fed with high fat diet (45% of calories as fat) for 18 weeks. The rats in STD+EXC and HFD+EXC groups were trained by swimming sessions (1 h per day for 5 days a week) during the last 6 weeks of the experiment. The left caudal epididymis was prepared to evaluate the number, motility and morphology of the spermatozoa. The right epididymal samples were processed for histological evaluation. Results: Normospermic parameters were seen in STD and STD+EXC groups. Sperm number and motility decreased and spermatozoa with abnormal morphology increased significantly in HFD group when compared with STD group. A large number of spermatozoa in the epididymal duct lumen and regular morphology of the fibromuscular connective tissue were observed in STD and STD+EXC groups. Most of the epididymal ducts consisted of decreased amount of spermatozoal accumulation in the HFD group. Degenerated pseudostratified columnar epithelium with vacuole formation were additional findings in this group. On the other hand, swimming exercise had an enhancement effect on sperm parameters with prominent spermatozoal accumulation in the ducts of epididymis in HFD induced obese rats. Conclusion: This study shows that HFD-induced obesity decreased the number and motility of spermatozoa, increased abnormal spermatozoa and caused disrupted epididymal morphology. We hypothesize that exercise enhanced HFD-induced spermatogenic and epididymal damages by the regulation of scrotal heat and possible inhibition of oxidative damage in the epididymis.Publication Metadata only Amelioration of sepsis-induced hepatic and ileal injury in rats by the leukotriene receptor blocker montelukast(CHURCHILL LIVINGSTONE, 2005) YEGEN, BERRAK; Sener, G; Sehirli, O; Cetinel, S; Ercan, F; Yuksel, M; Gedik, N; Yegen, BCBackground. Sepsis is a generalized inflammatory response, which involves organ systems remote from the locus of the initial infectious insult, involves the release of cytokines and the subsequent formation of reactive oxygen and nitrogen species. Objective: The aim of this study was to investigate the possible protective effect of montelukast, a leukotriene receptor blocker, against oxidative damage in the liver and ileum of septic rats. Methods: Sepsis was induced by cecal ligation and puncture method in female Wistar albino rats. Sepsis and sham operated (control) groups received either saline or montelukast (10 mg/kg, ip) immediately after the operation and at 12 h. Twenty-four hours after the surgery, rats were decapitated and malondialdehyde (MDA) content-an index of lipid peroxidation, glutathione (GSH) levels-a key antioxidant, myeloperoxidase (MPO) activity-an index of neutrophil infiltration, and collagen contents were determined in the liver and ileum. Formation of reactive oxygen species in liver and ileal tissue samples was monitored by using chemiluminescence (CL) technique with luminol and lucigenin probes. Both tissues were also analyzed histologically. Serum lactate dehydrogenase (LDH) and tumor necrosis factor-alpha (TNF-alpha) level were assessed in trunk blood. Results: Sepsis resulted in decreased GSH levels, and increased MDA levels, MPO activity, CL levels and collagen contents in both the liver and the ileum (P < 0.05 - P < 0.001) indicating the presence of the oxidative damage. Similarly, serum TNF-alpha and LDH were elevated in the sepsis group as compared to control group. On the other hand, montelukast treatment reversed all these biochemical indices, as well as histopathological alterations, which were induced by sepsis. Conclusion: Findings of the present study suggest that montelukast possesses an anti-inflammatory effect on sepsis-induced hepatic and intestinal damage and protects against oxidative injury by a neutrophil-dependent mechanism. (C) 2005 Elsevier Ltd. All rights reserved.Publication Metadata only Anti-inflammatory effects of leptin and cholecystokinin on acetic acid-induced colitis in rats: role of capsaicin-sensitive vagal afferent fibers(2003) YEGEN, BERRAK; Bozkurt, Ayhan; Cakir, Bariş; Ercan, Feriha; Yeğen, Berrak C.Leptin and cholecystokinin (CCK) have a synergistic interaction in the suppression of food intake, and afford similar gastroprotective activity. The present study was designed to investigate the putative protective effects of CCK and leptin on acute colonic inflammation. Leptin or CCK-8s was injected to rats intraperitoneally immediately before and 6 h after the induction of colitis with acetic acid. CCK-A receptor antagonist (L-364,718) or CCK-B receptor antagonist (L-365,260) was injected intraperitoneally 15 min before leptin or CCK treatments. In a group of rats, vagal afferent fibers were denervated by topical application of capsaicin on the cervical vagi. Rats were decapitated at 24 h, and the distal 8 cm of the colon were removed for macroscopic scoring, determination of tissue wet weight index (WWI), histologic assessment and tissue myeloperoxidase (MPO) activity. All inflammation parameters were increased by acetic acid-induced colitis compared to control group. Leptin or CCK-8s treatment reduced these parameters in a similar manner, while co-administration of leptin and CCK was found to be more effective in reducing the macroscopic score and WWI. CCK-8s-induced reduction in the score and WWI was prevented by CCK-A, but not by CCK-B receptor antagonist, whereas neither antagonist altered the inhibitory effect of leptin on colitis-induced injury. On the other hand, perivagal capsaicin prevented the protective effects of both CCK-8s and leptin on colitis. Our results indicate that leptin and CCK have anti-inflammatory effects on acetic acid-induced colitis in rats, which appear to be mediated by capsaicin-sensitive vagal afferent fibers involving the reduction in colonic neutrophil infiltration.Publication Open Access Anti-inflammatory effects of montelukast on smoke-induced lung injury in rats(NOVAMEDIA, 2010) ERCAN, FERİHA; Basyigit, Ilknur; Sahin, Murat; Sahin, Deniz; Yildiz, Fusun; Boyaci, Hasim; Sirvanci, Serap; Ercan, FerihaAim: To evaluate the effects of montelukast in smoke-induced lung injury. Methods: 28 Wistar-Albino rats were enrolled into 4 groups with 7 rats per group. The healthy control group was exposed to fresh air while all rats in the 3 experimental groups were exposed to cigarette smoke for 20 weeks for 2 hours per day. After histopathological verification of smoke induced lung injury, montelukast (0.1 mg/kg) dissolved in Na(2)CO(3) was given in one group (MON), Na(2)CO(3) only was given in another group (MON control) and placebo was injected in the third group (COPD control) intraperitoneally for 21 days. At the end of this period blood samples were obtained for serum TNF-alpha assessment and light and electron microscopy analyses were performed on the lung tissues of sacrificed rats. Results: Serum TNF-a levels in the MON group were significantly lower than in the MON control and COPD control groups (38.84 +/- 4.9 pg/ml, 77.5 +/- 5.8 pg/ml and 79.2 +/- 6.9 pg/ml respectively, p < 0.05). Furthermore there was no statistically significant difference between the MON group and healthy controls with respect to serum TNF-alpha levels (38.84 +/- 4.9 pg/ml vs. 29.5 +/- 3.6 pg/ml, p > 0.05). Light and electron microscopic evaluation of the lungs demonstrated that the total histopathological damage score of the lung samples was significantly lower in the MON group than in MON controls and COPD controls (5.14 +/- 0.5, 8.4 +/- 0.6 and 8.7 +/- 0.4 respectively, p < 0.05), while there was no significant difference between the MON group and healthy controls (5.1 +/- 0.6 vs 2.3 +/- 0.2, p > 0.05). Conclusion: These findings suggest that montelukast might have a protective effect on smoke-induced lung injury in rats both from a histopathological and inflammatory point of view.Publication Open Access Antioxidant and anti-inflammatory effects of curcumin against hepatorenal oxidative injury in an experimental sepsis model in rats(TURKISH ASSOC TRAUMA EMERGENCY SURGERY, 2013) DULUNDU, ENDER; Savcun, Gulay Yilmaz; Ozkan, Erkan; Dulundu, Ender; Topaloglu, Umit; Sehirli, Ahmet Ozer; Tok, Olgu Enis; Ercan, Feriha; Sener, GokselBACKGROUND: To investigate the effects of curcumin, an antioxidant and anti-inflammatory agent, on free oxygen radicals and lipid peroxidation in an experimental sepsis model, as well as to determine the role of curcumin in preventing hepatorenal tissue damage caused by sepsis. METHODS: The rats were randomly divided into three groups (n=8) as follows: control group (group 1); sepsis group (group 2); and sepsis + curcumin group (group 3). Sepsis was created using the cecal ligation and perforation (CLP) method. Curcumin was administered intraperitoneally (200 mg/kg) in two equal doses just after the perforation and at twelve hours post-perforation. RESULTS: Serum TNF-alpha and IL-1 beta, and tissue MDA and MPO values were higher, whereas tissue GSH and Na+/K+-ATPase values were lower, in group 2 as compared to group 1. These values in group 3 were the inverse of those in group 2. As compared to group 1, histopathological evaluation of group 2 showed damaged hepatocytes, glomeruli, and tubules, whereas the damage was significantly reduced in group 3 as compared to group 2. CONCLUSION: The strong antioxidant and anti-inflammatory effects of curcumin against potential hepatorenal damage were shown using an experimental sepsis model in rats.Publication Metadata only Apocynin alleviates cisplatin-induced testicular cytotoxicity by regulating oxidative stress and apoptosis in rats(WILEY, 2019) ERCAN, FERİHA; Koroglu, Kutay M.; Cevik, Ozge; Sener, Goksel; Ercan, FerihaThe aim of this study was to investigate possible protective effects of apocynin (APO), an NADPH oxidase (NOX2) inhibitor, on cisplatin (CIS)-induced testicular damage. Four groups of Sprague Dawley rats were used: control, APO, CIS and CIS+APO. Following a single intraperitoneal dose of CIS (7 mg/kg), either dimethyl sulfoxide or APO (25 mg/kg) was administered orally for 5 days. Testis samples were evaluated microscopically for general histopathology and ultrastructure, proliferating and apoptotic cells, and NOX2 localization. Sperm parameters were evaluated. Malondialdehyde (MDA) and glutathione (GSH) levels and superoxide dismutase (SOD), myeloperoxidase (MPO) and 8-hydroxy-2-deoxyguanosine (8-OHdG) activities were analysed biochemically. The CIS group had a greater number of abnormal spermatozoa, atrophic seminiferous tubules, apoptotic and NOX2-immunoreactive cells; numerous large vacuole formations in the cytoplasm of germinal epithelial cells; degenerated intercellular tight junctions; higher MDA, 8-OHdG and MPO levels; decreased numbers of spermatozoa; and lower proliferative index and GSH and SOD levels. All these histologic and biochemical results were better in the CIS+APO group. CIS causes testicular damage by decreasing spermatogenic cell lines and increasing NOX2 activity and apoptosis through oxidative stress. APO prevents testicular damage, possibly by its antioxidant effects.Publication Open Access Apocynin ameliorates testicular toxicity in high-fat diet-fed rats by regulating oxidative stress(2023-03-01) ERTAŞ, BÜŞRA; ERCAN, FERİHA; Hersek İ., Coşkunlu B., Köroğlu M., Ertaş B., Şener G., Ercan F.Objective: The purpose of this study was to examine the effects of apocynin (APC), an inhibitor of NADPH oxidase (NOX), on high-fat diet (HF)-induced testis cytotoxicity.Methods: Wistar albino rats were divided into three groups as control, HF and HF+APC groups. Rats in HF and HF+APC groups were fed using HFfor 16 weeks and in the last four weeks of this period vehicle solution or APC (25 mg/kg) was administered orally five days a week, respectively.Control group was fed with standart lab chow for 16 weeks. Cholesterol, triglyceride, high-density lipoproteins, leptin, estrogen, testosterone,LH and FSH were estimated in blood serum. Sperm parameters were analysed from the epididymis. Testicular malondialdehyde, 8‐hydroxy‐2‐deoxyguanosine, glutathione, superoxide dismutase and myeloperoxidase levels were estimated biochemically. Testicular morphology,proliferative, apoptotic and NOX2-positive cells were analysed histologically.Results: HF-induced obesity caused significant alterations in serum lipid and hormone profiles. Testicular malondialdehyde, 8‐hydroxy‐2‐deoxyguanosine, and myeloperoxidase levels increased, glutathione and superoxide dismutase levels decreased in this group. Moreover,altered sperm parameters, increased degenerated seminiferous tubules, apoptotic and NOX2 – positive cells and decreased proliferative cellswere observed in the HF group. All these biochemical and histological alterations improved in the HF+APC group.Conclusion: HF-induced obesity causes altreations in lipid values, sperm parameters and testicular morphology by increasing oxidative stressthrough NOX2 activity. Apocynin might prevent testis damage via regulating oxidant/antioxidant balance.Publication Metadata only Apocynin attenuates testicular ischemia-reperfusion injury in rats(W B SAUNDERS CO-ELSEVIER INC, 2015) ŞİMŞEK, FERRUH; Sener, T. Emre; Yuksel, Meral; Ozyilmaz-Yay, Nagehan; Ercan, Feriha; Akbal, Cem; Simsek, Ferruh; Sener, GokselObjective: This study was designed to examine the possible protective effect of apocynin, a NADPH oxidase inhibitor, against torsion/detorsion (T/D) induced ischemia/reperfusion (I/R) injury in testis. Methods: Male Wistar albino rats were divided into sham-operated control, and either vehicle, apocynin 20 mg/kg-or apocynin 50 mg/kg-treated T/D groups. In order to induce I/R injury, left testis was rotated 720 degrees clockwise for 4 hours (torsion) and then allowed reperfusion (detorsion) for 4 hours. Left orchiectomy was done for the measurement of tissue malondialdehyde (MDA), glutathione (GSH) levels, myeloperoxidase (MPO) activity, and luminol, lucigenin, nitric oxide (NO) and peroxynitrite chemiluminescences (CL). Testicular morphology was examined by light microscopy. Results: I/R caused significant increases in tissue luminol, lucigenin, nitric oxide and peroxynitrite CL demonstrating increased reactive oxygen and nitrogen metabolites. As a result of increased oxidative stress tissue MPO activity, MDA levels were increased and antioxidant GSH was decreased. On the other hand, apocynin treatment reversed all these biochemical indices, as well as histopathological alterations that were induced by I/R. According to data, although lower dose of apocynin tended to reverse the biochemical parameters, high dose of apocynin provides better protection since values were closer to the control levels. Conclusion: Findings of the present study suggest that NADPH oxidase inhibitor apocynin by inhibiting free radical generation and increasing antioxidant defense exerts protective effects on testicular tissues against I/R. The protection with apocynin was more pronounced with high dose. (C) 2015 Elsevier Inc. All rights reserved.Publication Open Access Apocynin exhibits an ameliorative effect on endothelial dysfunction/atherosclerosis-related factors in high-fat diet-induced obesity in rats(2024-05-31) ERTAŞ, BÜŞRA; KOÇYİĞİT SEVİNÇ, SEVGİ; ERCAN, FERİHA; ORUN, OYA; AKKİPRİK, MUSTAFA; ÇETİNEL, ŞULE; Bulbul Ayci N., ERTAŞ B., Keles Kaya R., Kocyigit Sevinc S., Amuran G. G., ERCAN F., Sener G., ORUN O., AKKİPRİK M., ÇETİNEL Ş.Objective: The aim of this study was to reveal the effect of apocynin (APO) on the factors involved in obesity-related endothelial dysfunction (ED) and atherosclerosis (AS). Materials and Methods: Male Wistar albino rats were divided into control (CNT), high-fat diet (HFD) and HFD+APO groups. HFD and HFD+APO groups were fed HFD for sixteen weeks. APO (25 mg/kg) was administered to the HFD+APO group for the last four weeks. The effects of APO on: AS-related metabolic parameters (triglyceride, total cholesterol, high-density lipoprotein-cholesterol, insulin and leptin), oxidative stress (OS), [ malondialdehyde, glutathione, nicotinamide adenine dinucleotide phosphate (NADPH)-oxidase-2, oxidised-low-density lipoprotein (ox-LDL) and 8-hydroxy-2-deoxyguanosine], low-density lipoprotein and ox-LDL uptake potential (activin receptor-like kinase-1 and lectin-like oxidized low-density lipoprotein receptor-1, respectively), tissue inflammation (myeloperoxidase, monocyte-chemoattractant-protein-1, tumor necrosis factor-alpha), ED (endothelial-nitric oxide synthase, inducible-nitric oxide synthase, nitric oxide), programmed cell death (terminal deoxynucleotidyl-transferase-dUTP-nick-end labeling, cleaved-poly-ADP-ribose-polymerase, gasdermin-D N-terminal fragment, caspase-1), smooth muscle cell transformation (alpha-smooth muscle actin), histology and ultrastructure of thoracic aorta were evaluated. Results: In obesity, APO had an ameliorative effect on metabolic parameters, OS, inflammation, ED, programmed cell death and oxLDL uptake potential, but not on foam cell formation and LDL uptake potential. Conclusion: Apocynin may improve ED and AS in obesity by suppressing OS-linked factors involved in the early stage of AS.Publication Metadata only Aqueous garlic extract alleviates ischaemia-reperfusion-induced oxidative hepatic injury in rats(ROYAL PHARMACEUTICAL SOC GREAT BRITAIN, 2005) YEGEN, BERRAK; Sener, G; Sehirli, O; Ipci, Y; Ercan, F; Sirvanci, S; Gedik, N; Yegen, BCThis study was designed to examine the effects of aqueous garlic extract (AGE) on hepatic ischaemia-reperfusion (I/R) injury in rats. For this purpose, Wistar albino rats were subjected to 45min of hepatic ischaemia, followed by a 60-min reperfusion period. AGE (1 mL kg(-1), i.p., corresponding to 500 mg kg(-1)) or saline was administered twice, 15 min before ischaemia and immediately before the reperfusion period. Serum aspartate aminotransferase (AST) and alanine aminotransferase.(ALT) levels were determined to assess liver functions. Liver tissues were taken for the determination of malondialdehyde (MDA) levels, an end product of lipid peroxidation; glutahione (GSP.) levels, a key antioxidant; and myeloperoxidase (MPO) activity, as an indirect index of neutrophil inifiltration Hepatic collagen content, as a fibrosis marker, was also determined. Plasma ALT and AST activities, were elevated in the I/R group as compared with the control group, while these increases significantly decreased by AGE treatment. Hepatic GSH levels, significantly, depressed by I/R were elevated back to control levels in the AGE-treated I/R group. Increases in tissue MDA levels and MPO activity due to I/R injury were reduced back to control levels by AGE treatment. Similarly,, increased hepatic collagen content in the I/R group was reduced to the control level with AGE treatment. Since AGE administration alleviated the I/R-incluced injury of the liver and improved the hepatic and function, it seems likely that AGE, with its antioxidant and oxidant-scavenging properties, may be of potential therapeutic value in protecting the liver against oxidative injury due to ischaemia-reperfusion.Publication Metadata only Aqueous garlic extract inhibits protamine sulfate-induced bladder damage(KARGER, 2006) ERCAN, FERİHA; Zeybek, A; Cikler, E; Saglam, B; Ercan, F; Cetinel, S; Sener, GThis morphological and biochemical study aims to investigate the antioxidant effects of chronic administration of aqueous garlic extract (AGE) on protamine sulfate (PS)-induced bladder injury. Wistar albino female rats were catheterized and intravesically infused with phosphate-buffered solution (control group) or PS (PS group) dissolved in phosphate-buffered solution. In the PS + AGE group after the PS instillation, AGE (1 ml/kg, i. p., corresponding to 250 mg/kg) was injected intraperitoneally for 3 days. Bladder morphology was investigated by light and scanning electron microscopy. Tissue samples were also obtained to determine bladder malondialdehyde (MDA) and glutathione levels. In the PS group, ulcerated areas, an irregular mucus layer, inflammatory cell infiltration and an increased number of mast cells were observed. In the PS + AGE group a relatively normal urothelial topography, glycosaminoglycan layer and a decreased number of mucosal mast cells and inflammatory cells were observed. Increased MDA levels as a result of PS induction led us to propose that free radicals may have a critical role in this injury. The significant decrease in MDA and increase in glutathione levels in the PS + AGE group was in accordance with morphological findings. Based on the results, AGE treatment significantly prevented PS-induced degenerative morphological and biochemical changes of urinary bladder mucosa. Copyright (c) 2006 S. Karger AG, Basel.