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EMRE, GİZEM

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    In vitro and in silico evaluation of Centaurea saligna (K.Koch) Wagenitz-An endemic folk medicinal plant
    (ELSEVIER SCI LTD, 2018) EMRE, GİZEM; Zengin, Gokhan; Bulut, Gizem; Mollica, Adriano; Picot-Allain, Carene Marie Nancy; Mahomoodally, Mohamad Fawzi
    Centaurea saligna (K.Koch) Wagenitz is an endemic plant used in Turkish folk medicine as antibacterial, tonic, astringent, choleretic, anti-rheumatic, diuretic, and antipyretic. This study attempts for the first time to assess the possible enzyme inhibitory potential, antioxidant activity, and determine the phytochemical profile of the ethyl acetate, methanol, and water extracts of C. saligna. The water extract had the highest phenolic content (30.18 mg GAE/g extract) and the most potent oxidant scavenging activity ((120.53, 111.90, 68.43, and 157.88 mg TEN extract, for CUPRAC [cupric reducing antioxidant capacity], FRAP [ferric reducing antioxidant power], DPPH [2,2-dipheny1-1-picrylhydrazyl], and ABTS [2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid] assays respectively)). The water extract (4.16 mg KAE/g extract) also inhibited tyrosinase and contained high level of catechin (214 mu g/g extract). Ethyl acetate extract showed potent inhibitory capacity against cholinesterases (2.22 and 2.21 mg GALAE/g extract for acetyl and butyryl cholinesterase, respectively) and alpha-glucosidase (23.80 mmol ACAE/g extract). High concentration of apigenin (2472 mu/g extract) was identified in the ethyl acetate extract. In silico studies showed that apigenin binds to the enzymatic pocket of alpha-glucosidase and is stabilised by a network of hydrogen bonds and pi-pi stacking. Data collected in the present study advocates the need for further investigation geared towards validation of C. saligna for the management of complications related to the target enzymes, such as diabetes type II, Alzheimer's disease, and epidermal hyperpigmentation. (C) 2018 Elsevier Ltd. All rights reserved.
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    HPLC-MS/MS-based metabolic profiling and pharmacological properties of extracts and infusion obtained from Amelanchier parviflora var. dentata
    (ELSEVIER SCIENCE BV, 2018) DOĞAN, AHMET; Zengin, Gokhan; Diuzheva, Alina; Jeko, Jozsef; Cziaky, Zoltan; Bulut, Gizem; Dogan, Ahmet; Haznedaroglu, Mehmet Zeki; Rengasamy, Kannan R. R.; Lobine, Devina; Bahadori, Mir Babak; Mahomoodally, Mohamad Fawzi
    Arnelanchierparviflora var. dentata is an endemic medicinal plant traditionally consumed to treat stomach pain, cardiovascular diseases, and urinary tract infections in Turkey's folk medicine. Although Amelanchier genus is an excellent source of phytochemicals with a panoply of biological activities, no report has been made available on Amelanchier parviflora var. dentata. Thus, the metabolic profiles of methanol, ethyl acetate, and water extracts were obtained using HPLC-MS/MS. Besides, the pharmacological properties such as antioxidant activity and enzyme inhibition effects against acetyl/butyrylcholinesterase, tyrosinase, alpha-amylase, and alpha-glucosidase were evaluated. In total, 62, 38, and 16 metabolites were Identified from the methanolic, water, and ethyl acetate extracts, respectively. The identified metabolites belong to anthocyanins, phenolic acids, and flavonoids. The tested extracts exhibited different enzyme inhibitory activity against cholinesterases (acetylcholinesterase: 0.52-2.47 mg GEs/g extract and butyrylcholinesterase: 0.47-3.59 mg GEs/g extract), tyrosinase (19,0-145.54 mg KAEs/g extract), alpha-amylase (0.23-1.10 mmol ACEs/g extract), and alpha-glucosidase (8.89-9.92 mmol ACEs/ g extract). Evidence from the present study supports the necessity for more research in an endeavor to discover biologically active secondary metabolites from A. parviflora to manage specific ailments.
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    New insights into the in vitro biological effects, in silico docking and chemical profile of clary sage - Salvia sclarea L.
    (ELSEVIER SCI LTD, 2018) DOĞAN, AHMET; Zengin, Gokhan; Senkardes, Ismail; Mollica, Adriano; Picot-Allain, Carene Marie Nancy; Bulut, Gizem; Dogan, Ahmet; Mahomoodally, M. Fawzi
    Salvia sclarea L. is traditionally used to manage common human ailments and is consumed as a food product. This study aimed to establish the phytochemical profile and antioxidant potential of ethyl acetate, methanol, and water extracts of Salvia sclarea. The inhibitory action of the extracts against alpha-amylase, alpha-glucosidase, acetyicholinesterase, butyrylcholinesterase, and tyrosinase was also investigated. Methanol extract showed the highest phenolic and fiavonoid contents (81.78 mg GAE/g extract and 40.59 mg RE/g extract, respectively). Reversed phase high performance liquid chromatography with diode array detector analysis revealed that S. sclarea was rich in rosmarinic acid. The water extract exhibited the lowest inhibitory activity against alpha-amylase but the upmost activity against alpha-glucosidase (0.19 and 18.24 mmol ACAE/g extract, respectively). Experimental data showed that only the water extract (8.86 mg KAE/g extract) significantly inhibited tyrosinase. Docking studies showed that quercetin binds to tyrosinase by two hydrogen and a pi-pi bonds. Salvia sclarea showed interesting biological activity against key enzymes involved in the pathogenesis of common ailments. (C) 2018 Elsevier Ltd. All rights reserved.