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CÜCÜ, AYŞEN

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    PublicationOpen Access
    A systematic study of determination and validation of finasteride impurities using liquid chromatography
    (ELSEVIER, 2020-02) CÜCÜ, AYŞEN; Cucu, Aysen Kurt; Sakarya, Serap; Demir, Hulya Demircan; Ahmad, Sarfraz
    Pharmaceutical use of finasteride (Dilaprost (R)) has been well documents in the peer-reviewed literature; however, the presence of trace amounts of related substances (impurities) in finasteride may influence the tharapeutic efficacy and safely. Due to limited information available, the objective of this study was to develop a quantification method for the three impurities of finasteride using high performance liquid chromatography (HPLC) with an ultraviolet (UV) detector. The compounds (impurities) of finasteride that are registered with the European Pharmacopeia, which we sought to validate are: -N-(1,1-dimethylethyl)-3-oxo-4-aza-5 alpha-androstane-17 beta-carboxa mide (impurity A), methyl 3-oxo-4-aza-5 alpha-androst-1-ene-17 beta-carboxylate (impurity B), and -N-(1,1-dimehylethyl)-3-oxo-4-azaandrosta-1,5-diene-17 beta-carboxamide (impurity C). Analyses were performed using a Nova Pac C-18 column for HPLC with isocratic elution. Detection was carried out at 210 nm, the concentration of the three impurities was in the range was 1.5-4.5 mu g mL(-1) at ambient temperature with a mobile phase of water + acetonitrile + tetrahydrofuran (80:10:10, v/v/v) and the flow rate was 2.0 mL min(-1). The recoveries were: 101.35 +/- 0.62% (impurity A), 101.60 +/- 2.66% (impurity B) and 101.97 +/- 2.05% (impurity C). Validation of the method yielded fairly good results as it relates to the precision and accuracy. It is, therefore, concluded that the method would be suitable for not only the separation and determination of processed impurities to monitor the reactions, but also for the quality assurance of finasteride and its related substances. (C) 2019 Production and hosting by Elsevier B.V. on behalf of King Saud University.
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    PublicationOpen Access
    Determination and stability of some international screening limited drugs in equine blood by LC-MS/MS
    (ANKARA UNIV PRESS, 2019-10-01) CÜCÜ, AYŞEN; Goktas, Eylem Funda; Kabil, Erol; Gunes, Ertugrul; Korkmaz, Serol; Kadioglu, Tuba Asena; Cucu, Aysen Kurt
    Stability of some international screening limited therapeutic substances for doping control in equine blood has been investigated with a validated method by using LC-MS/MS after chemical hydrolysis with orthophosphoric acid. Whole blood samples were extracted with a WAX cartridge on auto-SPE. Stability test of the drug substances performed at the same time in working solution and matrix at +4 degrees C, -20 degrees C, +20 degrees C in dark and +20 degrees C in light conditions for 1, 2, 3 and 4 weeks. For short-time stability, the effects of 6 h storage at +55 degrees C was also investigated. Repeated data were statistically analyzed with ANOVA. All substances in working solution were stable at -20 degrees C and +4 degrees C. In the blood matrix, higher temperatures (+20 degrees C) in light and dark caused degradation of substances at the end of four weeks and short-term study (P<0.05). In conclusion, these international screening limited substances in blood matrix could be unstable related to temperature and storage time, although in working solution they could be mostly stable in various temperatures for four weeks.
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    PublicationOpen Access
    Quantitative Determination of The Heavy Metals (Lead, Zinc And Manganese) in White Cheese Produced in Ergene Basin by Using Atomic Absorption Spectrophotometry
    (MARMARA UNIV, FAC PHARMACY, 2015-04-09) CÜCÜ, AYŞEN; Cucu, Aysen Kurt; Yavuz, Mustafa; Demir, Hulya Demircan
    It is well known that heavy metals are harmful for human health appearing only after exceeding the specific concentrations of each metal. This study was carried out to determine the concentrations of heavy metals in cheese that was produced in diaries of Ergene Basin. The dairies near the Ergene River were investigated and suitable ones were selected. Since the sampling process directly affects the sensitivity of the results, particular attention was given to this process. A total of twelve samples of cheese were collected from different diaries and each samples were digested three times by dry ashing and wet ashing methods and totally 108 cheese samples were obtained for metal analyses. The lead, manganese content of the cheese samples were measured by graphite furnace atomic absorption spectrophotometer and zinc content of the cheese samples were measured by flame atomic absorption spectrophotometer. Contents of heavy metals in the examined samples were compared with the excepted limit values in the literature and the results were evaluated. Experimental data shows that the concentrations of heavy metals are in the range of accepted limit values.
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    PublicationOpen Access