Person: AK, ESİN
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AK
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ESİN
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Publication Open Access Comparison of the protective effect of alpha lipoic acid and quercetin in methotrexate- induced lung damage(2023-03-01) AK, ESİN; OKTAY, NİHAL ŞEHKAR; AK E., MUHAN A., ÇALIŞKAN Ş., OKTAY N. Ş.Objective: The aim of this experiment is to investigate and compare the effects of alpha lipoic acid (ALA) and quercetin (QUE) on methotrexate (MTX)-induced lung injury in rats. Method: Wistar Albino rats were distributed into control, MTX, MTX+ALA and MTX+QUE groups with each consisting of 6 rats. Except control group, MTX administrated to rats as a single dose (20 mg/kg) intraperitoneally (i.p.) on the first day. Saline (0.1 cc/100 gr/day, i.p.) was injected to rats in control and MTX groups for 5 days. In MTX+ALA and MTX+QUE groups, rats had injections of ALA (50 mg/kg/day, i.p.) and QUE (50 mg/kg/day, i.p.) for 5 days. After sacrification on day 6, lung tissues were excised out for histopathologic and biochemical investigation. Results: MTX group showed massive hemorrhage with edema in the interstitium, significant inflammatory cell infiltration, and severe alveolar destruction and vascular congestion. Additionally, significant increases in oxidative stress markers as malondialdehyde and sialic acid and significant decreases in antioxidants as glutathione, superoxide dismutase and catalase were detected at the tissue level in MTX group (p<0.0001, p<0.0001, p<0.0001, p=0.03 and p<0.0001, respectively). Both ALA and QUE treatment led to a prominent improvement in morphologic damage. Moreover, ALA and QUE resulted in the reversal of the alterations seen in the tissue oxidative damage markers and antioxidant activities as well. We could not reveal a significant difference between MTX+ALA and MTX+QUE group in terms of morphologic damage and biochemical markers of oxidative injury (p>0.05). Conclusion: Our study showed the similar protective effect of ALA and QUE in MTX induced lung damage. Further studies are warranted to verify the results of our outcome.Publication Open Access Tooth replantation with adipose tissue stem cells and fibrin sealant: microscopic analysis of rat's teeth(SPRINGER INTERNATIONAL PUBLISHING AG, 2016-12) YARAT, AYŞEN; Demirel, Sezin; Yalvac, Mehmet Emir; Tapsin, Sidika; Akyuz, Serap; Ak, Esin; Cetinel, Sule; Yarat, Aysen; Sahin, FikrettinTreatment for dental avulsion cases is early or late replantation of the traumatized teeth. Prognosis of the replanted tooth depends on the level of periodontal injury. Adipose tissue stem cells (ATSCs) were reported to improve periodontal ligament tissue (PDL) regeneration. Fibrin sealant (FS) contains thrombin and fibrinogen to form an adhesive fibrin clot routinely used in surgical procedures. Here, we aimed to investigate the effects of ATSCs + FS treatment on healing of PDL after tooth replantation in a rat model. After 60 min of extraction, maxillary central incisor teeth were replanted with ATSCs + FS. Two months later, the rats were sacrificed and hemimaxilla blocks were dissected out for histological analysis. The results showed that there was a significant improvement in histological findings of ATSCs + FS treated group compared to only FS treated and non-treated groups corresponding to reduced inflammatory resorption and increased new PDL formation. Furthermore, the ankylosis levels were lowered after ATSCs + FS treatment. Singular use of FS improved PDL healing moderately. Our results indicated that ATSCs + FS treatment improves PDL healing after tooth replantation suggesting a new therapeutic potential in the treatment of dental avulsion cases.Publication Open Access Investigation of the Effects of Edaravone on Valproic Acid Induced Tissue Damage in Pancreas(MARMARA UNIV, FAC PHARMACY, 2017-06-20) YARAT, AYŞEN; Oktay, Sehkar; Alev-Tuzuner, Burcin; Tunali, Sevim; Ak, Esin; Emekli-Alturfan, Ebru; Tunali-Akbay, Tugba; Koc-Ozturk, Leyla; Cetinel, Sule; Yanardag, Refiye; Yarat, AysenValproic acid (VPA), an effective antiepileptic and anticonvulsant drug, has some toxic side effects due to causing elevated oxidant production. The aim of this study is to investigate the effects of edaravone, a potent free radical scavenger on VPA induced toxicity and tissue damage by biochemical and histological examinations on pancreas. Female Sprague Dawley rats were divided into four groups as follows; control, edaravone, VPA, VPA+edaravon. VPA and edaravone were injected intraperitonally for seven days. Total protein, lipid peroxidation (LPO), sialic acid (SA) and glutathione (GSH) levels and alkaline phosphatase (ALP), tissue factor (TF), superoxide dismutase (SOD), glutathione-S-transferase GST), catalase (CAT), glutathione peroxidase (GPx) and myeloperoxidase (MPO) activities were determined in pancreas homogenates. In VPA given group, LPO and SA levels, and ALP, TF, MPO activities significantly increased and GST, CAT, GPx activities significantly decreased compared to control group. A marked morphological damage was detected in the VPA group. Ameliorative effects of edaravone were observed in SA, TF, CAT, GPx parameters and histological examination in the VPA group. Therefore, edaravone may be effective in moderation and/or reduction of toxic effects of VPA on pancreas.Publication Open Access White LED Light Exposure Inhibits the Development and Xanthophore Pigmentation of Zebrafish Embryo(NATURE RESEARCH, 2019-12) ALTURFAN, EBRU IŞIK; Ustundag, Unsal Veli; Caliskan-Ak, E.; Ates, Perihan Seda; Unal, Ismail; Egilmezer, Gizem; Yigitbasi, Turkan; Alturfan, A. Ata; Emekli-Alturfan, EbruCircadian rhythm in all living organisms is disturbed continuously by artificial light sources and artificial lighting has become a hazard for public health. Circadian rhythm of melatonin maintains high levels of melatonin during the night and low levels during the day. N-acetyltransferase (arylalkylamine N-acetyltransferase, AANAT) is one of the four enzymes required for melatonin synthesis and mtnr1ba is a melatonin receptor-encoding mRNA that is expressed widely in the embryonic brain. Pax7 has important roles during neural crest development and especially xanthophore pigmentation. Due to its diurnal nature, zebrafish provide a special opportunity for research on circadian rhythms that are regulated by melatonin. Here in this study, we showed that when compared with the white light control group, white LED light exposure resulted in loss of yellow pigmentation, decreased body length and locomotor activity, oxidant-antioxidant imbalance and decreased expressions of aanat2, mtnr1ba, and pax7 in zebrafish embryos. Histological analysis of this group revealed disorganization of the spaces among photoreceptor cells, decreased total retinal thickness and photoreceptor cell layer thickness compared with the control group. Artificial lighting pollution has the potential to become an important risk factor for different diseases including cancer especially for industrialized countries, therefore, more studies should be performed and necessary regulations should be made regarding this risk factor.Publication Open Access Publication Open Access Determination of Storage Time of Saliva Samples Obtained From Patients With and Without Chronic Periodontitis for the Comparison of Some Biochemical and Cytological Parameters(WILEY, 2013-07) YARAT, AYŞEN; Emekli-Alturfan, Ebru; Yarat, Aysen; Caliskan-Ak, Esin; Pisiriciler, Rabia; Kuru, Bahar; Noyan, UlkueBackground: Salivary glutathione (GSH), malondialdehyde (MDA), protein, sialic acid (SA) levels, cytological parameters, and tissue factor activities (TFa) were investigated when fresh and after 3, 7, 11, 15, 21, and 30 days (d) of storage at -20 degrees C both in the control and the periodontitis group. Moreover, the control and the periodontits groups were compared and continuity of the significances detected between the two groups were evaluated. Methods: GSH, MDA, SA, protein, and TFa were determined using the methods of Beutler, Yagi, Warren, Lowry, and Quick, respectively. Saliva imprint samples were stained with Giemsa and microscopically examined. Results: When the continuity of the significances of differences between the two groups was investigated, differences continued to be significant for GSH and TFa on days 3, 7, 11, 15, 21, and 30. Cytologically, only the significance detected between leucocyte numbers continued to be significant for 30d. However significance of differences in total protein, MDA, and SA levels on day 0, were interrupted on days 3, 7, and 11, respectively. Conclusion: Saliva samples may be stored for 30d for GSH and TFa analyses in patients with and without periodontitis. However, to compare salivary MDA, SA, and total protein levels in these groups we suggest fresh samples to be studied. (C) 2013 Wiley Periodicals, Inc.Publication Open Access Protective role of progesterone on lung injury induced by ischemia reperfusion of the lower limbs(2022-01-01) AK, ESİN; OKTAY, NİHAL ŞEHKAR; ÖZBEYLİ, DİLEK; AK, KORAY; AK E., OKTAY N. Ş., ÖZBEYLİ D., Muhan A., AK K.Objective: Remote lung injury is one of the most challenging issues in patients undergoing ischemia reperfusion (IR) injury of the lower limbs. We examined the role of progesterone (PG) on the remote injury of the lungs seen after IR of the lower limbs. Methods: Eighteen male Sprague Dawley rats were divided into three groups. (1) Control: rats had only two physiological saline injections intraperitoneally (i.p.) 2 h apart under general anesthesia. (2) IR: underwent 2 h tourniquet induced ischemia for both lower limbs followed by 2 h of reperfusion. Animals were injected with physiological saline (i.p.) before both ischemia and reperfusion. (3) IR + PG: rats underwent the same IR protocol as the IR group and were injected with PG (16 mg/kg, i.p.) before both ischemia and reperfusion. After reperfusion, rats were sacrificed, and lung tissues were taken out for histopathologic and biochemical analyses. Results: In IR + PG group, tissue levels of malondialdehyde and nitric oxide decreased significantly compared with the IR group (p<0.01). Similarly, glutathione level and superoxide dismutase and glutathione S-transferase activities significantly increased in the IR + PG group than in the IR group (p<0.05, p<0.01, and p<0.05, respectively). In light microscopy, reduced inflammatory cell infiltration, amelioration in alveolar structure, and mild vascular congestion in the parenchyma were seen in the IR + PG group. A significant improvement in histopathologic score was seen in the IR + PG group compared with the IR group (p<0.001). Conclusion: PG might be effective in attenuating remote injury of the lung in lower body IR via its antioxidant functionPublication Open Access A Histological Evaluation of the Effect of Ghrelin on Wound Healing in Rats(2022-12-01) AK, ESİN; ERCAN, FERİHA; AK E., ULUSOY-DAĞ K., ERCAN F., ÇORAK A.Objective: This study aimed to investigate the potential effects of ghrelin on wound healing. Materials and Methods: Sprague-Dawley rats were divided into 3 groups: Control (n = 8), wound-saline (W+S, n = 16), and wound-ghrelin (W+Gr, n = 16). A wound was created on the cervical back region of rats using an 8 mm biopsy punch tool in the W+S and W+Gr groups. Either saline (1 mL/kg) or ghrelin (10 ng/kg) was administrated intraperitoneally each day to the rats in the non-control groups after the onset of the wound. Rats from the W+S and W+Gr groups were euthanized on the 7th (n = 8 from each group) and 14th day (n = 8 from each group) of the experiment. The histopathological score was evaluated statistically using one-way analysis of variance and Tukey’s multiple comparison tests. Results: The rats euthanized from the W+S group on day 7 (subgroup W+S7) showed degenerated epidermis, no hair follicles, presence of granulation tissue, inflammatory cell infiltration, vasocongestion, and increased collagen fibers in dermis. However, all these histopathological findings significantly decreased in the rats euthanized from the W+Gr group on day 7 (subgroup W+Gr7) compared to the W+S7 group (p < 0.05). The W+S14 group showed thick epidermis, a few hair follicles, angiogenesis, and increased collagen fibers in the dermis. Additionally, the histopathological findings decreased significantly in the W+Gr14 group compared to W+S14 group (p < 0.05). Conclusion: Based on the statistical analysis of the histological findings, the ghrelin treatment appears to have a beneficial effect on wound healing. Keywords: Wound healing, ghrelin and histologyPublication Open Access Protective role of white cabbage extract against amiodarone-induced lung damage in rats(2022-03-01) AK, ESİN; AK E., TÜRKYILMAZ İ. B., MUHAN A., YANARDAĞ R.Amaç: Bu çalışmada amiodaron’un sebep olduğu akciğer hasarında beyaz lahananın koruyucu etkileri araştırılmıştır. Materyal ve Metot: Bu çalışmada, sıçanlar 4 gruba ayrılmıştır. Kontrol grup: dokunulmamış sıçanlar, WCAE grup, sıçanlara 7 gün 500 mg/kg beyaz lahana ekstraktı verildi; AMD grup, sıçanlara 7 gün 100 mg/kg amiodaron (AMD) verildi; AMD+WCAE grup, sıçanlara aynı dozlarda beyaz lahana ekstraktı ve AMD verildi. Bulgular: AMD+WCAE grubunda, beyaz lahana ekstraktı tedavisi interstisyal ödem ve konjesyonun azalmasına, alveolar yapılarda iyileşme ve bunun yanı sıra parankimdeki enflamatuvar hücre infiltrasyonun gerilemesine sebep olmuştur. Ek olarak, AMD+WCAE grubunda parankimde kollajen liflerinin birikmesinde önemli derecede bir azalma görülmüştür. Akciğerdeki glutatyon seviyesi, total antioksidan kapasitesi ve glutatyon-S-transferaz, paraoksonaz and karbonik anhidraz aktiviteleri azalırken, lipit peroksidasyon, ileri okside protein ürünleri, total oksidan durumu, reaktif oksijen türleri, oksidatif stres indeksi, nitrik oksit ve hidroksiprolin seviyeleri, katalaz, süperoksit dismutaz, glutatyon peroksidaz, glutatyon redüktaz, laktat dehidrogenaz and ksantin oksidaz aktiviteleri AMD grubunda artmıştır. Beyaz lahana ekstraktı tedavisi AMD’un neden olduğu bu seviyeleri ve aktiviteleri tersine çevirmiştir. Sonuç: Beyaz lahana ekstraktının amiodaron’un sebep olduğu akciğer hasarını azaltabileceği sonucuna varabiliriz. Anahtar Kelimeler: Akciğer, amiodaron, beyaz lahana ekstraktı, oksidatif stresPublication Open Access Protective effect of baicalin on cyclophosphamide-induced oxidative stress and morphologic damage in testis tissue(2024-01-01) ALTAY, GÜLSEREN; OKTAY, NİHAL ŞEHKAR; AK, ESİN; ALTAY AZNAVUR G., OKTAY N. Ş., AK E.The purpose of this study is to examine the effect of baicalin on cyclophosphamide (CP)-induced testicular damage in rats. Twenty-eight Wistar albino rats were assigned to the control, baicalin, CP, and CP+Baicalin groups. A single dose of CP (200 mg/kg) was induced intraperitoneally (i.p.) in the CP and CP+Baicalin groups. Baicalin (100 mg/kg/day, i.p.) was administered in the baicalin and CP+ Baicalin groups for 6 days. After sacrification, the testes, epididymis and blood samples were taken. Testis tissues were investigated for general morphology, proliferating, and apoptotic cells. Oxidative stress parameters in testis tissue and hormone levels in serum were examined by biochemical analysis. Sperm analysis was performed on smear samples obtained from the epididymis. In CP + Baicalin group, decreases in both the number of abnormal spermatozoa and degeneration of seminiferous tubules were observed compared to the CP group. Also, decreased apoptotic cells and increased proliferative cells were detected in these seminiferous tubules. Furthermore, the treatment with Baicalin reversed the changes observed in the markers of oxidative damage including malondialdehyde, total oxidant status, and oxidative stress index, and markers of the antioxidant defense system including glutathione, total antioxidant capacity, superoxide dismutase, and catalase except for glutathione s-transferase (GST) in testicular tissue. Decreased serum levels of follicle-stimulating hormone, luteinizing hormone, and testosterone related to CP significantly improved in CP + Baicalin group. Conclusion: Baicalin attenuates CP-induced testicular damage by inhibiting oxidative stress possibly due to its antioxidant properties. Considering on the proposed beneficial effects of Baicalin against testicular damage induced by CP, it might have the potential to be a promising agent for male infertility related to chemotherapy.