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ŞİŞMAN, ALPER

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ŞİŞMAN

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ALPER

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Subject: Bioengineering ×

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    PublicationOpen Access
    Developing a surface acoustic wave-induced microfluidic cell lysis device for point-of-care DNA amplification
    (2023-01-01) ŞİŞMAN, ALPER; HUSSEINI A. A., Yazdani A. M., Ghadiri F., ŞİŞMAN A.
    We developed a microchip device using surface acoustic waves (SAW) and sharp-edge glass microparticles to rapidly lyse low-level cell samples. This microchip features a 13-finger pair interdigital transducer (IDT) with a 30-degree focused angle, creating high-intensity acoustic beams converging 6 mm away at a 16 MHz frequency. Cell lysis is achieved through centrifugal forces acting on Candida albicans cells and glass particles within the focal area. To optimize this SAW-induced streaming, we conducted 42 pilot experiments, varying electrical power, droplet volume, glass particle size, concentration, and lysis time, resulting in optimal conditions: an electrical signal of 2.5 W, a 20 μL sample volume, glass particle size below 10 μm, concentration of 0.2 μg, and a 5-min lysis period. We successfully amplified DNA target fragments directly from the lysate, demonstrating an efficient microchip-based cell lysis method. When combined with an isothermal amplification technique, this technology holds promise for rapid point-of-care (POC) applications.