Person: ŞENER, GÖKSEL
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ŞENER
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GÖKSEL
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Publication Metadata only Spectrochemical and biochemical assay comparison study of the healing effect of the Aloe vera and Hypericum perforatum loaded nanofiber dressings on diabetic wound(PERGAMON-ELSEVIER SCIENCE LTD, 2021) ÖZBEYLİ, DİLEK; Guleken, Zozan; Depciuch, Joanna; Ege, Hasan; Ilbay, Gul; Kalkandelen, Cevriye; Ozbeyli, Dilek; Bulut, Huri; Sener, Goksel; Tarhan, Nevzat; Kuruca, Serap ErdemDiabetic wounds have a slow healing process and easy to be infected. In addition to current drug treatments, supportive approaches are needed for diabetic wound treatment. In this study, we aimed to load Aloe Vera (AV) and Hypericum perforatum oil (HPO) with PCL/Ge (Poly (e-caprolactone)/Gelatine) polymeric biodegradable by electrospinning method into nanofiber dressings on an experimental diabetic wound model to compare the diabetic wound healing effect. Changes in the amount and chemical structure of phospholipids, proteins, and lipids were investigated in the blood and serum samples of the animals using Fourier transform infrared (FTIR) analysis. To evaluate biological events associated with the wound repair process in inflammatory phase we used oxidant and antioxidant status to determine the healing status of wounds such as Total antioxidant status (TAS), Total oxidant level (TOS) and tumor necrosis factor alpha (TNF-alpha) levels. TOS level increased in DM groups and decreased in the AV and HPO group. Oxidative stress index decreased and TNF-alpha level increased in the HPO group. FTIR spectra showed changes in the phospholipids, proteins, and carbon chain of lipids in the whole blood as well as serum of DM rats. FTIR spectra combined with Principal component analysis (PCA) showed, that treated DM rats by AV and HPO caused return chemical structure of blood and serum to this observed in control group. Higher similarity with control group for HPO rats was observed. HPO is better than AV in the alternative for healing on diabetic wound. Thus, we have demonstrated that IR spectroscopy and mul-tivariate data analysis and biochemical assays are consistent and correlative with each other. (C) 2021 Elsevier B.V. All rights reserved.Publication Metadata only Melatonin prevents deterioration of erectile function in streptozotocin-induced diabetic rats via sirtuin-1 expression(WILEY, 2020) ŞEKERCİ, ÇAĞRI AKIN; Sahan, Ahmet; Akbal, Cem; Tavukcu, Hasan Huseyin; Cevik, Ozge; Cetinel, Sule; Sekerci, Cagri Akin; Sener, Tarik Emre; Sener, Goksel; Tanidir, YilorenA review of the literature indicated that sirtuin-1 expression, a regulator of nitric oxide bioavailability in erectile dysfunction (ED) after melatonin therapy, has not yet been investigated. The objective of this study was to evaluate the protective effects of melatonin for erectile function with sirtuin-1 protein expression in type 1 diabetic rat models. Fifty male Sprague Dawley rats were placed into five groups. Except for those in the control group (C), each animal received a single dose (60 mg/kg) of streptozotocin to induce diabetes. The animals were placed into the diabetes (D) group, insulin (I) group (6 U/kg/day), melatonin (Mel) group (10 mg kg(-1) day(-1)) and combined treatment (I + Mel) group. Ten weeks later, the serum testosterone levels, intracavernosal pressure (ICP), mean arterial pressure (MAP), malondialdehyde (MDA), cyclic guanosine monophosphate (c-GMP), 8-hydroxydeoxyguanosine (8-OHdG), nitric oxide synthase (NOS), caspase-3 activity, sirtuin-1 and endothelial nitric oxide synthase (eNOS) protein expression and histological findings were assessed. The mean ICP/MAP ratio for the D group was lower than the mean ratios for the other groups. The treatment groups, particularly the I + Mel group, exhibited lower 8-OHdG and MDA levels and caspase-3 activity than the D group. The sirtuin-1 and eNOS expression and cavernosal tissue (CT) histology seemed to have been preserved by the melatonin and/or insulin therapy. These results were indicative of a profound protective effect of melatonin by the activation of sirtuin-1 protein expression against hyperglycemia-induced oxidative CT injury.Publication Open Access Effects of Exercise and Calorie Restriction on Brain_x000D_ and Testis in Natural Aging Model(2021-04-25) YARAT, AYŞEN; Umay HAKGÜDER;Hazal İPEKÇİ;Tuğba TUNALI AKBAY;Ayşen YARAT;Ebru Emekli ALTURFAN;Ünsal Veli ÜSTÜNDAĞ;Burçin ALEV;Nevin GENC KAHRAMAN;Reyhan ÖZÇELİK;Göksel ŞENERThe aim of our study was to examine the effects of exercise and calorie_x000D_ restriction on various tissue damage and antioxidant parameters_x000D_ in the brain and testis of rats in a natural aging model. For this_x000D_ purpose, male Sprague-Dawley rats were the control group (C),_x000D_ the elderly (A), the elderly with calorie restriction (ACR), the elderly_x000D_ who were exercised (AE) and the elderly who were exercised with_x000D_ calorie restriction (ACRE), they were divided into 5 groups. The control_x000D_ group was composed of three-month-old animals. The other_x000D_ group consisted of 15-month-old rats. Exercise and calorie restriction_x000D_ were applied for 6 weeks. At the end of the experiment, lipid_x000D_ peroxidation (LPO), nitric oxide (NO), glutathione (GSH) levels and_x000D_ superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase_x000D_ (GST) and tissue factor (TF) were determined in brain and_x000D_ testicular tissues homogenates. As a result of the study, the A_x000D_ group’s brain and testis LPO, NO levels and TF activity increased,_x000D_ GSH levels and SOD, CAT and GST activities decreased, when compared_x000D_ to the C group. As a result of our study, an increase in oxidant_x000D_ damage was observed with TF activity in the brain and testis in the_x000D_ natural aging model, and positive effects of exercise and calorie_x000D_ restriction on the antioxidant levels in the brain were determined,_x000D_ especially in aging.Publication Metadata only Mesenchymal stem cell therapy improves erectile dysfunction in experimental spinal cord injury(SPRINGERNATURE, 2020) ERTAŞ, BÜŞRA; Albayrak, Omercan; Sener, Tarik Emre; Ersahin, Mehmet; Ozbas-Turan, Suna; Ekentok, Ceyda; Tavukcu, Hasan Huseyin; Cevik, Ozge; Cetinel, Sule; Ertas, Blisra; Sener, GokselThe aim of this study is to investigate the therapeutic potential of adipose-derived mesenchymal stem cell (AD-MSC) from brown adipose tissue on erectile dysfunction (ED) in experimentally induced spinal cord injury in rats. 24 male Wistar rats were divided into 3 groups; control, spinal cord injury (SCI) + vehicle, and SCI + AD-MSC. To induce SCI, a standard weight-drop method that induced a moderate to severe injury (100 g/cm force) at T7-T10, was used. AD-MSC (3 x 105 cells /5 mu L) was applied by local transplantation into the region of injury. At the end of four-weeks, rats underwent neurological examinations and then intracavernosal and mean arterial pressures (ICP and MAP) measurements. After decapitation, spinal cord and cavernosal tissue samples were taken to analyze neuronal nitric oxide synthase (n-NOS), proto-oncogene protein c-FOS and nerve growth factor (NGF). Tissues were also examined histologically. Spinal cord injury caused decrease on NGF and n-NOS levels while c-FOS was increased. The ICP/MAP value in vehicle-treated SCI rats was found to be significantly higher than the control group. On the other hand, in SCI + AD-MSC group, all these parameters were reversed back to control levels. AD-MSC therapy may be beneficial against erectile dysfunction in experimentally induced SCI by ameliorating neuronal damage.Publication Open Access Protective effects of quercetin against cisplatin induced urogenital organ toxicity(MARMARA UNIV, 2020-09-11) ERCAN, FERİHA; Sener, Tarik Emre; Cadirci, Selin; Cevik, Ozge; Ercan, Feriha; Koroglu, M. Kutay; Sakarcan, Selin; Sener, GokselCisplatin is a chemotherapeutic agent that is the first to enter treatment from organic platinum-derived drugs. Nephrotoxicity and cytotoxicity are major factors that limit its use. The aim of the study is to investigate the possible protective effects of quercetin against cisplatin-induced urogenital organ toxicity. In our study, Sprague Dawley four month old male rats were divided into 4 groups; control + saline (SF), control + quercetin (20 mg/kg for 21 days), cisplatin (7 mg/kg as a single dose) + SF and cisplatin + quercetin groups. After decapitation, the kidney, bladder, testis and corpus cavernosum tissue samples were taken to analyze caspase-3, an index of apoptosis, and oxidative stress parameters such as malondialdehyde (MDA), gluta-thione (GSH), and 8-hydroxy-2'-deoxyguanosine (8-OHdG). Furthermore, tissues were also examined histologically. Cisplatin caused significant increases in MDA and 8-OHdG levels, demonstrating increases in lipid peroxidation and oxidative DNA damage, respectively, in all tissues. In parallel with the oxidant stress increase, the endogenous strong antioxidant GSH levels were decreased. Caspase activity and caspase 3 expressions, which we measured as an indicator of apoptosis, increased significantly with cisplatin treatment. On the other hand, treatment with quercetin, a powerful antioxidant flavonoid, reversed these changes. Histological findings also demonstrated well-preserved tissues due to quercetin treatment. In conclusion, our results suggested that quercetin, when given with cisplatin, can be protective against the chemotherapeutic induced toxicity and thus provide therapeutic benefit.Publication Open Access Exercise and caloric restriction improve cardiovascular and erectile function in rats with metabolic syndrome(2020-12-01) ŞENER, TARIK EMRE; ŞENER, GÖKSEL; Çevikelli Yakut Z. A., Özçelik R., Çevik Ö., Şener T. E., Şener G.The aim of this study is to examine the possible benefits of exercise and caloric restriction (CR) on cardiovascular hemodynamics, erectile function, and antioxidant system in metabolic syndrome (MS). Sixty male Spraque-Dawley rats were divided into five groups; control, MS, MS + CR, MS + exercise (EXC), and MS + CR + EXC. To induce MS, 10% fructose solution was applied for 3 months. Thereafter, in CR groups calorie was restricted 40% and in EXC groups swimming was performed for 6 weeks. Body weight, blood glucose, and blood pressure (BP) levels were measured before and after MS induction and at the end of the experiment. After decapitation, tumor necrosis factor (TNF)-α, adiponectin (ADP), and plasminogen activator inhibitor (PAI)-1 levels were investigated in blood, oxidative stress parameters were examined in heart, aorta, and corpus cavernosum (CC) tissues. Isometric contraction in isolated tissue bath was studied in aorta and CC tissues. Animals subjected to exercise and CR had decreased BP and blood glucose levels. Impaired contraction-relaxation responses in MS group were improved with exercise and CR. MS-induced increase in TNF-α, PAI-1, malondialdehyde (MDA), and decrease in ADP, glutathione (GSH), and superoxide dismutase (SOD) were normalized with exercise and CR. Exercise and CR may be beneficial against changes in cardiovascular hemodynamics caused by MS.Publication Metadata only Inhibitory effect of whey protein concentrate on SARS-CoV-2-targeted furin activity and spike protein-ACE2 binding in methotrexate-induced lung damage(2022) ÖZBEYLİ, DİLEK; Tufan, Elif; Sivas, Güzin Göksun; Gürel-Gökmen, Begüm; Yılmaz-Karaoğlu, Sümeyye; Ercan, Dursun; Özbeyli, Dilek; Şener, Göksel; Tunali-Akbay, TuğbaPublication Metadata only MYRTUS COMMUNIS IMPROVES COGNITIVE IMPAIRMENT IN RENOVASCULAR HYPERTENSIVE RATS(POLISH PHYSIOLOGICAL SOC, 2020) ŞEN, ALİ; Cevikelli-Yakut, Z. -A.; Ertas, B.; Sen, A.; Koyuncuoglu, T.; Yegen, B. C.; Sener, G.Myrtus communis has anti-inflammatory, neuroprotective and anticholinesterase activities yet there have been limited studies examining effects of Myrtus communis on cognitive functions. This study investigated the possible effects of Myrtus communis on changes in the cognitive functions of experimental renovascular hypertensive rats. Fifty-six Wistar-Albino rats were equally divided into 4 groups; sham-operated control, renovascular hypertension (RVH), ramipril (RVH + Ram) and Myrtus communis extract (RVH + MC) treatment groups. Goldblatt's 2-kidney 1-clip (2K1C) method was used to induce RVH. At the end of 9 weeks of treatment, after blood pressure recording, the animals underwent new object recognition test and Morris water maze (MWM) task. Following these tests, blood brain barrier (BBB) integrity was examined in 6 animals from each group. In the others after decapitation, osteopontin and interleukin (IL)-10 levels were measured in blood samples; while matrix metalloproteinase (MMP)-13, sodium potassium adenosine triphosphatase (Na+,K+-ATPase), cluster of differentiation (CD) 36, amyloid beta (Ab), neprilysin levels, and acetylcholinesterase (AChE) activity were investigated in hippocampal tissues. In RVH group, high systolic blood pressure decreased serum IL-10 levels, increased serum osteopontin levels and also impaired BBB permeability. Hippocampal MMP-13, CD36, Ab, neprilysin levels and AChE activities were elevated, while there were decreases in Na+,K+-ATPase levels. In new objet recognition test, discrimination index (DI) was determined as lower in saline-treated RVH group compared to control animals. In MWM training trail, 4th day performance in finding platform was significantly reduced in saline-treated RVH group compared to control group. RVH also decreased the time spent in target quadrant in probe test of MWM task compared to control group. In both of the treatment groups, all biochemical parameters were restored in parallel with improvement in the behavioral test performances. The results of this study suggest that Myrtus communis extract may improve the cognitive dysfunctions in hypertension through antihypertensive, anti-inflammatory and anticholinesterase activities.Publication Metadata only Myrtus communis leaf extract protects against cerulein-induced acute pancreatitis in rats(WILEY, 2020) ŞEN, ALİ; Ozbeyli, Dilek; Sen, Ali; Kaya, Ozlem Tugce Cilingir; Ertas, Busra; Aydemir, Sezgin; Ozkan, Naziye; Yuksel, Meral; Sener, GokselIn this study, the aim was to examine the potential protective effects of Myrtus communis subsp. communis leaf ethanol extract (MC) treatment against acute pancreatitis (AP) in rats. Thirty-two rats were grouped as the saline-pretreated control (C), MC-pretreated control (MC), saline-pretreated AP (AP), and MC-pretreated AP (MC + AP) groups. To induce AP, cerulein was administered (50 mu g/kg) two times. The rats were given MC for 14 days before cerulein injection. Six hours after the final cerulein injection, the rats were sacrificed. Pancreatic damage was associated with an increase in the serum activity of lipase and amylase, the pancreatic activity of myeloperoxidase, and the pancreatic level of malondialdehyde, interleukin-1 beta, and interleukin-6. AP also led to a decrease in the pancreatic level of anti-inflammatory interleukin-10 and glutathione. Pretreatment with MC before the induction of AP significantly reduced the pancreatic damage observed during the histological examination as well as reversed the biochemical changes evoked by AP. Practical applications Acute pancreatitis is characterized by high mortality (average about 5%; severe cases may reach about 30%). The current treatment for acute pancreatitis is mainly symptomatic. The introduction of herbal drugs may lead to the development of a new strategy in the treatment of this disease. This study revealed that MC reduced pancreatic injury by decreasing pro-inflammatory cytokines, increasing antioxidant capacity and anti-inflammatory cytokine, IL-10. To the authors' knowledge, this research is the first report showing that MC inhibits the development of AP. This observation suggests that MC may be useful in the prevention and the treatment of AP in clinical settings.Publication Open Access Effects of resveratrol against scattered radiation-induced testicular damage in rats(WALTER DE GRUYTER GMBH, 2021-09-06) ATASOY, BESTE MELEK; Sener, Tarik Emre; Atasoy, Beste Melek; Cevik, Ozge; Kaya, Ozlem Tugce Cilingir; Cetinel, Sule; Degerli, Ayse Dagli; Sener, GokselObjectives: To investigate the possible protective effects of resveratrol against oxidative testicular damage due to scattered radiation during pelvic ionizing radiation exposure in rats. Methods: Rats were divided into 5 groups; control, radiation, and radiation + resveratrol therapy in early and late periods. Under anesthesia, 20 Gy ionizing radiation was applied to prostatic region. Resveratrol was administered (10 mg/kg/day) orally before ionizing radiation exposure. Animals were decapitated at the end of 1st and 10th weeks. Biochemical markers of oxidative stress; caspase-3 and sirtuin-1 protein expressions; testosterone levels were evaluated, histological examinations were performed. Results: Significant increases in malondialdehyde, 8-hydroxy-deoxyguanosine levels, myeloperoxidase, and caspase-3 activities were observed after ionizing radiation exposure, also superoxide dismutase and glutathione activities were significantly decreased. Radiotherapy increased caspase-3 and decreased sirtuin-1 protein expressions. Resveratrol treatment significantly reversed these parameters and also reversed the decrease in testosterone levels back to control levels in late period. Conclusion: Resveratrol showed antioxidant and sirtuin-activating properties against oxidative damage caused by scattered radiation to testis and provided hormonal protection. These results suggest that resveratrol may be an alternative protective agent on testicular tissues against the effects of scattered pelvic radiation.
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