Person: AKBAY, TUĞBA
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Publication Metadata only Melatonin improves hyperglycemia induced damages in rat brain(WILEY, 2018) YARAT, AYŞEN; Gurel-Gokmen, Begum; Ipekci, Hazal; Oktay, Sehkar; Alev, Burcin; Ustundag, Unsal Veli; Ak, Esin; Akakin, Dilek; Sener, Goksel; Emekli-Alturfan, Ebru; Yarat, Aysen; Tunali-Akbay, TugbaBackground Diabetes mellitus is an endocrine disorder which is characterized by the development of resistance to the cellular activity of insulin or inadequate insulin production. It leads to hyperglycemia, prolonged inflammation, and oxidative stress. Oxidative stress is assumed to play an important role in the development of diabetic complications. Melatonin is the hormone that interacts with insulin in diabetes. Therefore, in this study, the effects of melatonin treatment with or without insulin were examined in diabetic rat brain. Methods Results Rats were divided into five groups as control, diabetes, diabetes + insulin, diabetes + melatonin, and diabetes + melatonin + insulin. Experimental diabetes was induced by streptozotocin (60 mg/kg, i.p.). Twelve weeks after diabetes induction, rats were decapitated. Malondialdehyde, glutathione, sialic acid and nitric oxide levels, superoxide dismutase, catalase, glutathione-S-transferase, myeloperoxidase, and tissue factor activities were determined in brain tissue. Melatonin alone showed its antioxidant effect by increasing brain glutathione level, superoxide dismutase, catalase, and glutathione-S-transferase activities and decreasing malondialdehyde level in experimental diabetes. Although insulin did not have a significant effect on glutathione and glutathione-S-transferase, its effects on lipid peroxidation, superoxide dismutase, and catalase were similar to melatonin; insulin also decreased myolopeoxidase activity and increased tissue factor activity. Combined melatonin and insulin treatment mimicked the effects of insulin. Conclusion Addition of melatonin to the insulin treatment did not change the effects of insulin, but the detailed role of melatonin alone in the treatment of diabetes merits further experimental and clinical investigation.Publication Metadata only Silymarin, the antioxidant component of Silybum marianum, protects against burn-induced oxidative skin injury(ELSEVIER SCI LTD, 2007) ERCAN, FERİHA; Toklu, Hale Z.; Tunah-Akbay, Tuba; Erkani, Gozde; Yuksel, Meral; Ercan, Feriha; Sener, GokselBackground: Despite recent advances, severe burn is one of the most common problems faced in the emergency room. Major thermal injury induces the activation of an inflammatory cascade resulting in local tissue damage, to contribute to the development of subsequent damage of multiple organs distant from the original burn wound. Objective: Silymarin, the major component of milk thistle has been shown to have antioxidant properties. In the present study, we investigated the putative antioxidant effect of local or systemic silymarin treatment on burn-induced oxidative tissue injury. Methods: Wistar albino rats were exposed to 90 degrees C bath for 10 s to induce burn. Silymarin either locally (30 mg/kg) applied on 4 cm(2) area or locally + systemically (50 mg/kg, p.o.) was administered after the burn and repeated twice daily. Rats were decapitated 48 h after injury and blood was collected for tumor necrosis factor-a (TNF-alpha) and lactate dehydrogenase (LDH) activity. In skin tissue samples malondialdehyde (MDA) and glutathione (GSH) levels, myeloperoxidase (MPO) activity, and luminol-lucigenin chemiluminescense (CL) were measured in addition to the histological evaluation. Results: Burn caused a significant increase in TNF-a and LDH levels. MDA levels were increased and GSH levels were decreased in the skin at 48 h after-burn. Both local and systemic silymarin treatments significantly reversed these parameters. The raised MPO activity and luminol-lucigenin CL were also significantly decreased. Conclusion: Results indicate that both systemic and local administration of silymarin was effective against burn-induced oxidative damage and morphological alterations in rat skin. Therefore, silymarin merits consideration as a therapeutic agent in the treatment of burns. (C) 2006 Elsevier Ltd and ISBI. All rights reserved.Publication Metadata only Effect of an Aqueous Garlic Extract on Kidney Damage in an Experimental Model of Sepsis(MARMARA UNIV, INST HEALTH SCIENCES, 2017) ŞENER, GÖKSEL; Ipekci, Hazal; Akbay, Tugba Tunali; Sener, GokselObjective: Sepsis is a systemic inflammatory response against pathogens or substances secreted by pathogens. In this study, the potential protective effect of an aqueous garlic extract (AGE) against sepsis-induced kidney injury. Methods: Rats were divided into four groups: control, sepsis, sepsis+AGE-garlic, and sepsis+pretreated garlic. Sepsis was induced using cecal ligation and perforation. An AGE was orally administered to rats in the sepsis+pretreated garlic group at a dose of 250 (mg/kg/day) for 15 days prior to sepsis induction. In rats in the sepsis+garlic group, the AGE was administered at a single dose (250 mg/kg) immediately after sepsis induction. Twelve hours after sepsis induction, all rats were decapitated and kidney tissues were taken. Glutathione (GSH) and malondialdehyde (MDA) levels and superoxide dismutase (SOD), tissue factor (TF), catalase (CAT), and myeloperoxidase (MPO) activities were determined in the kidney issue. Results: Increased MDA levels and MPO activity and decreased GSH level and SOD and CAT activities due to sepsis were reversed by the AGE. TF activity did not change in sepsis. Shortened clot formation time shows increased TF activity. Accordingly, kidney TF activity significantly increased in mice in the pre-treated garlic group. Conclusion: AGE usage should be considered in developing new sepsis treatment strategies in terms of oxidant and antioxidant balance.