Person: AKBAY, TUĞBA
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AKBAY
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TUĞBA
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Publication Metadata only Melatonin improves hyperglycemia induced damages in rat brain(WILEY, 2018) YARAT, AYŞEN; Gurel-Gokmen, Begum; Ipekci, Hazal; Oktay, Sehkar; Alev, Burcin; Ustundag, Unsal Veli; Ak, Esin; Akakin, Dilek; Sener, Goksel; Emekli-Alturfan, Ebru; Yarat, Aysen; Tunali-Akbay, TugbaBackground Diabetes mellitus is an endocrine disorder which is characterized by the development of resistance to the cellular activity of insulin or inadequate insulin production. It leads to hyperglycemia, prolonged inflammation, and oxidative stress. Oxidative stress is assumed to play an important role in the development of diabetic complications. Melatonin is the hormone that interacts with insulin in diabetes. Therefore, in this study, the effects of melatonin treatment with or without insulin were examined in diabetic rat brain. Methods Results Rats were divided into five groups as control, diabetes, diabetes + insulin, diabetes + melatonin, and diabetes + melatonin + insulin. Experimental diabetes was induced by streptozotocin (60 mg/kg, i.p.). Twelve weeks after diabetes induction, rats were decapitated. Malondialdehyde, glutathione, sialic acid and nitric oxide levels, superoxide dismutase, catalase, glutathione-S-transferase, myeloperoxidase, and tissue factor activities were determined in brain tissue. Melatonin alone showed its antioxidant effect by increasing brain glutathione level, superoxide dismutase, catalase, and glutathione-S-transferase activities and decreasing malondialdehyde level in experimental diabetes. Although insulin did not have a significant effect on glutathione and glutathione-S-transferase, its effects on lipid peroxidation, superoxide dismutase, and catalase were similar to melatonin; insulin also decreased myolopeoxidase activity and increased tissue factor activity. Combined melatonin and insulin treatment mimicked the effects of insulin. Conclusion Addition of melatonin to the insulin treatment did not change the effects of insulin, but the detailed role of melatonin alone in the treatment of diabetes merits further experimental and clinical investigation.Publication Metadata only Melatonin reduces oxidative damage to skin and normalizes blood coagulation in a rat model of thermal injury(PERGAMON-ELSEVIER SCIENCE LTD, 2005) YARAT, AYŞEN; Tunali, T; Sener, G; Yarat, A; Emekli, NThis study was designed to determine the effect of melatonin treatment on the glutathione (GSH) and lipid peroxidation (LPO) levels in the skin as well as prothrombin time (PT) and fibrin degradation products (FDPs) in the blood of rats with thermal injury. Under ether anaesthesia, the shaved dorsum of the rats was exposed to 90degreesC bath for 10 s to induce burn injury. Rats were decapitated either 3 or 24 hours after burn injury. Melatonin (10 mg/ kg) was administered i.p. immediately after burn injury to same animals. In the 24 hour burn group, melatonin injections were repeated for two more occasions 8 and 16 h after burn injury. In the control group the same protocol was applied except that the dorsum was exposed to a 25degreesC water bath for 10 s. Severe skin scald injury (30% of total body surface area) caused a significant decrease in PT at post burn 3 and 24 hours. FDPs was not increased at post burn 3 hour but was significantly increased at post burn 24 hour. GSH levels were significantly depressed at post burn 3 hour but were not changed at post burn 24 hour. LPO levels were significantly increased both at post burn 3 and 24 hours. Skin protein levels were significantly reduced at post burn 24 hour as evidenced by electrophoresis. Treatment of rats with melatonin normalized PT levels both at post burn 3 and 24 hours. FDP decreased at post burn 24 hour due to melatonin treatment. GSH levels significantly increased as a result of melatonin treatment both at post burn 3 and 24 hours melatonin treatment. LPO levels were not changed by melatonin at post burn 3 hour; however, the melatonin significantly decreased LPO values at post burn 24 hours. In conclusion, exogenously administered melatonin reduced skin oxidant damage and normalized the activated blood coagulation induced by thermal trauma. (C) 2004 Elsevier Inc. All rights reserved.Publication Metadata only Effect of an Aqueous Garlic Extract on Kidney Damage in an Experimental Model of Sepsis(MARMARA UNIV, INST HEALTH SCIENCES, 2017) ŞENER, GÖKSEL; Ipekci, Hazal; Akbay, Tugba Tunali; Sener, GokselObjective: Sepsis is a systemic inflammatory response against pathogens or substances secreted by pathogens. In this study, the potential protective effect of an aqueous garlic extract (AGE) against sepsis-induced kidney injury. Methods: Rats were divided into four groups: control, sepsis, sepsis+AGE-garlic, and sepsis+pretreated garlic. Sepsis was induced using cecal ligation and perforation. An AGE was orally administered to rats in the sepsis+pretreated garlic group at a dose of 250 (mg/kg/day) for 15 days prior to sepsis induction. In rats in the sepsis+garlic group, the AGE was administered at a single dose (250 mg/kg) immediately after sepsis induction. Twelve hours after sepsis induction, all rats were decapitated and kidney tissues were taken. Glutathione (GSH) and malondialdehyde (MDA) levels and superoxide dismutase (SOD), tissue factor (TF), catalase (CAT), and myeloperoxidase (MPO) activities were determined in the kidney issue. Results: Increased MDA levels and MPO activity and decreased GSH level and SOD and CAT activities due to sepsis were reversed by the AGE. TF activity did not change in sepsis. Shortened clot formation time shows increased TF activity. Accordingly, kidney TF activity significantly increased in mice in the pre-treated garlic group. Conclusion: AGE usage should be considered in developing new sepsis treatment strategies in terms of oxidant and antioxidant balance.