Person: AKBAY, TUĞBA
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AKBAY
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TUĞBA
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Publication Open Access Antiproliferative activity of whey and casein bioactive peptides on breast cancer: an in vitro and in silico study(2022-07-01) AKBAY, TUĞBA; Sahna K. O., Cakir B., Tunali-Akbay T.Bioactive peptides from milk proteins are dietary components with various biological properties and therapeutic effects in a variety of health conditions, including cancer. In this study, the effects of goat milk proteins and bioactive peptides on the MCF-7 breast cancer cell line were investigated. Milk was separated into casein and Whey fractions and treated with either pepsin or trypsin enzymes. Peptides of the hydrolyzed fractions were identified with LC-QTOFF/MS. After identification, the hydrolyzed Whey and casein fractions were incubated with MCF-7 breast cancer cells at various concentrations. The in vitro cytotoxicity of hydrolyzed fractions against MCF-7 cells was tested using MTT assay and flow cytometry analysis. The dead MCF-7 cells were analyzed with LC-Q-TOF/MS. In dead MCF-7 cells, the pyruvate kinase M2, mucin 1-C, glycogen synthase kinase 3-beta, and L-lactate dehydrogenase B were found to be downregulated in the LC-Q-TOF/MS analysis, while receptor-interacting serine/ threonine-protein kinase 1 was found to be up-regulated. These enzymes and mucin-1C also interacted with the milk-derived bioactive peptides in silico. The goat milk bioactive peptides exhibited high binding affinity with these enzymes and mucin-1C. The 5 mu g mL(-1) trypsin-treated casein fraction caused the highest MCF-7 cell death. In conclusion, the bioactive peptides of the pepsin-treated casein fraction and trypsin-treated Whey fraction of goat milk may inhibit the survival of breast cancer cells and exert their effects via multiple mechanisms. The results of this study are novel, suggesting that goat milk-based specific bioactive peptides may have potential as an anti-tumour agent in the treatment of breast cancer.Publication Open Access Lactose hydrolyzing activity of the lactase immobilized polycaprolactone and silk fibroin-based nanofiber and nitrocellulose membrane(2022-10-01) AKBAY, TUĞBA; Yılmaz-Karaoğlu S., Gürel-Gökmen B., AKBAY T.© 2022 Elsevier LtdThis study aimed to investigate the activity of lactase immobilized polycaprolactone and silk fibroin (PCL/SF)-based nanofiber and nitrocellulose membrane for the preparation of lactose-reduced milk. PCL/SF -based nanofiber was prepared by using the electrospinning method. The lactase enzyme was immobilized using the physical adsorption method on both surfaces, and then the optimum operating temperature and pH of the immobilized enzymes were determined. The efficiency of the immobilized lactase enzyme was determined in both goat and cow milk. The nutrient content of milk was also analyzed before and after the incubation of nanofiber and membrane with milk. The lactose hydrolysis efficiency of the lactase immobilized nitrocellulose membrane was found to be higher than the lactase immobilized PCL/SF -based nanofiber. Lactose was hydrolyzed 59% in cow milk and 87% in goat milk by using lactase immobilized nitrocellulose membrane. 42% of lactose was also hydrolyzed in cow milk and 21% was hydrolyzed in goat milk by using lactase immobilized PCL/SF-based nanofiber. However, the use of these two bioactive surfaces did not change the fat and protein composition of both cow and goat milk. In conclusion, lactase immobilized nitrocellulose membrane was found to be more advantageous in the production of lactose-reduced milk than the lactase immobilized polycaprolactone/silk fibroin nanofiber.Publication Open Access Morphological and biochemical investigation of the protective effects of panax ginseng on methotrexate-induced testicular damage(2023-06-01) AKBAY, TUĞBA; ERCAN, FERİHA; Karakaya F. B., Macit Ç., Sivas G. G., Akbay T., Şeber G., Ercan F.Objective: Methotrexate (MTX) is a chemotherapeutic agent that causes testicular toxicity used in the cure of various types ofcancer. The anti-oxidant and anti-cancer effects of Panax ginseng (PxG) have been reported in both experimental and clinical studies. This study aims to examine the healing effect of PxG on testicular damage induced by MTX.Materials and Methods: Sprague Dawley male rats (8-week-olds) were used in the study. A single dose ofMTXdissolved in saline(20 mg/kg) was given to MTX and MTX+PxG groups by intraperitoneal injection. PxG dissolved in saline (100 mg/kg) was given by orogastric gavage once a day for 5 days to the MTX+PxG group. Saline was given to the control and MTX groups orally during the experiments. After decapitation, the testis sampleswere obtained. Seminiferous tubules and basement membranewere evaluated histopathologically. Seminiferous tubule diameter and germinal epithelium thickness were measured. Furthermore, oxidative stress parameters such as malondialdehyde, glutathione, superoxide dismutase, and glutathione-S-transferase were measured.Results: MTX treatment caused seminiferous tubule degeneration with a decrease in Johnsen’s score, the seminiferous tubule’sdiameter, and the germinal epithelium’s thickness. Parallel with the histopathological results increased testicular oxidative stress with an increase in malondialdehyde level and a decrease of endogenous anti-oxidant activity with a decrease in glutathione level and glutathione-S-transferase and superoxide dismutase activities. PxG treatment improved these histological and biochemical parameters in MTX-induced testis cytotoxicity.Conclusion: MTX treatment causes testicular damage via the oxidative processes. PxG treatment ameliorates MTX-inducedtesticular damage by inhibiting oxidative stress.Publication Open Access Myrtle improves renovascular hypertension-induced oxidative damage in heart, kidney, and aortic tissue(2022-07-01) ŞEN, ALİ; ŞENER, GÖKSEL; AKBAY, TUĞBA; Arslan S., Ozcan O., Gurel-Gokmen B., ÇEVİKELLİ YAKUT Z. A., Saygi H. I., ŞEN A., GÖGER F., ERKANLI ŞENTÜRK G., Sener G., Tunali-Akbay T.Renovascular hypertension is defined as the reduction in renal perfusion resulting in sustained hypertension. This study aims to investigate the possible effects of myrtle leaf extract (Myrtus communis L.) on, heart, kidney and aorta tissues in the experimental renovascular hypertension (RVH). 32 male Wistar Albino rats were divided into four groups as control, hypertension, hypertension+ramipril, and hypertension+myrtle leaf extract treatment groups. RVH model was induced by Goldblatt\"s 2-kidney 1-clip method. 12 weeks later than the treatment blood pressures were recorded and oxidant and antioxidant parameters, tissue factor activity, and histological analysis were determined in the kidney, heart, and aortic tissues. The blood pressure levels of the hypertension group significantly increased compared to the control group. Administration of myrtle leaf extract and ramipril significantly decreased the increased blood pressure. In the hypertension group, oxidative damage increased in the kidney, heart, and aorta tissues. In the histological evaluation of tissues in RVH, heart muscle fibres degenerated. Bowman capsule and glomeruli dilated and tubules damaged in the kidney. Myrtle leaf extract administration regenerated the damages and degenerations. The administration of myrtle leaf extract restored the impaired oxidant-antioxidant balance in the heart, kidney and aorta tissues of hypertensive rats. Myrtle leaf extract can be considered as an alternative antihypertensive treatment target in the prevention of oxidative stress-induced damage in renovascular hypertension.Publication Open Access Brain in metabolic syndrome model: The effect of exercises and caloric restriction(2022-01-01) AKBAY, TUĞBA; ALTURFAN, EBRU IŞIK; ŞENER, GÖKSEL; YARAT, AYŞEN; Alev-Tuzuner B., Genc-Kahraman N., Ipekci H., Ustundag U. V. , AKBAY T., ALTURFAN E. I. , Sener G., YARAT A.© 2022 Marmara University Press.Caloric restriction (CR) and exercise (EX) have impacts on improving metabolic risk factors. This study aimed to investigate the changes in the brain after EX and/or CR in metabolic syndrome (MeS) induced by a high fructose diet in rats. Sprague-Dawley male rats were divided into five groups. Drinking water including 10% fructose solution was given to rats for 12 weeks to develop a MeS rat model. Animals with MeS were submitted to EX and/or CR for 6 weeks. Blood glucose, and brain tissue damage and antioxidant parameters were measured. Brain lipid peroxidation, sialic acid, mucin, fucose levels increased in the MeS group compared to the control (C) group. These parameters reduced significantly in the metabolic syndrome with caloric restriction (MeS+CR) group, and more significantly in the metabolic syndrome with exercise and caloric restriction group (MeS+EXCR), compared to the MeS group. Glutathione levels, superoxide dismutase and catalase activities decreased in the MeS group compared to the C group, increased both in the MeS+CR group, and MeS+EXCR group compared to the MeS group. High fructose diet consumption can lead to brain tissue damage and decreased antioxidant levels were found to be improved best in the MeS+EXCR group.