Person: DOĞAN, AHMET
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DOĞAN
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AHMET
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Publication Metadata only Chemical composition and bio-functional perspectives of Erica arborea L. extracts obtained by different extraction techniques: Innovative insights(ELSEVIER, 2019) DOĞAN, AHMET; Zengin, Gokhan; Cvetanovic, Aleksandra; Gasic, Uros; Stupar, Alena; Bulut, Gizem; Senkardes, Ismail; Dogan, Ahmet; Seebaluck-Sandoram, Roumita; Rengasamy, Kannan R. R.; Sinan, Kouadio Ibrahime; Mahomoodally, Mohamad FawziErica arborea L., also known as Estrella Gold, is traditionally used for several purposes. In this research, five different extraction techniques: accelerated solvent extraction (ASE), microwave-assisted extraction (MAE), maceration (MAC), soxhlet (SOE) and ultrasound-assisted extraction (UAE) were used to compare the total phenolic, flavonoids, total antioxidant activity and enzymatic activities of E. arborea extracts obtained different extraction techniques. The total phenolic and flavonoid contents were in the order of ASE > MAE > SOE > MAC > UAE. All extracts showed antioxidant, anticholinesterase, anti-tyrosinase and anti-diabetic activities. A highly sensitive method using ultra-high-pressure liquid chromatography coupled with linear ion trap-Orbitrap tandem mass spectrometry (UHPLC-LTQ-Orbitrap-MS) has been used for the qualitative analysis of obtained extracts. Seventy-two polyphenolic compounds were identified in all extracts. However, 20 components were quantified among the extracts. ASE was found to be a better extraction technique as compared to the other extraction techniques. E. arborea can be exploited in the discovery of bioactive natural products for the treatment of Alzheimer's disease, diabetes and pigmentation problems.Publication Metadata only HPLC-MS/MS-based metabolic profiling and pharmacological properties of extracts and infusion obtained from Amelanchier parviflora var. dentata(ELSEVIER SCIENCE BV, 2018) DOĞAN, AHMET; Zengin, Gokhan; Diuzheva, Alina; Jeko, Jozsef; Cziaky, Zoltan; Bulut, Gizem; Dogan, Ahmet; Haznedaroglu, Mehmet Zeki; Rengasamy, Kannan R. R.; Lobine, Devina; Bahadori, Mir Babak; Mahomoodally, Mohamad FawziArnelanchierparviflora var. dentata is an endemic medicinal plant traditionally consumed to treat stomach pain, cardiovascular diseases, and urinary tract infections in Turkey's folk medicine. Although Amelanchier genus is an excellent source of phytochemicals with a panoply of biological activities, no report has been made available on Amelanchier parviflora var. dentata. Thus, the metabolic profiles of methanol, ethyl acetate, and water extracts were obtained using HPLC-MS/MS. Besides, the pharmacological properties such as antioxidant activity and enzyme inhibition effects against acetyl/butyrylcholinesterase, tyrosinase, alpha-amylase, and alpha-glucosidase were evaluated. In total, 62, 38, and 16 metabolites were Identified from the methanolic, water, and ethyl acetate extracts, respectively. The identified metabolites belong to anthocyanins, phenolic acids, and flavonoids. The tested extracts exhibited different enzyme inhibitory activity against cholinesterases (acetylcholinesterase: 0.52-2.47 mg GEs/g extract and butyrylcholinesterase: 0.47-3.59 mg GEs/g extract), tyrosinase (19,0-145.54 mg KAEs/g extract), alpha-amylase (0.23-1.10 mmol ACEs/g extract), and alpha-glucosidase (8.89-9.92 mmol ACEs/ g extract). Evidence from the present study supports the necessity for more research in an endeavor to discover biologically active secondary metabolites from A. parviflora to manage specific ailments.Publication Open Access An Ethnobotanical study of medicinal plants in Taşköprü (Kastamonu–Turkey)(2022-10-20) ŞENKARDEŞ, İSMAİL; DOĞAN, AHMET; EMRE, GİZEM; ŞENKARDEŞ İ., DOĞAN A., EMRE G.Copyright © 2022 Senkardes, Dogan and Emre.This pharmaceutical ethnobotanical research was carried out in Taşköprü District in Kastamonu, in northern Anatolia. It assembles the elaborations of plants used as folk medicines, and the ethnopharmacological data collected in the course of in-person semi-structured interviews with an open-ended questionnaire. The study’s aims were two-fold: gathering and identifying plants that the local inhabitants use therapeutically, and recording information related to traditional folk medicine (primarily for humans, and if extant for animals). The plants were gathered during several outings between May 2016 and July 2018. The organization of the data was based on the use-reports (UR) and was done according to the ICPC-2 classification. In addition, cultural importance index (CI) and informant consensus factor (FIC) calculations were made for the data collected. The research identified 101 plant taxa of 31 families used in folk medicine. Of these, 89 were wild and 12 were cultivated taxa. In total, 499 medicinal uses were determined. The CI values indicated that the most significant medicinal plant specimens were Pinus nigra subsp. pallasiana (0.78), P. sylvestris var. hamata (0.75) and Plantago lanceolata, P. major subsp. intermedia and P. major subsp. major (0.58 each). The most prevalent families were Asteraceae (2.14), Rosaceae (1.93), Pinaceae (1.81) and Plantaginaceae (1.74). Respiratory system (0.95), skin and subcutaneous tissue (0.94), nervous system (0.92) and circulatory system disorders (0.88) and ethnoveterinary uses (0.89) had the highest FIC values. The most frequently used preparation process was observed to be decoction (38.4%) and the most commonly utilized plant parts were aerial (21%). Along with recording 20 plant taxa as medicinal plants for the first time, this study documented a total of 303 new therapeutic uses. This study concludes with the finding that traditional knowledge of medicinal plants remains prevalent in Ta rticularly among its rural inhabitants.Publication Open Access An Ethnobotanical Study of Medicinal Plants in Mersin (Turkey)(FRONTIERS MEDIA SA, 2021-07-07) DOĞAN, AHMET; Emre, Gizem; Dogan, Ahmet; Haznedaroglu, Mehmet Zeki; Senkardes, Ismail; Ulger, Mahmut; Satiroglu, Aysen; Emmez, Berivan Can; Tugay, OsmanThis comprehensive ethnobotanical study carried out in Mersin province, which is located in the southern part of Anatolia, east of the Mediterranean Sea, compiles details on plants used in folk medicine and ethnopharmacological information obtained through face-to-face interviews. The aim was to collect and identify plants used for therapeutic purposes by local people and to record information on traditional herbal medicine. Plant specimens were collected in numerous excursions. Additionally, informant consensus factor and use value (UV) were calculated for information gathered. This study identifies 93 plant taxa belonging to 43 families and records their usage in folk medicine; 83 taxa are wild and the remaining 10 are cultivated. The most commonly used plants belong to Lamiaceae, representing 15.0% of the total, while the Rosaceae, Malvaceae, Hypericaceae, Asteraceae and Cupressaceae families each represented another 5.4%. As a result of this investigation, we determine 189 medicinal usages of 93 taxa. The UV values indicate that the most important medicine plants are Hypericum perforatum (0.80), Cedrus libani (0.78), Quercus coccifera (0.77), Arum dioscoridis (0.76) and Juniperus drupaceae (0.74). We observed that most of the drugs are prepared using the infusion method (27.6%). As a conclusion, the study finds that traditional folk medicine usage is still common, especially among the rural population of Mersin.Publication Metadata only Paeonia arietina and Paeonia kesrounansis bioactive constituents: NMR, LC-DAD-MS fingerprinting and in vitro assays(ELSEVIER SCIENCE BV, 2019) DOĞAN, AHMET; Sut, Stefania; Zengin, Gokhan; Dall'Acqua, Stefano; Gazdova, Marketa; Smejkal, Karel; Bulut, Gizem; Dogan, Ahmet; Haznedaroglu, Mehmet Zeki; Aumeeruddy, Muhammad Zakariyyah; Maggi, Filippo; Mahomoodally, Mohamad FawziPaeonia species have been valued for their ethnomedicinal uses in various countries and received much interest among the scientific community for their therapeutic properties, including anti-microbial, anti-inflammatory, anti-cancer, nephroprotective and hepatoprotective effects. The multiple phytother-apeutical applications of Paeonia species inspired us to establish the phytochemical fingerprint and to evaluate the biological properties of ethyl acetate, methanol, and aqueous extracts from the roots and aerial parts of two Paeonia species (P. arietina G. Anderson and P. kesrounansis Thiebaut). Phytoconstituents of P. arietina and P. kesrounansis extracts were analyzed using 1D and 2D NMR and LC-DAD-ESI-MS. The total content of phenolics (TPC) and flavonoids (TFC) in the extracts was also evaluated. The antioxidant activity was profiled using DPPH, ABTS, CUPRAC, FRAP, phosphomolybdenum, and metal chelation assays. Enzyme inhibitory properties were evaluated against acetylcholinesterase (AChE), butyrylcholinesterase (BChE), tyrosinase, alpha-amylase, and alpha-glucosidase. Phytochemical analysis of P. arietina and P. kesrounansis extracts showed the presence of galloyl esters of sugars, galloyl monoterpenes, and glycosylated flavonoids. The three solvent extracts presented different behavior in the bioassays. The highest antioxidant activity, tyrosinase and AChE inhibition were observed for the methanolic extract of the aerial parts of P. kesrounansis. In addition, the ethyl acetate extracts of the aerial parts of both plants were the most effective inhibitors of alpha-amylase. The highest BChE inhibition was observed for root methanolic extract of P. kesrounansis while the root ethyl acetate extract of P. arietina exerted the strongest inhibition of alpha-glucosidase. Methanol extract of P. kesrounansis aerial parts presented the highest TPC, while TFC was greatest in the corresponding extract of P. arietina. Our findings can be considered as a starting point for future studies to further validate the effectiveness and safety profiles of these plants in folk medicine. (C) 2018 Elsevier B.V. All rights reserved.Publication Metadata only Modern and traditional extraction techniques affect chemical composition and bioactivity of Tanacetum parthenium (L.) Sch.Bip(ELSEVIER, 2020) DOĞAN, AHMET; Zengin, Gokhan; Cvetanovic, Aleksandra; Gasic, Uros; Stupar, Alena; Bulut, Gizem; Senkardes, Ismail; Dogan, Ahmet; Sinan, Kouadio Ibrahime; Uysal, Sengul; Aumeeruddy-Elalfi, Zaahira; Aktumsek, Abdurrahman; Mahomoodally, Mohamad FawziTanacetum parthenium (L.) Sch.Bip (TP), a flowering plant, is famous in traditional medicine to prevent migraine and headache. However, there is currently a dearth of studies to advocate the phytochemical profile and biological propensities of extracts prepared from this plant. This study endeavors to highlight the biological potential of TP extracts prepared by modern (ultrasound-UAE, microwave-MAE and accelerated-ASE extractions) and traditional (maceration and Soxhlet) extraction techniques. The chemical profile of the extracts was established via ultra-high-pressure liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) technique. Sixty different polyphenolic compounds belonging to the classes of phenolic acids, flavonoid glycosides and flavonoid aglycones were recorded in the extracts. Additionally, the quantity of 17 components was measured using appropriate standards and it was found that the modern extraction gave extracts with the higher content of observed compounds than the traditional techniques. Evaluation of antioxidant activity was determined in vitro via five standard assays. The inhibitory potential of TP extracts against key enzymes implicated in the noncommunicable diseases such as diabetes (alpha-amylase and alpha-glucosidase), neurodegenerative diseases (acetyl- and butyrylcholinesterase) and skin diseases (tyrosinase), was assessed. Potent antioxidant ability of all TP extract was revealed with a predominance for the extracts yielded using the ASE method. This potent antioxidant activity of the extracts corroborated with the high phenolic (65.05 +/- 0.27 mg gallic acid equivalent (GAE)/g extract) and flavonoid contents (55A0 +/- 0.52 mg rutin equivalent (RE)/g extract). Tanacetum partheniwn extracts also showed significant alpha-glucosidase inhibitory activity (1.63-1.67 mmol acarbose equivalent (ACAE)/g extract) and moderate inhibition activity against a-amylase (0.51-0.56 mmol ACAE/g extract). The extracts also showed potent activity against cholinesterases and tyrosinase. This study tend to validate the use of TP extracts obtained by novel extraction techniques such as ASE, as potent bioactive extracts to be further studied for therapeutic bio-product development.Publication Metadata only New insights into the in vitro biological effects, in silico docking and chemical profile of clary sage - Salvia sclarea L.(ELSEVIER SCI LTD, 2018) DOĞAN, AHMET; Zengin, Gokhan; Senkardes, Ismail; Mollica, Adriano; Picot-Allain, Carene Marie Nancy; Bulut, Gizem; Dogan, Ahmet; Mahomoodally, M. FawziSalvia sclarea L. is traditionally used to manage common human ailments and is consumed as a food product. This study aimed to establish the phytochemical profile and antioxidant potential of ethyl acetate, methanol, and water extracts of Salvia sclarea. The inhibitory action of the extracts against alpha-amylase, alpha-glucosidase, acetyicholinesterase, butyrylcholinesterase, and tyrosinase was also investigated. Methanol extract showed the highest phenolic and fiavonoid contents (81.78 mg GAE/g extract and 40.59 mg RE/g extract, respectively). Reversed phase high performance liquid chromatography with diode array detector analysis revealed that S. sclarea was rich in rosmarinic acid. The water extract exhibited the lowest inhibitory activity against alpha-amylase but the upmost activity against alpha-glucosidase (0.19 and 18.24 mmol ACAE/g extract, respectively). Experimental data showed that only the water extract (8.86 mg KAE/g extract) significantly inhibited tyrosinase. Docking studies showed that quercetin binds to tyrosinase by two hydrogen and a pi-pi bonds. Salvia sclarea showed interesting biological activity against key enzymes involved in the pathogenesis of common ailments. (C) 2018 Elsevier Ltd. All rights reserved.Publication Metadata only Biologically active compounds from two members of the Asteraceae family: Tragopogon dubius Scop. and Tussilago farfara L.(TAYLOR & FRANCIS INC, 2019) DOĞAN, AHMET; Uysal, Sengul; Senkardes, Ismail; Mollica, Adriano; Zengin, Gokhan; Bulut, Gizem; Dogan, Ahmet; Glamoclija, Jasmina; Sokovic, Marina; Lobine, Devina; Mahomoodally, Fawzi M.Tragopogon dubius and Tussilago farfara are consumed as vegetables and used in folk medicine to manage common diseases. Herein, the chemical compositions and biological activities of different leaf extracts (ethyl acetate, methanol, and water) of T. dubius and T. farfara were evaluated. The antibacterial, antifungal, and antioxidant abilities of the extracts were tested using different assays including free radical scavenging, reducing power, phosphomolybdenum, and metal chelating assays. Enzyme inhibitory potentials were evaluated against cholinesterases, tyrosinase, alpha-amylase and alpha-glucosidase. Complexes of bioactive compounds (chlorogenic and rosmarinic acid) were docked into the enzymatic cavity of alpha-glucosidase and subjected to molecular dynamic calculation, enzyme conformational stability, and flexibility analysis. T. dubius and T. farfara extracts showed remarkable antioxidant potentials. Ethyl acetate extracts of T. dubius and T. farfara were the most potent inhibitors of acetylcholinesterase and butyrylcholinesterase. T. dubius ethyl acetate extract and T. farfara methanolic extract showed noteworthy activity against alpha-glucosidase. High performance liquid chromatography analysis revealed the abundance of some phenolic compounds including chlorogenic and rosmarinic acids. Ethyl acetate extract of T. dubius showed notable antifungal activity against all strains. Docking studies showed best pose for chlorogenic acid was stabilized by a network of hydrogen bonds with residues Asp1157, Asp1279, whereas rosmarinic acid showed several hydrogen bonds with Asp1157, Asp1420, Asp1526, Lys1460 and Trp1369. This study further validates the use of T. dubius and T. farfara in traditional medicine, as well as act as a stimulus for further studies for future biomedicine development. Communicated by Ramaswamy H. SarmaPublication Metadata only Influence of different extraction techniques on the chemical profile and biological properties of Anthemis cotula L.: Multifunctional aspects for potential pharmaceutical applications(ELSEVIER SCIENCE BV, 2019) DOĞAN, AHMET; Sut, Stefania; Dall'Acqua, Stefano; Zengin, Gokhan; Senkardes, Ismail; Bulut, Gizem; Cvetanovic, Aleksandra; Stupar, Alena; Mandic, Anamarija; Picot-Allain, Carene; Dogan, Ahmet; Sinan, Kouadio Ibrahime; Mahomoodally, FawziThe phytochemical composition of different extracts obtained from stinking chamomile (Anthemis cotula L) was investigated. Ethanol was used as solvent and accelerated solvent extraction (ASE), microwave assisted extraction (MAE), maceration, soxhlet extraction (SE), and ultrasound assisted extraction (UAE) were applied on plant material. Comparison of the phytochemical contents, antioxidant, and enzyme inhibitory properties were performed. The most abundant sesquiterpene in the extracts was anthecotuloide, while the most present phenolics were caffeoyl quinic acid, quercetin, and kaempferol derivatives. UAE extract was the most efficient in the extraction of sesquiterpenoids and polyphenols. Considering the assays on antioxidant activity and enzyme inhibition, ASE extract showed highest phenolic content (62.92 mg gallic acid equivalent/g extract). Likewise, this extract showed highest radical scavenging (103.44 mg trolox equivalent [TE]/g extract and 155.70 mg TE/g extract, for DPPH and ABTS assays respectively) and reducing power potential (435.32 and 317.89 mg TE/g extract, for CUPRAC and FRAP assays, respectively). The different extracts showed similar results in the enzyme inhibition assays suggesting that the extraction methods used have no effect on observed enzyme activities. Novelty of our findings are the inhibitory action of the ethanol extract of A. cotula aerial parts on key enzymes associated with Alzheimer's disease (acetyl cholinesterase, butyryl cholinesterase), type 2 diabetes (alpha-amylase, alpha-glucosidase), and skin hyperpigmentation disorders (tyrosinase). Data collected from the present work further appraises the multiple potential biological properties of stinking chamomile suggesting the need for further investigation on its constituents. (C) 2019 Elsevier B.V. All rights reserved.Publication Open Access Bioactivities of Achillea phrygia and Bupleurum croceum based on the composition of phenolic compounds: In vitro and in silico approaches(2017-09-01) EMRE, GİZEM; DOĞAN, AHMET; Zengin G., EMRE G., Mollica A., Haznedaroglu M. Z. , DOĞAN A., Aktumsek A.This study presents the effects of the Achillea phrygia Boiss. et Bal. and Bupleurum croceum Fenzl. extracts obtained by different solvents (ethyl acetate, methanol and water) on selected enzyme inhibitory effects and antioxidant ability with screening bioactive compounds. Total and individual bioactive compounds were detected by spectrophotometric and HPLC-DAD techniques. Antioxidant abilities were evaluated by different methods including free radical scavenging (ABTS and DPPH), reducing power (CUPRAC and FRAP), phosphomolybdenum and metal chelating. Enzyme inhibitory effects were tested against cholinesterases, tyrosinase, amylase, glucosidase and lipase. Total phenolic contents were ranged from 20.52 mgGAE/g extract (B. croceum methanol extract) to 41.13 mgGAE/g extract (A. phrygia methanol extract). Generally, methanol and water extracts showed the strongest antioxidant abilities, while the ethyl acetate extracts had the most promising enzyme inhibitory effects. HPLC analysis revealed the abundance of some phenolics including rutin, quercetin, sinapic acid and chlorogenic acid, respectively. These components were also assessed using molecular modelling with the aim to study their docking properties on a set of six enzymes used in this study. Overall, these species could be suggested as valuable sources of natural-bioactive agents for developing new functional, pharmacological and health promoting ingredients. (C) 2017 Elsevier Ltd. All rights reserved.