Person: DOĞAN, AHMET
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DOĞAN
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AHMET
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Publication Metadata only Characterization of Phenolic Constituents and Pharmacological Activity of Achillea vermicularis(INDIAN PHARMACEUTICAL ASSOC, 2019) DOĞAN, AHMET; Taskin, T.; Balkan, I. E.; Tankin, D.; Dogan, A.The aim of the present study was to compare for the first time, the antiinflammatory, antioxidant and antiurease activities of 15 different extracts of Achillea vermicularis, which were obtained from three extraction methods using five different solvents. Phenolic contents in chloroform extract showed strong antiinflammatory and antioxidant activity and were analysed by high-performance liquid chromatography with diode-array detection and confirmed by liquid chromatography quadrupole time-of-flight mass spectrometry. The active phenolic compounds in chloroform extracts were identified as chlorogenic acid, caffeic acid, rutin, dicaffeoylquinic acid, naringenin, quercetagetin 3,6-dimethyl ether, and 8-hydroxysalvigenin. All these compounds except caffeic acid were detected for the first time in Achillea vermicularis. These results indicated that chloroform extract of the plant possessed a high potential to be used as a natural source in food and pharmaceutical industry.Publication Open Access In vitro biological activities of different extracts from alcea dissecta(2022-03-01) TAŞKIN, TURGUT; DOĞAN, AHMET; TAŞKIN T., Kahvecioglu D., Turkoglu E. A., DOĞAN A., Kuzu M.Objective: Alcea genus belongs to Malvaceae family and this genus is represented by 85 taxa in the world and 21 taxa in the Flora of Turkey. The flowers of Alcea genus contain plenty of mucilage and are used for the treatment of respiratory diseases such as asthma and cough. Alcea dissecta is known as ‘Govik, Hiro, Hero’ in Turkey, and the flowers and leaves of this species have been used in the treatment of asthma, injury, colds, and gastrointestinal diseases in Turkey. To the best of our knowledge, there is no report on the effect of extraction methods on the biological activity of this plant. In addition, although this species is being used as a medical plant, there is no study of the antioxidant, antiurease, esterase, and anticholinesterase activity of the plant. Therefore, the aim of this study was to evaluate in vitro antioxidant, anti-urease, esterase, anticholinesterase activities of Alcea dissecta using a variety of extracts. Methods: The antioxidant activities of different extracts were examined by DPPH, ABTS, FRAP, and CUPRAC methods. The total phenolic compounds contained in the extracts were determined using the Folin-Ciocalteu reagent (FCR) method. Anti-urease and anticholinesterase activities of different extracts were evaluated by indophenol and Ellman methods respectively. In addition, esterase activities of plant extracts were determined. Results: In the present study, ethanol:water (1:1, v/v) and chloroform extracts obtained maceration method showed stronger DPPH and ABTS radical scavenging activity than other extracts. The chloroform extract obtained Soxhlet method was found to have higher FRAP and CUPRAC values than other extracts. It was also found that the ethanol extract obtained maceration method showed the most potent anti-urease and anticholinesterase activity. According to the results, the strongest inhibitory effect on both hCA I and II isoenzymes was shown by the petroleum ether extract obtained Soxhlet method. Conclusion: As a result, it was determined that different plant extracts have antioxidant, anti-urease, esterase, anticholinesterase activities. In addition, the data obtained from this study will shed light on future research on the biological activities of this species.