Person: DOĞAN, AHMET
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DOĞAN
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AHMET
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Publication Open Access An Ethnobotanical study of medicinal plants in Taşköprü (Kastamonu–Turkey)(2022-10-20) ŞENKARDEŞ, İSMAİL; DOĞAN, AHMET; EMRE, GİZEM; ŞENKARDEŞ İ., DOĞAN A., EMRE G.Copyright © 2022 Senkardes, Dogan and Emre.This pharmaceutical ethnobotanical research was carried out in Taşköprü District in Kastamonu, in northern Anatolia. It assembles the elaborations of plants used as folk medicines, and the ethnopharmacological data collected in the course of in-person semi-structured interviews with an open-ended questionnaire. The study’s aims were two-fold: gathering and identifying plants that the local inhabitants use therapeutically, and recording information related to traditional folk medicine (primarily for humans, and if extant for animals). The plants were gathered during several outings between May 2016 and July 2018. The organization of the data was based on the use-reports (UR) and was done according to the ICPC-2 classification. In addition, cultural importance index (CI) and informant consensus factor (FIC) calculations were made for the data collected. The research identified 101 plant taxa of 31 families used in folk medicine. Of these, 89 were wild and 12 were cultivated taxa. In total, 499 medicinal uses were determined. The CI values indicated that the most significant medicinal plant specimens were Pinus nigra subsp. pallasiana (0.78), P. sylvestris var. hamata (0.75) and Plantago lanceolata, P. major subsp. intermedia and P. major subsp. major (0.58 each). The most prevalent families were Asteraceae (2.14), Rosaceae (1.93), Pinaceae (1.81) and Plantaginaceae (1.74). Respiratory system (0.95), skin and subcutaneous tissue (0.94), nervous system (0.92) and circulatory system disorders (0.88) and ethnoveterinary uses (0.89) had the highest FIC values. The most frequently used preparation process was observed to be decoction (38.4%) and the most commonly utilized plant parts were aerial (21%). Along with recording 20 plant taxa as medicinal plants for the first time, this study documented a total of 303 new therapeutic uses. This study concludes with the finding that traditional knowledge of medicinal plants remains prevalent in Ta rticularly among its rural inhabitants.Publication Open Access Protective effects of Rubus tereticaulis leaves ethanol extract on rats with ulcerative colitis and bio-guided isolation of its active compounds: A combined in silico, in vitro and in vivo study(2022-11-01) ŞEN, ALİ; ÖZBEYLİ, DİLEK; ERTAŞ, BÜŞRA; DOĞAN, AHMET; BİTİŞ, LEYLA; Şen A., Özbeyli D., Teralı K., Göger F., Yıldırım A., Ertaş B., Doğan A., Bitiş L., Şener G.The aim of this study was to evaluate the therapeutic effect of active ethanol extract obtained from the leaves of Rubus tereticaulis (RTME) against colitis, and to purify major compounds from this extract by bioassay-directed isolation. Rats with colitis induced via intra-rectal acetic acid administration (5%, v/v) received RTME or sulfasalazine for three consecutive days. On day four, all rats were decapitated, and the colonic tissue samples were collected for macroscopic score, colon weight, reduced glutathione (GSH), myeloperoxidase (MPO), and malondialdehyde (MDA) analyses. The active compounds and chemical composition of RTME were determined by bio-guided isolation and LC-MS/MS, respectively. Compared to the colitis group, the rats treated with RTME displayed significantly lowered macroscopic scores and colon wet weights (p < 0.001). These effects were confirmed biochemically by a decrease in colonic MPO activity (p < 0.001), MDA levels (p < 0.001), and an increase in GSH levels (p < 0.001). Kaempferol-3-O-β-d-glucuronide (RT1) and quercetin-3-O-β-d-glucuronide (RT2) were found to be the major compounds of RTME, as evidenced by in vitro anti-inflammatory and antioxidant activity-guided isolation. Their anti-inflammatory/antioxidant activities were also predicted by docking simulations. Additionally, quinic acid, 5-caffeoylquinic acid, quercetin pentoside, quercetin glucoside, quercetin-3-O-β-d-glucuronide, kaempferol-3-O-β-d-glucuronide, and kaempferol rutinoside were identified in RTME via using LC-MS/MS. RT2, along with other compounds, may be responsible for the observed protective action of RTME against colitis. This study represents the first report on the beneficial effects of RTME in an experimental model of colitis and highlights the potential future use of RTME as a natural alternative to alleviate colitis.Publication Open Access Antioxidant, Enzyme Inhibitory and Calcium Oxalate Anti-crystallization Activities of Equisetum telmateia Ehrn.(2020-09-15) TAŞKIN, TURGUT; DOĞAN, AHMET; TAŞKIN T., YILMAZ B. N., DOĞAN A.Equisetum L. is the only genus of the Equisetaceae family, which commonly known as horsetails, in English and atkuyruğu or kırkkilit in Turkish. In traditional medicine, Equisetum telmateia Ehrh. is used in diseases of the urinary system, such as pyelonephritis, prostatic hypertrophy, and cystitis. Besides, this species is known to be used by humans to treat kidney stones or kidney sand. The extracts were obtained from the aerial parts of the E. telmateia using three different extraction methods (maceration, Soxhlet, ultrasonic bath) and their antioxidant (ABTS, CUPRAC), anti-urease and anticholinesterase activities were examined. Also, calcium oxalate anti-crystallization activity of Soxhlet methanol extract showing strong antioxidant activity was determined. Soxhlet methanol extract exhibited stronger ABTS radical scavenging (0.0676 mM Trolox/mg extract) and cupric ion reducing/antioxidant (4.351 mM Trolox/mg extract) activity than other extracts. Soxhlet methanol (65.528%) and maceration methanol (61.965%) extracts showed the strongest anticholinesterase activity. In the anti-urease assay, it was found that Soxhlet petroleum ether extract (15.302%) had the highest anti-urease activity. Furthermore, the data obtained showed that the Soxhlet methanol extract had high efficacy in the nucleation and aggregation phase of calcium oxalate crystals. These results prove that Soxhlet methanol extract has antioxidant, anticholinesterase and anti-crystallization capabilities. Therefore, this extract can be used in the future as an antioxidant and anticholinesterase agent as well as the treatment and / or prevention of stone formation.Publication Open Access In vitro biological activities of different extracts from alcea dissecta(2022-03-01) TAŞKIN, TURGUT; DOĞAN, AHMET; TAŞKIN T., Kahvecioglu D., Turkoglu E. A., DOĞAN A., Kuzu M.Objective: Alcea genus belongs to Malvaceae family and this genus is represented by 85 taxa in the world and 21 taxa in the Flora of Turkey. The flowers of Alcea genus contain plenty of mucilage and are used for the treatment of respiratory diseases such as asthma and cough. Alcea dissecta is known as ‘Govik, Hiro, Hero’ in Turkey, and the flowers and leaves of this species have been used in the treatment of asthma, injury, colds, and gastrointestinal diseases in Turkey. To the best of our knowledge, there is no report on the effect of extraction methods on the biological activity of this plant. In addition, although this species is being used as a medical plant, there is no study of the antioxidant, antiurease, esterase, and anticholinesterase activity of the plant. Therefore, the aim of this study was to evaluate in vitro antioxidant, anti-urease, esterase, anticholinesterase activities of Alcea dissecta using a variety of extracts. Methods: The antioxidant activities of different extracts were examined by DPPH, ABTS, FRAP, and CUPRAC methods. The total phenolic compounds contained in the extracts were determined using the Folin-Ciocalteu reagent (FCR) method. Anti-urease and anticholinesterase activities of different extracts were evaluated by indophenol and Ellman methods respectively. In addition, esterase activities of plant extracts were determined. Results: In the present study, ethanol:water (1:1, v/v) and chloroform extracts obtained maceration method showed stronger DPPH and ABTS radical scavenging activity than other extracts. The chloroform extract obtained Soxhlet method was found to have higher FRAP and CUPRAC values than other extracts. It was also found that the ethanol extract obtained maceration method showed the most potent anti-urease and anticholinesterase activity. According to the results, the strongest inhibitory effect on both hCA I and II isoenzymes was shown by the petroleum ether extract obtained Soxhlet method. Conclusion: As a result, it was determined that different plant extracts have antioxidant, anti-urease, esterase, anticholinesterase activities. In addition, the data obtained from this study will shed light on future research on the biological activities of this species.Publication Open Access Bioactivities of Achillea phrygia and Bupleurum croceum based on the composition of phenolic compounds: In vitro and in silico approaches(2017-09-01) EMRE, GİZEM; DOĞAN, AHMET; Zengin G., EMRE G., Mollica A., Haznedaroglu M. Z. , DOĞAN A., Aktumsek A.This study presents the effects of the Achillea phrygia Boiss. et Bal. and Bupleurum croceum Fenzl. extracts obtained by different solvents (ethyl acetate, methanol and water) on selected enzyme inhibitory effects and antioxidant ability with screening bioactive compounds. Total and individual bioactive compounds were detected by spectrophotometric and HPLC-DAD techniques. Antioxidant abilities were evaluated by different methods including free radical scavenging (ABTS and DPPH), reducing power (CUPRAC and FRAP), phosphomolybdenum and metal chelating. Enzyme inhibitory effects were tested against cholinesterases, tyrosinase, amylase, glucosidase and lipase. Total phenolic contents were ranged from 20.52 mgGAE/g extract (B. croceum methanol extract) to 41.13 mgGAE/g extract (A. phrygia methanol extract). Generally, methanol and water extracts showed the strongest antioxidant abilities, while the ethyl acetate extracts had the most promising enzyme inhibitory effects. HPLC analysis revealed the abundance of some phenolics including rutin, quercetin, sinapic acid and chlorogenic acid, respectively. These components were also assessed using molecular modelling with the aim to study their docking properties on a set of six enzymes used in this study. Overall, these species could be suggested as valuable sources of natural-bioactive agents for developing new functional, pharmacological and health promoting ingredients. (C) 2017 Elsevier Ltd. All rights reserved.Publication Metadata only Investigation of biological activities of Paranchia kurdica subsp. kurdica var. kurdica and analysis of major phytochemicals by HPLC-DAD(2021-11-24) TAŞKIN, TURGUT; RAYAMAN, ERKAN; DOĞAN, AHMET; Ermanoğlu M., Taşkın D., Taşkın T., Rayaman E., Doğan A.