Person: ŞENKARDEŞ, İSMAİL
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ŞENKARDEŞ
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İSMAİL
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Publication Metadata only Chemical composition and bio-functional perspectives of Erica arborea L. extracts obtained by different extraction techniques: Innovative insights(ELSEVIER, 2019) DOĞAN, AHMET; Zengin, Gokhan; Cvetanovic, Aleksandra; Gasic, Uros; Stupar, Alena; Bulut, Gizem; Senkardes, Ismail; Dogan, Ahmet; Seebaluck-Sandoram, Roumita; Rengasamy, Kannan R. R.; Sinan, Kouadio Ibrahime; Mahomoodally, Mohamad FawziErica arborea L., also known as Estrella Gold, is traditionally used for several purposes. In this research, five different extraction techniques: accelerated solvent extraction (ASE), microwave-assisted extraction (MAE), maceration (MAC), soxhlet (SOE) and ultrasound-assisted extraction (UAE) were used to compare the total phenolic, flavonoids, total antioxidant activity and enzymatic activities of E. arborea extracts obtained different extraction techniques. The total phenolic and flavonoid contents were in the order of ASE > MAE > SOE > MAC > UAE. All extracts showed antioxidant, anticholinesterase, anti-tyrosinase and anti-diabetic activities. A highly sensitive method using ultra-high-pressure liquid chromatography coupled with linear ion trap-Orbitrap tandem mass spectrometry (UHPLC-LTQ-Orbitrap-MS) has been used for the qualitative analysis of obtained extracts. Seventy-two polyphenolic compounds were identified in all extracts. However, 20 components were quantified among the extracts. ASE was found to be a better extraction technique as compared to the other extraction techniques. E. arborea can be exploited in the discovery of bioactive natural products for the treatment of Alzheimer's disease, diabetes and pigmentation problems.Publication Metadata only Modern and traditional extraction techniques affect chemical composition and bioactivity of Tanacetum parthenium (L.) Sch.Bip(ELSEVIER, 2020) DOĞAN, AHMET; Zengin, Gokhan; Cvetanovic, Aleksandra; Gasic, Uros; Stupar, Alena; Bulut, Gizem; Senkardes, Ismail; Dogan, Ahmet; Sinan, Kouadio Ibrahime; Uysal, Sengul; Aumeeruddy-Elalfi, Zaahira; Aktumsek, Abdurrahman; Mahomoodally, Mohamad FawziTanacetum parthenium (L.) Sch.Bip (TP), a flowering plant, is famous in traditional medicine to prevent migraine and headache. However, there is currently a dearth of studies to advocate the phytochemical profile and biological propensities of extracts prepared from this plant. This study endeavors to highlight the biological potential of TP extracts prepared by modern (ultrasound-UAE, microwave-MAE and accelerated-ASE extractions) and traditional (maceration and Soxhlet) extraction techniques. The chemical profile of the extracts was established via ultra-high-pressure liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) technique. Sixty different polyphenolic compounds belonging to the classes of phenolic acids, flavonoid glycosides and flavonoid aglycones were recorded in the extracts. Additionally, the quantity of 17 components was measured using appropriate standards and it was found that the modern extraction gave extracts with the higher content of observed compounds than the traditional techniques. Evaluation of antioxidant activity was determined in vitro via five standard assays. The inhibitory potential of TP extracts against key enzymes implicated in the noncommunicable diseases such as diabetes (alpha-amylase and alpha-glucosidase), neurodegenerative diseases (acetyl- and butyrylcholinesterase) and skin diseases (tyrosinase), was assessed. Potent antioxidant ability of all TP extract was revealed with a predominance for the extracts yielded using the ASE method. This potent antioxidant activity of the extracts corroborated with the high phenolic (65.05 +/- 0.27 mg gallic acid equivalent (GAE)/g extract) and flavonoid contents (55A0 +/- 0.52 mg rutin equivalent (RE)/g extract). Tanacetum partheniwn extracts also showed significant alpha-glucosidase inhibitory activity (1.63-1.67 mmol acarbose equivalent (ACAE)/g extract) and moderate inhibition activity against a-amylase (0.51-0.56 mmol ACAE/g extract). The extracts also showed potent activity against cholinesterases and tyrosinase. This study tend to validate the use of TP extracts obtained by novel extraction techniques such as ASE, as potent bioactive extracts to be further studied for therapeutic bio-product development.Publication Metadata only New insights into the in vitro biological effects, in silico docking and chemical profile of clary sage - Salvia sclarea L.(ELSEVIER SCI LTD, 2018) DOĞAN, AHMET; Zengin, Gokhan; Senkardes, Ismail; Mollica, Adriano; Picot-Allain, Carene Marie Nancy; Bulut, Gizem; Dogan, Ahmet; Mahomoodally, M. FawziSalvia sclarea L. is traditionally used to manage common human ailments and is consumed as a food product. This study aimed to establish the phytochemical profile and antioxidant potential of ethyl acetate, methanol, and water extracts of Salvia sclarea. The inhibitory action of the extracts against alpha-amylase, alpha-glucosidase, acetyicholinesterase, butyrylcholinesterase, and tyrosinase was also investigated. Methanol extract showed the highest phenolic and fiavonoid contents (81.78 mg GAE/g extract and 40.59 mg RE/g extract, respectively). Reversed phase high performance liquid chromatography with diode array detector analysis revealed that S. sclarea was rich in rosmarinic acid. The water extract exhibited the lowest inhibitory activity against alpha-amylase but the upmost activity against alpha-glucosidase (0.19 and 18.24 mmol ACAE/g extract, respectively). Experimental data showed that only the water extract (8.86 mg KAE/g extract) significantly inhibited tyrosinase. Docking studies showed that quercetin binds to tyrosinase by two hydrogen and a pi-pi bonds. Salvia sclarea showed interesting biological activity against key enzymes involved in the pathogenesis of common ailments. (C) 2018 Elsevier Ltd. All rights reserved.Publication Metadata only Biologically active compounds from two members of the Asteraceae family: Tragopogon dubius Scop. and Tussilago farfara L.(TAYLOR & FRANCIS INC, 2019) DOĞAN, AHMET; Uysal, Sengul; Senkardes, Ismail; Mollica, Adriano; Zengin, Gokhan; Bulut, Gizem; Dogan, Ahmet; Glamoclija, Jasmina; Sokovic, Marina; Lobine, Devina; Mahomoodally, Fawzi M.Tragopogon dubius and Tussilago farfara are consumed as vegetables and used in folk medicine to manage common diseases. Herein, the chemical compositions and biological activities of different leaf extracts (ethyl acetate, methanol, and water) of T. dubius and T. farfara were evaluated. The antibacterial, antifungal, and antioxidant abilities of the extracts were tested using different assays including free radical scavenging, reducing power, phosphomolybdenum, and metal chelating assays. Enzyme inhibitory potentials were evaluated against cholinesterases, tyrosinase, alpha-amylase and alpha-glucosidase. Complexes of bioactive compounds (chlorogenic and rosmarinic acid) were docked into the enzymatic cavity of alpha-glucosidase and subjected to molecular dynamic calculation, enzyme conformational stability, and flexibility analysis. T. dubius and T. farfara extracts showed remarkable antioxidant potentials. Ethyl acetate extracts of T. dubius and T. farfara were the most potent inhibitors of acetylcholinesterase and butyrylcholinesterase. T. dubius ethyl acetate extract and T. farfara methanolic extract showed noteworthy activity against alpha-glucosidase. High performance liquid chromatography analysis revealed the abundance of some phenolic compounds including chlorogenic and rosmarinic acids. Ethyl acetate extract of T. dubius showed notable antifungal activity against all strains. Docking studies showed best pose for chlorogenic acid was stabilized by a network of hydrogen bonds with residues Asp1157, Asp1279, whereas rosmarinic acid showed several hydrogen bonds with Asp1157, Asp1420, Asp1526, Lys1460 and Trp1369. This study further validates the use of T. dubius and T. farfara in traditional medicine, as well as act as a stimulus for further studies for future biomedicine development. Communicated by Ramaswamy H. SarmaPublication Metadata only Influence of different extraction techniques on the chemical profile and biological properties of Anthemis cotula L.: Multifunctional aspects for potential pharmaceutical applications(ELSEVIER SCIENCE BV, 2019) DOĞAN, AHMET; Sut, Stefania; Dall'Acqua, Stefano; Zengin, Gokhan; Senkardes, Ismail; Bulut, Gizem; Cvetanovic, Aleksandra; Stupar, Alena; Mandic, Anamarija; Picot-Allain, Carene; Dogan, Ahmet; Sinan, Kouadio Ibrahime; Mahomoodally, FawziThe phytochemical composition of different extracts obtained from stinking chamomile (Anthemis cotula L) was investigated. Ethanol was used as solvent and accelerated solvent extraction (ASE), microwave assisted extraction (MAE), maceration, soxhlet extraction (SE), and ultrasound assisted extraction (UAE) were applied on plant material. Comparison of the phytochemical contents, antioxidant, and enzyme inhibitory properties were performed. The most abundant sesquiterpene in the extracts was anthecotuloide, while the most present phenolics were caffeoyl quinic acid, quercetin, and kaempferol derivatives. UAE extract was the most efficient in the extraction of sesquiterpenoids and polyphenols. Considering the assays on antioxidant activity and enzyme inhibition, ASE extract showed highest phenolic content (62.92 mg gallic acid equivalent/g extract). Likewise, this extract showed highest radical scavenging (103.44 mg trolox equivalent [TE]/g extract and 155.70 mg TE/g extract, for DPPH and ABTS assays respectively) and reducing power potential (435.32 and 317.89 mg TE/g extract, for CUPRAC and FRAP assays, respectively). The different extracts showed similar results in the enzyme inhibition assays suggesting that the extraction methods used have no effect on observed enzyme activities. Novelty of our findings are the inhibitory action of the ethanol extract of A. cotula aerial parts on key enzymes associated with Alzheimer's disease (acetyl cholinesterase, butyryl cholinesterase), type 2 diabetes (alpha-amylase, alpha-glucosidase), and skin hyperpigmentation disorders (tyrosinase). Data collected from the present work further appraises the multiple potential biological properties of stinking chamomile suggesting the need for further investigation on its constituents. (C) 2019 Elsevier B.V. All rights reserved.