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ŞENKARDEŞ, İSMAİL

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ŞENKARDEŞ

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İSMAİL

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2018 - 201912020 - 20223
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Author: TAŞKIN, TURGUT × Has files: false ×

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    Investigation on biological activities of Melissa officinalis subsp. altissima
    (2022-05-25) RAYAMAN, ERKAN; YILMAZ, BEYZA NUR; ŞENKARDEŞ, İSMAİL; TAŞKIN, TURGUT; Koç G., RAYAMAN E., ÖZDATLI KURTULUŞ Ş., Ermanoğlu M., YILMAZ B. N., ŞENKARDEŞ İ., TAŞKIN T.
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    Antioxidant, Anti-urease Activities and Genotoxic Effects of Terfezia Claveryi Methanol Extracts on Human Lymphocytes
    (MARMARA UNIV, INST HEALTH SCIENCES, 2018) ŞENKARDEŞ, İSMAİL; Taskin, Turgut; Yesil Devecioglu, Tugce; Senkardes, Ismail
    Objective: Terfezia claveryi is a dark brown color truffle and has been used extensively in human diet in Turkey. Therefore; it is an important issue to determine their biological activities and toxicity. This study aims to investigate the antioxidant and anti-urease effects of T. claveryi methanol extracts and to evaluate the genotoxicity of each extract. Methods: The antioxidant activities of methanol extracts were examined by 2,2-diphenyl-1-picryl-hydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate (ABTS) and ferric reducing/antioxidant power (FRAP) methods. The amount of total phenolic compounds contained in the extracts was determined using the Folin-Ciocalteu reagent (FCR) method. Anti-urease activities of methanol extracts were evaluated by indophenol method. In addition, potential DNA damaging effects of T. claveryi extracts on human lymphocytes were determined by the alkaline comet assay. Results: UM extract exhibited higher DPPH radical scavenging activity than MM extract [the concentration of the substance which provides the inhibition of 50% of the radical (IC50): 0.77 +/- 0.01 mg/mL, 1.17 +/- 0.02 mg/mL, respectively, p<0.0001] however, lower activity than standard (p<0.0001). Both extracts in different concentrations of T. claveryi caused DNA damage (p<0.0001) compared with solvent control DMSO. Also, total comet scores was higher in cells which had been incubated with MM extract (p<0.05). UM extract exhibited higher ferric reducing and anti-urease activity than MM extract but not found statistically different; also activities of both extract were found lower than positive controls of each methods (p<0.0001). Conclusion: T. claveryi methanol extracts induced genotoxicity on lymphocytes and showed very low antioxidant activity compared to standards. This preliminary study should be confirmed with further studies and in different cell lines.
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    Phytochemical screening and evaluation of antioxidant, enzyme inhibition, anti-proliferative and calcium oxalate anti-crystallization activities of Micromeria fruticosa spp. brachycalyx and Rhus coriaria
    (ELSEVIER, 2020) ŞENKARDEŞ, İSMAİL; Taskin, Turgut; Dogan, Murat; Yilmaz, Beyza Nur; Senkardes, Ismail
    Micromeria fruticosa spp. brachycalyx species (Lamiaceae) is used as a spice and flavoring agent. Rhus coriaria (Sumac) is widely used both spice and medicinally. In this study, antioxidant, anti-urease, anticholinesterase, anti-proliferative and calcium oxalate anti-crystallization activities of different extracts from M. fruticosa spp. brachycalyx aerial parts and R. coriaria leaves were investigated. Besides, the phytochemical content of these species was analyzed qualitatively and quantitatively. R. coriaria methanol extract showed stronger antioxidant (DPPH, FRAP, CUPRAC, ARTS) and anticholinesterase (46.85%) activity than M. fruticosa spp. brachycalyx extracts. M. fruticosa spp. brachycalyx methanol extract was found to have the highest anti-urease (11.39%) and anti-proliferative (MCF-7: 53.51%) potential. It was observed that the methanol extracts of both plants had a significant inhibition effect on calcium oxalate crystallization. R. coriaria methanol extract was determined to contain high amounts of total flavonoids (0.177 mgQE/mg extract) and tannins (0.365 mg TAE/mg extract) contents. The extracts from both plants have no cytotoxic effects on the healthy cell (L-929) and have strong biological activities in parallel with traditional use, suggesting that these plants may be used as natural sources in the future.
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    Investigation of the Biological Activities of Different Extracts from Dipsacus laciniatus Aerial
    (BENTHAM SCIENCE PUBL, 2020) ŞENKARDEŞ, İSMAİL; Taskin, Turgut; Akkilic, Yeliz; Celik, Berna Ozbek; Senkardes, Ismail; Ozakpinar, Ozlem B.
    Background: The aerial parts of Dipsacus laciniatus are traditionally used in the treatment of colds as cold tea; decoction prepared from its roots and aerial parts are used for the treatment of diabetes and cancer, respectively. The extraction procedures and solvents are important steps in the processing of bioactive constituents from the plant materials. Objective: The aim of this study was to evaluate in vitro antioxidant, antimicrobial, anticholinesterase and cytotoxic activities for the first time in plant's different extracts. Methods: The antioxidant activities of extracts were examined using FRAP and CUPRAC methods, including total phenolic and tlavonoids contents. In addition, the antimicrobial, anticholinesterase and cytotoxic activities of extracts were investigated using mnicrodilution, Ellman and MTT methods, respectively. Results: In the present study, methanol extract showed stronger iron (III) ion reduction (FRAP) and copper (II) ion reducing antioxidant capacity (('UPRAC) than other extracts and also contained higher total phenolic and flavonoid compounds. It was also found that there was a linear relationship between antioxidant activity and phenolic compounds in this study. It was demonstrated that the chloroform and methanol extracts showed moderate antimicrobial activity against S. epidermidis and C. albicatis strains. The chloroform extract had a strong and selective cytotoxic activity for the MCF-7 and HT-29 cell lines, while the methanol extract showed low cytotoxic activity on the same cell lines. In addition, according to the obtained data, chloroform extract showed a toxic effect on normal cell line (NIH/3T3), but other extracts showed no toxic effect. Conclusion: Since methanol extract has no toxic effect on normal cell line and has strong antioxidant, anticholinesterase and antimicrobial activity, it is thought that this extract may be useful as an antioxidant, anticholinesterase and antimicrobial agents in the future.