Person: ŞENKARDEŞ, İSMAİL
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ŞENKARDEŞ
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İSMAİL
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Publication Metadata only Chemical composition and bio-functional perspectives of Erica arborea L. extracts obtained by different extraction techniques: Innovative insights(ELSEVIER, 2019) DOĞAN, AHMET; Zengin, Gokhan; Cvetanovic, Aleksandra; Gasic, Uros; Stupar, Alena; Bulut, Gizem; Senkardes, Ismail; Dogan, Ahmet; Seebaluck-Sandoram, Roumita; Rengasamy, Kannan R. R.; Sinan, Kouadio Ibrahime; Mahomoodally, Mohamad FawziErica arborea L., also known as Estrella Gold, is traditionally used for several purposes. In this research, five different extraction techniques: accelerated solvent extraction (ASE), microwave-assisted extraction (MAE), maceration (MAC), soxhlet (SOE) and ultrasound-assisted extraction (UAE) were used to compare the total phenolic, flavonoids, total antioxidant activity and enzymatic activities of E. arborea extracts obtained different extraction techniques. The total phenolic and flavonoid contents were in the order of ASE > MAE > SOE > MAC > UAE. All extracts showed antioxidant, anticholinesterase, anti-tyrosinase and anti-diabetic activities. A highly sensitive method using ultra-high-pressure liquid chromatography coupled with linear ion trap-Orbitrap tandem mass spectrometry (UHPLC-LTQ-Orbitrap-MS) has been used for the qualitative analysis of obtained extracts. Seventy-two polyphenolic compounds were identified in all extracts. However, 20 components were quantified among the extracts. ASE was found to be a better extraction technique as compared to the other extraction techniques. E. arborea can be exploited in the discovery of bioactive natural products for the treatment of Alzheimer's disease, diabetes and pigmentation problems.Publication Metadata only Antioxidant abilities, key enzyme inhibitory potential and phytochemical profile of Tanacetum poteriifolium Grierson(ELSEVIER, 2019) ŞENKARDEŞ, İSMAİL; Zengin, Gokhan; Sieniawska, Elwira; Senkardes, Ismail; Picot-Allain, Marie Carene Nancy; Sinan, Kouadio Ibrahime; Mahomoodally, Mohamad FawziThe members of the genus Tanacetum have a long history in traditional medicine to manage several diseases. Thus, the present study attempts to determine the chemical composition and to probe into the biological activities of Tanacetum poteriifolium Grierson, a poorly studied medicinal plant. The enzyme inhibitory and antioxidant properties of the ethyl acetate, methanol, and water extracts were assessed using standard in vitro assays. Biological data were further investigated by multivariate analysis tools. The water extract showed the highest concentration of phenolics (83.38 mg gallic acid equivalent (GAE)/g extract), and was a potent radical scavenger (238.12 and 282.54 mg Trolox equivalent (TE)/g extract, towards 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS), respectively) and had potent reducing power (555.03 and 285.79 mg TE/g extract, towards cupric reducing antioxidant capacity (CUPRAC) and ferric reducing antioxidant power (FRAP), respectively). The ethyl acetate extract (41.07 mg ethylenediaminetetraacetic acid equivalent (EDTAE)/g extract), rich in flavonoids (43.55 mg rutin equivalent (RE)/g extract), was a potent metal chelator. Besides, the ethyl acetate extract exhibited strong inhibitory action on cholinesterases (3.47 and 3.46 mg galantamine equivalent (GALAE)/g extract) and alpha-glucosidase (23.67 mmol acarbose equivalent (ACAE)/g extract). Tyrosinase was actively inhibited in the presence of the methanol extract of T. poteriifolium (128.54 mg kojic acid equivalent (KAE)/g extract). Liquid chromatography-electrospray ionisation quadrupole time-of-flight mass spectrometry analysis revealed the presence of quinic acid and methylquercetin derivatives in T. poteriifolium ethyl acetate extract. Two unknown compounds having m/z values 289.17, 267.23, 235.13, 211.14, 185.12, 121.06 and 275.21, 211.15, 171.11, 121.11 were identified from the T. poteriifolium methanol extract. Principal component analysis was performed to obtain an overview of the influence of solvents on T. poteriifolium biological activities which were satisfactorily discriminated. This study provides valuable baseline data on the biological activity of T. poteriifolium towards important enzymes targeted in the management of global health problems, such as Alzheimer's disease, diabetes, and epidermal hyperpigmentation problems.Publication Metadata only Anti-quorum sensing and anti-biofilm activities of Hypericum perforatum extracts against Pseudomonas aeruginosa(ELSEVIER IRELAND LTD, 2019) DOĞAN, AHMET; Dogan, Sule; Gokalsin, Baris; Senkardes, Ismail; Dogan, Ahmet; Sesal, N. CenkEthnopharmacological relevance: Hypericum perforatum L. (Hypericaceae) has been used as a traditional therapeutic for skin wounds, burns, cuts and stomach ailments including stomach ache, ulcers for a long time in many societies. Although many studies about its antibacterial properties can be found, there is a lack of studies about its quorum sensing inhibition properties, which effects bacterial vulnerability directly, on Pseudomonas aeruginosa. Aim of the study: Evaluation of anti-quorum sensing (anti-QS) and anti-biofilm activity of ethanol, methanol, acetone and ultra-sonicated extracts of Hypericum perforatum L. (HP) which is a well-known wound healer, against P. aeruginosa. Materials and methods: Aerial parts of HP were extracted with ethanol, methanol and acetone. In addition, separate extractions with ultrasonication were carried out with same solvents. Anti-QS activity tests with different doses of HP extracts were performed by employing biomonitor strains, of which the promoter of QS regulating and green fluorescent protein (GFP) genes were fusioned. For anti-biofilm activity, HP extracts were applied to wild type PAO1 strains and biofilm inhibition was quantified via crystal violet staining method. Results: HP's ethanol, methanol and acetone extracts (250 mu g/ml doses) inhibited LasIR signalling pathway up to 65.43%, 59.60%, 55.95% and same solvent extracts obtained with ultrasonication inhibited 71.33%, 64.47%, 57.35% respectively. Moreover, inhibition rates of Rh1IR pathway were 28.80%, 50.83%, 45.84% for ethanol, methanol, acetone extracts (250 mu g/ml doses) and 51.43%, 57.41%, 50.02% for ultrasonication extracts (250 mu g/ml doses), compared to untreated controls. In the experiments, ethanol, methanol, acetone and ultra-sonicated extracts of HP did not inhibit biofilm formation. Conclusions: This study shows that HP plant is capable for blocking of las and rhl QS systems of P. aeruginosa. However, it was observed that ethanol, methanol and acetone extract of the plant samples did not show anti-biofilm activity against P. aeruginosa. This led us to thinking that biofilm formation was caused via another pathway such as IQS or PQS. Further studies with isolated active compounds of HP might give a better understanding of the effects on biofilm formation of P. aeruginosa.Publication Metadata only Tanacetum vulgare L. (Tansy) as an effective bioresource with promising pharmacological effects from natural arsenal(PERGAMON-ELSEVIER SCIENCE LTD, 2021) ŞENKARDEŞ, İSMAİL; Ak, Gunes; Gevrenova, Reneta; Sinan, Kouadio Ibrahime; Zengin, Gokhan; Zheleva, Dimitrina; Mahomoodally, Mohamad Fawzi; Senkardes, Ismail; Brunetti, Luigi; Leone, Sheila; Di Simone, Simonetta Cristina; Recinella, Lucia; Chiavaroli, Annalisa; Menghini, Luigi; Orlando, Giustino; Ferrante, ClaudioThe Tanacetum genus is a big treasure with the presence of biologically-active compounds and members of this genus are widely used for the treatment of several diseases in traditional medicine system. Considering this fact, we aimed to analyze the extracts from Tanacetum vulgare L. in case of chemical profiles and biological effects. Chemical characterization was performed by using UHPLC-HRMS technique and showed the presence of several phytochemical groups (107 compounds were identified, including phenolic acids, flavonoids, terpenoids and fatty acids. Biological abilities were examined by using antioxidant (DPPH, ABTS, FRAP, CUPRAC, metal chelating and phosphomolybdenum assays) and enzyme inhibition (tyrosinase, amylase, glucosidase and cholinesterase) properties. Pharmaco-toxicological investigations were also performed with the aim to identify limits of biocompatibility, anti-oxidant and neuromodulatory effects, in hypothalamic HypoE22 cells. A bio-informatic analysis was also carried to unravel the putative protein-targets for the observed biological effects. Generally, the tested hexane and hydroalcoholic extracts displayed stronger activities in antioxidant and enzyme inhibitory assays, when compared with water. In addition, multivariate analysis was performed to understand the differences in both solvents and plant parts and we clearly observed the separation of these parameters. The extracts (10 mu g/mL) also stimulated DAT and inhibited TNF alpha and BDNF gene expression, in HypoE22 cells. In parallel, the extracts were also able to stimulate norepinephrine release from this cell line. By contrast, in the concentration range 50-100 mu g/mL, the extracts reduced the HypoE22 viability, thus demonstrating cytotoxicity at concentrations 5-10 fold higher compared to those effective as neuromodulatory. Our observations manifested that T. vulgare has several beneficial effects and it can be used as a potential natural raw material for designing further health-promoting applications in nutraceutical, cosmeceutical, and pharmaceutical areas.Publication Metadata only Modern and traditional extraction techniques affect chemical composition and bioactivity of Tanacetum parthenium (L.) Sch.Bip(ELSEVIER, 2020) DOĞAN, AHMET; Zengin, Gokhan; Cvetanovic, Aleksandra; Gasic, Uros; Stupar, Alena; Bulut, Gizem; Senkardes, Ismail; Dogan, Ahmet; Sinan, Kouadio Ibrahime; Uysal, Sengul; Aumeeruddy-Elalfi, Zaahira; Aktumsek, Abdurrahman; Mahomoodally, Mohamad FawziTanacetum parthenium (L.) Sch.Bip (TP), a flowering plant, is famous in traditional medicine to prevent migraine and headache. However, there is currently a dearth of studies to advocate the phytochemical profile and biological propensities of extracts prepared from this plant. This study endeavors to highlight the biological potential of TP extracts prepared by modern (ultrasound-UAE, microwave-MAE and accelerated-ASE extractions) and traditional (maceration and Soxhlet) extraction techniques. The chemical profile of the extracts was established via ultra-high-pressure liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) technique. Sixty different polyphenolic compounds belonging to the classes of phenolic acids, flavonoid glycosides and flavonoid aglycones were recorded in the extracts. Additionally, the quantity of 17 components was measured using appropriate standards and it was found that the modern extraction gave extracts with the higher content of observed compounds than the traditional techniques. Evaluation of antioxidant activity was determined in vitro via five standard assays. The inhibitory potential of TP extracts against key enzymes implicated in the noncommunicable diseases such as diabetes (alpha-amylase and alpha-glucosidase), neurodegenerative diseases (acetyl- and butyrylcholinesterase) and skin diseases (tyrosinase), was assessed. Potent antioxidant ability of all TP extract was revealed with a predominance for the extracts yielded using the ASE method. This potent antioxidant activity of the extracts corroborated with the high phenolic (65.05 +/- 0.27 mg gallic acid equivalent (GAE)/g extract) and flavonoid contents (55A0 +/- 0.52 mg rutin equivalent (RE)/g extract). Tanacetum partheniwn extracts also showed significant alpha-glucosidase inhibitory activity (1.63-1.67 mmol acarbose equivalent (ACAE)/g extract) and moderate inhibition activity against a-amylase (0.51-0.56 mmol ACAE/g extract). The extracts also showed potent activity against cholinesterases and tyrosinase. This study tend to validate the use of TP extracts obtained by novel extraction techniques such as ASE, as potent bioactive extracts to be further studied for therapeutic bio-product development.