Person: ŞENKARDEŞ, İSMAİL
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ŞENKARDEŞ
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İSMAİL
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Publication Metadata only Chemical composition and bio-functional perspectives of Erica arborea L. extracts obtained by different extraction techniques: Innovative insights(ELSEVIER, 2019) DOĞAN, AHMET; Zengin, Gokhan; Cvetanovic, Aleksandra; Gasic, Uros; Stupar, Alena; Bulut, Gizem; Senkardes, Ismail; Dogan, Ahmet; Seebaluck-Sandoram, Roumita; Rengasamy, Kannan R. R.; Sinan, Kouadio Ibrahime; Mahomoodally, Mohamad FawziErica arborea L., also known as Estrella Gold, is traditionally used for several purposes. In this research, five different extraction techniques: accelerated solvent extraction (ASE), microwave-assisted extraction (MAE), maceration (MAC), soxhlet (SOE) and ultrasound-assisted extraction (UAE) were used to compare the total phenolic, flavonoids, total antioxidant activity and enzymatic activities of E. arborea extracts obtained different extraction techniques. The total phenolic and flavonoid contents were in the order of ASE > MAE > SOE > MAC > UAE. All extracts showed antioxidant, anticholinesterase, anti-tyrosinase and anti-diabetic activities. A highly sensitive method using ultra-high-pressure liquid chromatography coupled with linear ion trap-Orbitrap tandem mass spectrometry (UHPLC-LTQ-Orbitrap-MS) has been used for the qualitative analysis of obtained extracts. Seventy-two polyphenolic compounds were identified in all extracts. However, 20 components were quantified among the extracts. ASE was found to be a better extraction technique as compared to the other extraction techniques. E. arborea can be exploited in the discovery of bioactive natural products for the treatment of Alzheimer's disease, diabetes and pigmentation problems.Publication Open Access Chemical Characterization and Bioactive Properties of Different Extracts from Fibigia clypeata, an Unexplored Plant Food(MDPI, 2020-06-01) ŞENKARDEŞ, İSMAİL; Zengin, Gokhan; Mahomoodally, Mohamad Fawzi; Rocchetti, Gabriele; Lucini, Luigi; Sieniawska, Elwira; Swiatek, Lukasz; Rajtar, Barbara; Polz-Dacewicz, Malgorzata; Senkardes, Ismail; Aktumsek, Abdurrahman; Picot-Allain, Marie Carene Nancy; Montesano, DomenicoFibigia clypeata(L.) Medik. is a poorly studied plant species belonging to the Brassicaceae family, and usually used as cress in the salads. The current investigation aimed at assessing the antioxidant potential and inhibitory activity of ethyl acetate, methanol, and aqueous extracts ofF. clypeataagainst key enzymes targeted in the management of type II diabetes (alpha-amylase and alpha-glucosidase), Alzheimer's disease (acetylcholinesterase and butyrylcholinesterase), and skin hyperpigmentation (tyrosinase). Cytotoxicity of the extracts was also determined using normal VERO and cancer FaDu and SCC-25 cell lines. Besides, LC-MS was employed to investigate the detailed phytochemical profiles of the extracts. The methanol extract showed potent enzyme inhibitory activity (4.87 mg galantamine equivalent/g, 3.52 mg galantamine equivalent/g, 126.80 mg kojic acid equivalent/g, and 24.68 mg acarbose equivalent/g, for acetylcholinesterase, butyrylcholinesterase, tyrosinase, and alpha-glucosidase, respectively) and antioxidant potential (96.52, 109.10, 154.02, and 104.85 mg trolox equivalent/g, for DPPH, ABTS, CUPRAC, and FRAP assays, respectively). Interestingly, caffeic acid-O-hexoside derivative, caffeyl alcoholO-glucopyranoside, and ferulic acid derivative were identified in all extracts.F. clypeataextracts showed no cytotoxicity towards VERO cell line and a weak cytotoxic potential against FaDu and SCC-25 cell lines. Interesting scientific evidence gathered from the present study support further investigation onF. clypeatain the view of designing and developing a novel therapeutic agent for the management of Alzheimer's disease, type II diabetes, skin hyperpigmentation problems, as well as cancer.Publication Open Access The in vitro and in vivo investigation of biological activities and phenolic analysis of Helichrysum plicatum subsp. plicatum(UNIV SAO PAULO, CONJUNTO QUIMICAS, 2020) ŞENKARDEŞ, İSMAİL; Taskin, Turgut; Gezmis, Tugba; Cam, Muhammet Emin; Taskin, Duygu; Celik, Berna Ozbek; Senkardes, Ismail; Suzgec-Selcuk, SevdaIn Turkey, Helichrysum genus is represented by 26 taxa belonging to 20 species in Turkish flora of which 14 ones arc endemic to Turkey. The aerial parts of Helichrysum plicatum subsp. plicatum are used kidney stones, kidney and stomach ailments. The extraction procedures and solvents are important step in processing of bioactive constituents from the plant materials. Therefore, the aim of this study was to evaluate in vitro antioxidant, antimicrobial, anti-urease, anticholinesterase and in vivo anti-inflammatory activities of Helichrysum plicatum subsp. plicatum different extracts. In addition, the phenolic characterization of the Soxhlet and maceration methanol extracts which showed significant antioxidant, anti-urease, antimicrobial, anti-inflammatmy and anticholinesterase activities were performed by HPLC-DAD and LC-MS/MS. In the present study, the Soxhlet methanol extract exhibited strong antioxidant, antimicrobial and anticholinestemse activities than other extracts. The maceration methanol extract showed the strongest anti-urease activity. The Soxhlet methanol and maceration methanol extracts showed in vivo anti-inflammatory activities very close to each other. As a result of this study, chlorogenic acid, dicaffeoylquinic acid, luteolin, luteolin-7-O-glucoside, naringenin-O-hexoside and isoquercitrin compounds were analysed in plant. Therefore, it is thought that methanol extracts can be used as a natural source in the future for food and pharmaceutical industries.Publication Metadata only Antioxidant abilities, key enzyme inhibitory potential and phytochemical profile of Tanacetum poteriifolium Grierson(ELSEVIER, 2019) ŞENKARDEŞ, İSMAİL; Zengin, Gokhan; Sieniawska, Elwira; Senkardes, Ismail; Picot-Allain, Marie Carene Nancy; Sinan, Kouadio Ibrahime; Mahomoodally, Mohamad FawziThe members of the genus Tanacetum have a long history in traditional medicine to manage several diseases. Thus, the present study attempts to determine the chemical composition and to probe into the biological activities of Tanacetum poteriifolium Grierson, a poorly studied medicinal plant. The enzyme inhibitory and antioxidant properties of the ethyl acetate, methanol, and water extracts were assessed using standard in vitro assays. Biological data were further investigated by multivariate analysis tools. The water extract showed the highest concentration of phenolics (83.38 mg gallic acid equivalent (GAE)/g extract), and was a potent radical scavenger (238.12 and 282.54 mg Trolox equivalent (TE)/g extract, towards 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS), respectively) and had potent reducing power (555.03 and 285.79 mg TE/g extract, towards cupric reducing antioxidant capacity (CUPRAC) and ferric reducing antioxidant power (FRAP), respectively). The ethyl acetate extract (41.07 mg ethylenediaminetetraacetic acid equivalent (EDTAE)/g extract), rich in flavonoids (43.55 mg rutin equivalent (RE)/g extract), was a potent metal chelator. Besides, the ethyl acetate extract exhibited strong inhibitory action on cholinesterases (3.47 and 3.46 mg galantamine equivalent (GALAE)/g extract) and alpha-glucosidase (23.67 mmol acarbose equivalent (ACAE)/g extract). Tyrosinase was actively inhibited in the presence of the methanol extract of T. poteriifolium (128.54 mg kojic acid equivalent (KAE)/g extract). Liquid chromatography-electrospray ionisation quadrupole time-of-flight mass spectrometry analysis revealed the presence of quinic acid and methylquercetin derivatives in T. poteriifolium ethyl acetate extract. Two unknown compounds having m/z values 289.17, 267.23, 235.13, 211.14, 185.12, 121.06 and 275.21, 211.15, 171.11, 121.11 were identified from the T. poteriifolium methanol extract. Principal component analysis was performed to obtain an overview of the influence of solvents on T. poteriifolium biological activities which were satisfactorily discriminated. This study provides valuable baseline data on the biological activity of T. poteriifolium towards important enzymes targeted in the management of global health problems, such as Alzheimer's disease, diabetes, and epidermal hyperpigmentation problems.Publication Metadata only Anti-quorum sensing and anti-biofilm activities of Hypericum perforatum extracts against Pseudomonas aeruginosa(ELSEVIER IRELAND LTD, 2019) DOĞAN, AHMET; Dogan, Sule; Gokalsin, Baris; Senkardes, Ismail; Dogan, Ahmet; Sesal, N. CenkEthnopharmacological relevance: Hypericum perforatum L. (Hypericaceae) has been used as a traditional therapeutic for skin wounds, burns, cuts and stomach ailments including stomach ache, ulcers for a long time in many societies. Although many studies about its antibacterial properties can be found, there is a lack of studies about its quorum sensing inhibition properties, which effects bacterial vulnerability directly, on Pseudomonas aeruginosa. Aim of the study: Evaluation of anti-quorum sensing (anti-QS) and anti-biofilm activity of ethanol, methanol, acetone and ultra-sonicated extracts of Hypericum perforatum L. (HP) which is a well-known wound healer, against P. aeruginosa. Materials and methods: Aerial parts of HP were extracted with ethanol, methanol and acetone. In addition, separate extractions with ultrasonication were carried out with same solvents. Anti-QS activity tests with different doses of HP extracts were performed by employing biomonitor strains, of which the promoter of QS regulating and green fluorescent protein (GFP) genes were fusioned. For anti-biofilm activity, HP extracts were applied to wild type PAO1 strains and biofilm inhibition was quantified via crystal violet staining method. Results: HP's ethanol, methanol and acetone extracts (250 mu g/ml doses) inhibited LasIR signalling pathway up to 65.43%, 59.60%, 55.95% and same solvent extracts obtained with ultrasonication inhibited 71.33%, 64.47%, 57.35% respectively. Moreover, inhibition rates of Rh1IR pathway were 28.80%, 50.83%, 45.84% for ethanol, methanol, acetone extracts (250 mu g/ml doses) and 51.43%, 57.41%, 50.02% for ultrasonication extracts (250 mu g/ml doses), compared to untreated controls. In the experiments, ethanol, methanol, acetone and ultra-sonicated extracts of HP did not inhibit biofilm formation. Conclusions: This study shows that HP plant is capable for blocking of las and rhl QS systems of P. aeruginosa. However, it was observed that ethanol, methanol and acetone extract of the plant samples did not show anti-biofilm activity against P. aeruginosa. This led us to thinking that biofilm formation was caused via another pathway such as IQS or PQS. Further studies with isolated active compounds of HP might give a better understanding of the effects on biofilm formation of P. aeruginosa.Publication Metadata only Tanacetum vulgare L. (Tansy) as an effective bioresource with promising pharmacological effects from natural arsenal(PERGAMON-ELSEVIER SCIENCE LTD, 2021) ŞENKARDEŞ, İSMAİL; Ak, Gunes; Gevrenova, Reneta; Sinan, Kouadio Ibrahime; Zengin, Gokhan; Zheleva, Dimitrina; Mahomoodally, Mohamad Fawzi; Senkardes, Ismail; Brunetti, Luigi; Leone, Sheila; Di Simone, Simonetta Cristina; Recinella, Lucia; Chiavaroli, Annalisa; Menghini, Luigi; Orlando, Giustino; Ferrante, ClaudioThe Tanacetum genus is a big treasure with the presence of biologically-active compounds and members of this genus are widely used for the treatment of several diseases in traditional medicine system. Considering this fact, we aimed to analyze the extracts from Tanacetum vulgare L. in case of chemical profiles and biological effects. Chemical characterization was performed by using UHPLC-HRMS technique and showed the presence of several phytochemical groups (107 compounds were identified, including phenolic acids, flavonoids, terpenoids and fatty acids. Biological abilities were examined by using antioxidant (DPPH, ABTS, FRAP, CUPRAC, metal chelating and phosphomolybdenum assays) and enzyme inhibition (tyrosinase, amylase, glucosidase and cholinesterase) properties. Pharmaco-toxicological investigations were also performed with the aim to identify limits of biocompatibility, anti-oxidant and neuromodulatory effects, in hypothalamic HypoE22 cells. A bio-informatic analysis was also carried to unravel the putative protein-targets for the observed biological effects. Generally, the tested hexane and hydroalcoholic extracts displayed stronger activities in antioxidant and enzyme inhibitory assays, when compared with water. In addition, multivariate analysis was performed to understand the differences in both solvents and plant parts and we clearly observed the separation of these parameters. The extracts (10 mu g/mL) also stimulated DAT and inhibited TNF alpha and BDNF gene expression, in HypoE22 cells. In parallel, the extracts were also able to stimulate norepinephrine release from this cell line. By contrast, in the concentration range 50-100 mu g/mL, the extracts reduced the HypoE22 viability, thus demonstrating cytotoxicity at concentrations 5-10 fold higher compared to those effective as neuromodulatory. Our observations manifested that T. vulgare has several beneficial effects and it can be used as a potential natural raw material for designing further health-promoting applications in nutraceutical, cosmeceutical, and pharmaceutical areas.Publication Metadata only Modern and traditional extraction techniques affect chemical composition and bioactivity of Tanacetum parthenium (L.) Sch.Bip(ELSEVIER, 2020) DOĞAN, AHMET; Zengin, Gokhan; Cvetanovic, Aleksandra; Gasic, Uros; Stupar, Alena; Bulut, Gizem; Senkardes, Ismail; Dogan, Ahmet; Sinan, Kouadio Ibrahime; Uysal, Sengul; Aumeeruddy-Elalfi, Zaahira; Aktumsek, Abdurrahman; Mahomoodally, Mohamad FawziTanacetum parthenium (L.) Sch.Bip (TP), a flowering plant, is famous in traditional medicine to prevent migraine and headache. However, there is currently a dearth of studies to advocate the phytochemical profile and biological propensities of extracts prepared from this plant. This study endeavors to highlight the biological potential of TP extracts prepared by modern (ultrasound-UAE, microwave-MAE and accelerated-ASE extractions) and traditional (maceration and Soxhlet) extraction techniques. The chemical profile of the extracts was established via ultra-high-pressure liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) technique. Sixty different polyphenolic compounds belonging to the classes of phenolic acids, flavonoid glycosides and flavonoid aglycones were recorded in the extracts. Additionally, the quantity of 17 components was measured using appropriate standards and it was found that the modern extraction gave extracts with the higher content of observed compounds than the traditional techniques. Evaluation of antioxidant activity was determined in vitro via five standard assays. The inhibitory potential of TP extracts against key enzymes implicated in the noncommunicable diseases such as diabetes (alpha-amylase and alpha-glucosidase), neurodegenerative diseases (acetyl- and butyrylcholinesterase) and skin diseases (tyrosinase), was assessed. Potent antioxidant ability of all TP extract was revealed with a predominance for the extracts yielded using the ASE method. This potent antioxidant activity of the extracts corroborated with the high phenolic (65.05 +/- 0.27 mg gallic acid equivalent (GAE)/g extract) and flavonoid contents (55A0 +/- 0.52 mg rutin equivalent (RE)/g extract). Tanacetum partheniwn extracts also showed significant alpha-glucosidase inhibitory activity (1.63-1.67 mmol acarbose equivalent (ACAE)/g extract) and moderate inhibition activity against a-amylase (0.51-0.56 mmol ACAE/g extract). The extracts also showed potent activity against cholinesterases and tyrosinase. This study tend to validate the use of TP extracts obtained by novel extraction techniques such as ASE, as potent bioactive extracts to be further studied for therapeutic bio-product development.Publication Open Access Qualitative Fingerprint Analysis and Multidirectional Assessment of Different Crude Extracts and Essential Oil from Wild Artemisia santonicum L.(MDPI, 2019-08-07) ŞENKARDEŞ, İSMAİL; Ferrante, Claudio; Zengin, Gokhan; Menghini, Luigi; Diuzheva, Alina; Jeko, Jozsef; Cziaky, Zoltan; Recinella, Lucia; Chiavaroli, Annalisa; Leone, Sheila; Brunetti, Luigi; Lobine, Devina; Senkardes, Ismail; Mahomoodally, Mohamad Fawzi; Orlando, GiustinoArtemisia species are used as folk medicines in several countries. This work was aimed to shed more light on the effect of methanol, water, ethyl acetate extracts, and essential oil (EO) of A. santonicum on selected enzymes (cholinesterase, tyrosinase alpha-amylase, and alpha-glucosidase) as well of their antioxidant and pharmacological effects. The chemical profile of the essential oil was determined using gas chromatography coupled to mass spectrometry (GC-MS) analysis, while the extracts were chemically characterized by high performance liquid chromatography coupled to mass spectrometry (HPLC-MS). Forty-nine constituents were identified and camphor (36.6%), 1,8-cineole (10.2%), alpha-thujone (10.1%), borneol (4.5%), and beta-thujone (3.6%) were the major components. Overall, 45, 74, and 67 components were identified from the ethyl acetate, methanol, and water extracts, respectively. The EO and extracts showed significant antioxidant properties, in a cell-free model; particularly, methanol and water extracts revealed promising sources of antioxidant compounds. Additionally, we evaluated protective effects of EO and extracts in isolated rat colon tissue challenged with lipopolysaccharide (LPS), as an ex vivo model of colon inflammation, and human colon cancer HCT116 cell line. Particularly, we observed that, among all tested samples, A. santonicum ethyl acetate displayed the best pharmacological profile, being able to blunt LPS-induced levels of all tested biomarkers of inflammation and oxidative stress, including colon nitrites, lactate dehydrogenase, prostaglandin E-2, and serotonin. Additionally, this extract was also able to reduce HCT116 cell viability, thus suggesting potential antiproliferative effects against colon cancer cells. Based on our results, A. santonicum has great potential for developing novel functional agents including pharmaceuticals, cosmeceuticals, and nutraceuticals.