Person: TURANLI, BESTE
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TURANLI
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BESTE
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Publication Open Access Comparative analysis of diisononyl phthalate and di(isononyl)cyclohexane-1,2 dicarboxylate plasticizers in regulation of lipid metabolism in 3T3-L1 cells(2023-01-01) BEREKETOĞLU, CEYHUN; TURANLI, BESTE; BEREKETOĞLU C., Häggblom I., TURANLI B., Pradhan A.Diisononyl phthalate (DINP) and di(isononyl)cyclohexane-1,2-dicarboxylate (DINCH) are plasticizers introduced to replace previously used phthalate plasticizers in polymeric products. Exposure to DINP and DINCH has been shown to impact lipid metabolism. However, there are limited studies that address the mechanisms of toxicity of these two plasticizers. Here, a comparative toxicity analysis has been performed to evaluate the impacts of DINP and DINCH on 3T3-L1 cells. The preadipocyte 3T3-L1 cells were exposed to 1, 10, and 100 μM of DINP or DINCH for 10 days and assessed for lipid accumulation, gene expression, and protein analysis. Lipid staining showed that higher concentrations of DINP and DINCH can induce adipogenesis. The gene expression analysis demonstrated that both DINP and DINCH could alter the expression of lipid-related genes involved in adipogenesis. DINP and DINCH upregulated Pparγ, Pparα, C/EBPα Fabp4, and Fabp5, while both compounds significantly downregulated Fasn and Gata2. Protein analysis showed that both DINP and DINCH repressed the expression of FASN. Additionally, we analyzed an independent transcriptome dataset encompassing temporal data on lipid differentiation within 3T3-L1 cells. Subsequently, we derived a gene set that accurately portrays significant pathways involved in lipid differentiation, which we subsequently subjected to experimental validation through quantitative polymerase chain reaction. In addition, we extended our analysis to encompass a thorough assessment of the expression profiles of this identical gene set across 40 discrete transcriptome datasets that have linked to diverse pathological conditions to foreseen any potential association with DINP and DINCH exposure. Comparative analysis indicated that DINP could be more effective in regulating lipid metabolism.Publication Metadata only Systems biomarkers in psoriasis: Integrative evaluation of computational and experimental data at transcript and protein levels(ELSEVIER SCIENCE BV, 2018) TURANLI, BESTE; Sevimoglu, Tuba; Turanli, Beste; Bereketoglu, Ceyhun; Arga, Kazim Yalcin; Karadag, Ayse SerapPsoriasis is a complex autoimmune disease with multiple genes and proteins being involved in its pathogenesis. Despite the efforts performed to understand mechanisms of psoriasis pathogenesis and to identify diagnostic and prognostic targets, disease-specific and effective biomarkers were still not available. This study is compiled regarding clinical validation of computationally proposed biomarkers at gene and protein expression levels through qRT-PCR and ELISA techniques using skin biopsies and blood plasma. We identified several gene and protein clusters as systems biomarkers and presented the importance of gender difference in psoriasis. A gene cluster comprising of P13, IRF9, IFIT1 and NMI were found as positively correlated and differentially co-expressed for women, whereas SUB1 gene was also included in this cluster for men. The differential expressions of IRF9 and NMI in women and SUB1 in men were validated at gene expression level via qRT-PCR. At protein level, PI3 was abundance in disease states of both genders, whereas PC4 protein and WIF1 protein were significantly higher in healthy states than disease states of male group and female group, respectively. Regarding abundancy of PI3 and WIF1 proteins in women, and PI3 and PC4 in men may be assumed as systems biomarkers at protein level.Publication Metadata only Simultaneous nitrate and perchlorate removal from groundwater by heterotrophic-autotrophic sequential system(ELSEVIER SCI LTD, 2017) TURANLI, BESTE; Ucar, Deniz; Cokgor, Emine Ubay; Sahinkaya, Erkan; Cetin, Umut; Bereketoglu, Ceyhun; Calimlioglu, Beste; Goncu, Betul; Yurtsever, AdemPerchlorate is a naturally occurring and manufactured chemical anion and can be present in water sources together with nitrate. This study aims at (1) determining the nitrate and perchlorate contamination in a semi-arid plain (Harran Plain) and (2) evaluating the performance of a heterotrophic-autotrophic sequential denitrification process for nitrate and perchlorate removal from the groundwater of this plain. The nitrate in the groundwater samples varied between 4.07 and 83.22 mg l(-1) NO3--N. Perchlorate was added to groundwater samples externally and its concentration was increased from 100 to 1500 mu g l(-1). The total nitrogen concentrations in the sequential system effluent throughout the study were always below 0.5 mg l(-1). C/N ratio was 2.44 which was slightly lower than the theoretical level of 2.47. Therefore the average NO3--N in the heterotrophic reactor effluent was 19 +/- 3.7 mg l(-1) corresponding to an efficiency of 75% reduction. The remaining nitrate and nitrite were almost completely reduced in the autotrophic process. The system's perchlorate removal efficiency was above 98%, except during the last period (82%), at which influent perchlorate was 1500 mu g l(-1). The maximum perchlorate reduction rate throughout the study was around 15 mg/(L.d). Both perchlorate and nitrate reduction were partial in the heterotrophic reactor, but completed in the following autotrophic process. (C) 2016 Elsevier Ltd. All rights reserved.