Person: BİNGÖL ÖZAKPINAR, ÖZLEM
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BİNGÖL ÖZAKPINAR
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Publication Metadata only Evaluation of biochemical parameters inRubus tereticaulistreated rats and its implications in wound healing(SPRINGER, 2020) ŞEN, ALİ; Aksoy, Halil; Demirbag, Caglar; Sen, Ali; Sekerler, Turgut; Ozakpinar, Ozlem; Sener, Azize; Ahmad, Sarfraz; Tetik, SerminWe evaluated the effects ofRubus tereticaulisin healing process by determining the total carbonyl content, collagen synthesis, and total protein level on rat wounded tissues. Wounds were performed in the back of 54 Wistar rats, using a biopsy punch instrument with 0.6 mm in diameter. Rats were randomly divided into three groups: (i) un-treatment wounds group served as controls, (ii) Madecassol (R) used as positive control group, and (iii) the application of topical cream ofR. tereticaulisserved as treatment group of wound healing. The animals were killed at the end of experiment under anesthesia with ketamine, and tissue samples were collected for the evaluation at three times intervals (3rd, 7th, and 14th day). The wounded areas were analyzed for total carbonyl content, collagen, and total protein levels by HPLC, ELISA, and spectrophotometric methods, respectively. Total carbonyl content in the treatment group was significantly lower in comparison with control group on 3rd day (2.839 +/- 0.438 vs. 3.216 +/- 0.216 nmol carbonyl/mol protein;p < 0.5) and 14th days (4.222 +/- 0.128 vs. 4.784 +/- 0.077 nmol carbonyl/mol protein;p < 0.05), respectively. New collagen formation on the wound sites after the initial injury was noted in the treated and positive control groups (5.310 +/- 0.331 vs. 5.164 +/- 0.377 mg collagen/g wet tissue) at the 3rd day than control group (2.180 +/- 0.718 mg collagen/g wet tissue,p < 0.01), and in treated and positive control groups at 7th day (9.654 +/- 0.201, 9.053 +/- 1.062 mg collagen/g wet tissue,p < 0.01); and in treated and positive control groups at 14th day (8.469 +/- 0.236, 5.631 +/- 0.531 mg collagen/g wet tissue, respectively;p < 0.05) in comparison with the control group. Total protein level of samples did not change significantly between the groups. Thus, application ofR. tereticaulisameliorated the wound healing process in rats as it facilitated collagen formation through healing of the wound. Evaluating total carbonyl content by HPLC could be useful as an advance procedure for quantification of healing.Publication Metadata only Association between the growth arrest-specific 6 (Gas6) gene polymorphism c.834+7G > A and preeclampsia(TAYLOR & FRANCIS LTD, 2016) BİNGÖL ÖZAKPINAR, ÖZLEM; Ozakpinar, Ozlem Bingol; Sahin, Sadik; Verimli, Nihan; Simsek, Gulhayat Golbasi; Maurer, Anne-Marie; Eroglu, Mustafa; Tetik, Sermin; Uras, FikriyeObjective: Preeclampsia (PE) is a hypertensive disease of pregnancy complicating 2-8% of all pregnancies. The exact pathophysiology still remains unknown. Growth arrest-specific 6 (Gas6) is a member of the vitamin K-dependent protein family and it has been suggested as a novel atherothrombotic risk factor with anti-angiogenic and pro-atherogenic properties. The goal of the our study was to investigate the relationships between the c.834+7G>A polymorphism of GAS6, plasma Gas6 levels and PE.Methods: A total of 150 women, including 82 preeclamptic pregnant women and 68 normotensive pregnant (NP) women, were recruited in the current study. Blood samples were taken from all participitants. Plasma Gas6 levels measured by an enzyme-linked immunosorbent assay. GAS6 polymorphism was determined using a PCR-RFLP method.Results: The plasma Gas6 levels of preeclamptic patients were significantly lower than those of NP women (8.653.70ng/ml and 10.89 +/- 4.23ng/ml respectively, p<0.001). The GG genotype was the most prevalent, and the risk of PE was 3.5-fold higher in pregnant women with GG genotype compared to woman with AA genotype (p<0.01). The A allele was less frequent in preeclamptic patients than in control subjects (OR=2.118, 95% CI=1.330-3.371, p<0.001).Conclusions: Our results suggest that GAS6 c.834+7G>A polymorphism may have a pivotal role in the pathogenesis of PE suggesting that the A allele has a protective role for PE.Publication Metadata only The impact of platelet functions and inflammatory status on the severity of preeclampsia(TAYLOR & FRANCIS LTD, 2015) BİNGÖL ÖZAKPINAR, ÖZLEM; Sahin, Sadik; Ozakpinar, Ozlem Bingol; Eroglu, Mustafa; Tulunay, Aysin; Ciraci, Enver; Uras, Fikriye; Tetik, SerminObjective: To find out whether there is a correlation between the extent of platelet activation and inflammation and the severity of preeclampsia (PE) in the third trimester of pregnancy. Methods: Forty-one women with PE (n = 23 severe, n = 18 mild) and 80 normotensive pregnant (NP) women were included in the study. Their blood samples were obtained and interleukin (IL)-8 and IL-10 levels measured by an enzyme-linked immunosorbent assay. Basal CD61 and CD62P expressions on CD41-positive platelets were analyzed with the use of flow-cytometry. Platelet aggregation was induced by adenosine diphosphate and determined by aggregometry. Results: CD62P expression was increased in severely preeclamptic women, and the platelet aggregation was decreased in both mildly and severely preeclamptic women in comparison with NP women. However, CD61 expression was similar among the groups. An enhanced inflammatory response was seen in severely preeclamptic women demonstrated by increased levels of IL-8 and decreased levels of IL-10. However, the intensity of platelet activation did not correlate directly with the change in plasma levels of IL-8 and IL-10 in preeclamptic women. Conclusions: Platelets may have a role in the inflammatory response in PE. However, the severity of inflammation is found to be independent from the intensity of platelet activation in preeclamptic women. This seems to be related to mechanisms causing alterations of cytokine levels such as IL-8 and IL-10, rather than platelet activation.Publication Metadata only The protective effects of tacrolimus on rat uteri exposed to ischemia-reperfusion injury: a biochemical and histopathologic evaluation(ELSEVIER SCIENCE INC, 2014) AK, KORAY; Sahin, Sadik; Ozakpinar, Ozlem Bingol; Ak, Koray; Eroglu, Mustafa; Acikel, Merve; Tetik, Sermin; Uras, Fikriye; Cetinel, SuleObjective: To evaluate the effects of the immunosuppressant tacrolimus as an antioxidant and analyze the histopathologic changes in rat uteri exposed to experimental ischemia-reperfusion (I/R) injury. Design: Experimental study. Setting: Experimental surgery laboratory in a university. Animal(s): Twenty-eight female rats exposed to experimentally induced uterine I/R injury. Intervention(s): Group I: control group; group II: uterine I/R injury-induced group; group III: pre-ischemia tacrolimus group; group IV: post-ischemia tacrolimus group. Main Outcome Measure(s): Uterine tissue malondialdehyde (MDA) level as a marker of lipid peroxidation and glutathione (GSH) level and superoxide dismutase (SOD) and catalase (CAT) activities as markers of tissue antioxidant capacity; histopathologic examination of all uterine rat tissue. Result(s): Following aortic I/R injury, MDA levels were significantly increased whereas GSH levels and CAT and SOD activities were found to be decreased compared with control animals. MDA levels were found to recover prominently after the administration of tacrolimus in both groups III and IV. Administration of tacrolimus improved uterine GSH levels and CAT activity in the tacrolimus-treated groups. Conclusion(s): Our results indicate that tacrolimus reduces oxidative damage in rat uteri exposed to I/R injury induced by distal abdominal aortic occlusion. Histologic evaluation reveals that tacrolimus attenuates the inflammatory response and protects the tissue damage induced by I/R injury. (c) 2014 by American Society for Reproductive Medicine.