Person: BİNGÖL ÖZAKPINAR, ÖZLEM
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BİNGÖL ÖZAKPINAR
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ÖZLEM
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Publication Metadata only The effect of exogenous oxytocin on streptozotocin (STZ)-induced diabetic adult rat testes(ELSEVIER SCIENCE INC, 2015) BİNGÖL ÖZAKPINAR, ÖZLEM; Koroglu, P.; Senturk, G. Erkanli; Yucel, D.; Ozakpinar, O. Bingol; Uras, F.; Arbak, S.Oxytocin (OXY) plays a crucial role in reproduction. The aim of this study is to investigate the therapeutic and protective effects of oxytocin treatment on streptozotocin (STZ) induced diabetes in testicular tissue. The rats were randomly divided into four experimental groups: (I) Control Group, (II) STZ induced Diabetic Group (STZ Group), (III) STZ induced Diabetic Group with Pre-Oxytocin treatment (Pre-OXY Group) and ( IV) STZ induced Diabetic Group with Post-Oxytocin treatment (Post-OXY Group); each group contains six animals. The rats whose blood glucose levels were more than 200 mg/dl were included to the experiment. At the end of the 4th week, testes tissue samples were taken to be processed for light microscopy and transmission electron microscopy. Malondialdehyde (MDA), Glutathione (GSH) and Advanced Oxidation Protein Products (AOPP) levels were determined biochemically in blood samples. Testicular tissue samples stained with Hematoxylin and Eosin (H&E) and Periodic acid-Schiff (PAS) reaction were evaluated under light microscope. The histopathological damage score of testicular tissue, which was significantly increased in STZ group, was decreased by oxytocin treatment. According to biochemical data, MDA and AOPP levels have been increased in the blood of STZ Group compared to the Control Group whereas they decreased significantly in Oxytocin-treated Groups compared to STZ Group. GSH levels were significantly decreased in the blood of STZ Group and increased in the blood of Oxytocin-treated Groups compared to STZ Group. In conclusion, oxytocin has a potential protective effect on the testes tissue of STZ-induced diabetic rats. (C) 2014 Elsevier Inc. All rights reserved.Publication Metadata only Association between the growth arrest-specific 6 (Gas6) gene polymorphism c.834+7G > A and preeclampsia(TAYLOR & FRANCIS LTD, 2016) BİNGÖL ÖZAKPINAR, ÖZLEM; Ozakpinar, Ozlem Bingol; Sahin, Sadik; Verimli, Nihan; Simsek, Gulhayat Golbasi; Maurer, Anne-Marie; Eroglu, Mustafa; Tetik, Sermin; Uras, FikriyeObjective: Preeclampsia (PE) is a hypertensive disease of pregnancy complicating 2-8% of all pregnancies. The exact pathophysiology still remains unknown. Growth arrest-specific 6 (Gas6) is a member of the vitamin K-dependent protein family and it has been suggested as a novel atherothrombotic risk factor with anti-angiogenic and pro-atherogenic properties. The goal of the our study was to investigate the relationships between the c.834+7G>A polymorphism of GAS6, plasma Gas6 levels and PE.Methods: A total of 150 women, including 82 preeclamptic pregnant women and 68 normotensive pregnant (NP) women, were recruited in the current study. Blood samples were taken from all participitants. Plasma Gas6 levels measured by an enzyme-linked immunosorbent assay. GAS6 polymorphism was determined using a PCR-RFLP method.Results: The plasma Gas6 levels of preeclamptic patients were significantly lower than those of NP women (8.653.70ng/ml and 10.89 +/- 4.23ng/ml respectively, p<0.001). The GG genotype was the most prevalent, and the risk of PE was 3.5-fold higher in pregnant women with GG genotype compared to woman with AA genotype (p<0.01). The A allele was less frequent in preeclamptic patients than in control subjects (OR=2.118, 95% CI=1.330-3.371, p<0.001).Conclusions: Our results suggest that GAS6 c.834+7G>A polymorphism may have a pivotal role in the pathogenesis of PE suggesting that the A allele has a protective role for PE.Publication Metadata only The impact of platelet functions and inflammatory status on the severity of preeclampsia(TAYLOR & FRANCIS LTD, 2015) BİNGÖL ÖZAKPINAR, ÖZLEM; Sahin, Sadik; Ozakpinar, Ozlem Bingol; Eroglu, Mustafa; Tulunay, Aysin; Ciraci, Enver; Uras, Fikriye; Tetik, SerminObjective: To find out whether there is a correlation between the extent of platelet activation and inflammation and the severity of preeclampsia (PE) in the third trimester of pregnancy. Methods: Forty-one women with PE (n = 23 severe, n = 18 mild) and 80 normotensive pregnant (NP) women were included in the study. Their blood samples were obtained and interleukin (IL)-8 and IL-10 levels measured by an enzyme-linked immunosorbent assay. Basal CD61 and CD62P expressions on CD41-positive platelets were analyzed with the use of flow-cytometry. Platelet aggregation was induced by adenosine diphosphate and determined by aggregometry. Results: CD62P expression was increased in severely preeclamptic women, and the platelet aggregation was decreased in both mildly and severely preeclamptic women in comparison with NP women. However, CD61 expression was similar among the groups. An enhanced inflammatory response was seen in severely preeclamptic women demonstrated by increased levels of IL-8 and decreased levels of IL-10. However, the intensity of platelet activation did not correlate directly with the change in plasma levels of IL-8 and IL-10 in preeclamptic women. Conclusions: Platelets may have a role in the inflammatory response in PE. However, the severity of inflammation is found to be independent from the intensity of platelet activation in preeclamptic women. This seems to be related to mechanisms causing alterations of cytokine levels such as IL-8 and IL-10, rather than platelet activation.Publication Metadata only Reference intervals for growth arrest-specific 6 protein in adults(TAYLOR & FRANCIS LTD, 2017) AY, NADİYE PINAR; Cagman, Zeynep; Ozakpinar, Ozlem Bingol; Cirakli, Zeynep; Gedikbasi, Asuman; Ay, Pinar; Colantonio, David; Uras, Ahmet Riza; Adeli, Khosrow; Uras, FikriyeThe objective of this study was to establish reference intervals for growth arrest-specific 6 (GAS6), a vitamin K-dependent protein, in human adult plasma according to the Guideline of Clinical and Laboratory Standards Institute (CLSI) C28-A3. Blood samples were collected from 308 healthy volunteers aged 18-72 (157 female, 151 male). A non-parametric approach was used to calculate the reference interval. The plasma GAS6 reference interval was determined, with 90% confidence interval: the lower limit (2.5 percentile) was 2.5 (1.9-3.1) g/L and the upper limit (97.5 percentile)=18.8 (18.0-22.3) g/L. Harris-Boyd's test did not suggest partitioning by age or gender: medians for males [7.8 (5.8-10.7) g/L] and females [9.9 (7.1-13.5) g/L]. Three age-subgroups were tested: 18-29 years (n=168); 30-44 years (n=73); 45-72 years (n=67). The intra- and inter-assay variations were 12.6% (mean, 5.2 +/- 0.7g/L) and 14.0% (mean, 9.2 +/- 1.3g/L), respectively. The mean recovery was 104%. This study reports plasma GAS6 reference intervals established first according to the guideline of CLSI C28-A3.Publication Metadata only Obestatin alleviates subarachnoid haemorrhage-induced oxidative injury in rats via its anti-apoptotic and antioxidant effects(TAYLOR & FRANCIS LTD, 2013) ŞENER, AZİZE; Ersahin, Mehmet; Ozsavci, Derya; Sener, Azize; Ozakpinar, Ozlem Bingol; Toklu, Hale Zerrin; Akakin, Dilek; Sener, Goksel; Yegen, Berrak C.Objective: The aim was to investigate the putative anti-inflammatory and anti-apoptotic effect of obestatin in a rat model of subarachnoidal haemorrhage (SAH). Methods: To induce SAH, rats were injected with 0.3 mL blood into their cisterna magna. At 48 hours rats were decapitated after neurological examination. Blood-brain barrier (BBB) permeability, brain water content, oxidative stress markers and histological analysis were done in brain tissue. Results: The results showed that neurological examination scores were increased in the SAH group and, moreover, BBB permeability was impaired and oedema formed. SAH resulted in increased levels of plasma tumour necrosis factor (TNF)-alpha, interleukin (IL)-1 beta, IL-6 levels and caspase-3 activity. Lipid peroxidation and protein oxidation levels and myeloperoxidase activity were all increased in the brain tissue, with concomitant decreases in antioxidant enzymes. On the other hand, SAH-induced neurological impairment and oxidative brain injury were ameliorated in the obestatin-treated group. Conclusion: The present study provides the first evidence that peripheral administration of obestatin exerts potent anti-inflammatory and neuroprotective effects in SAH-induced oxidative damage by maintaining a balance in oxidant-antioxidant status through the augmentation of endogenous antioxidants and the inhibition of pro-inflammatory mediators.