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YARAT, AYŞEN

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Diş Hekimliği Fakültesi

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YARAT

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AYŞEN

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Open Access
Clinical evaluation of dental enamel defects and oral findings in coeliac children
(ISTANBUL UNIV PRESS, ISTANBUL UNIV RECTORATE, 2019-01-23) YARAT, AYŞEN; Bicak, Damla Aksit; Urganci, Nafiye; Akyuz, Serap; Usta, Merve; Kizilkan, Nuray Uslu; Alev, Burcin; Yarat, Aysen
Purpose To examine dental hard and soft tissue changes of coeliac children in order to increase the awareness of the pediatric dentists in prediagnosis of especially undiagnosed coeliac disease. Materials and methods Sixty children, 28 (46.7%) boys and 32 (53.3%) girls whose ages were between 6 to 16 years were included in the present study. Thirty children who had undergone endoscopy and diagnosed with the coeliac disease in the Sisli Hamidiye Etfal Hospital, Istanbul, Turkey, formed the study group. Also, thirty children clinically suspected of having the coeliac disease with the same gastrointestinal complaints had undergone endoscopy and proven not coeliac were chosen as the control group. Oral examination involved assessment of dentition and specific and unspecific dental enamel defects. Also, soft tissue lesions, clinical delay of the dental eruption, salivary flow rate, pH, and buffering capacity were examined. Results Twenty coeliac patients had enamel defects, however none in the control subjects. In the coeliac group, all enamel defects were diagnosed in permanent teeth and as specific in all children. Grade I dental enamel defects found mainly in the incisors. The clinical delayed eruption was observed in 10 (33.3%) of 30 coeliac children and none of the children in the control group. While the level of DMFT/S numbers and stimulated salivary flow rate were found significantly lower in the coeliac group, pH was found significantly higher. Conclusion Oral cavity may be involved in coeliac disease and pediatric dentists can play an important role in the early diagnosis of the coeliac disease.
Open Access
Tooth replantation with adipose tissue stem cells and fibrin sealant: microscopic analysis of rat's teeth
(SPRINGER INTERNATIONAL PUBLISHING AG, 2016-12) YARAT, AYŞEN; Demirel, Sezin; Yalvac, Mehmet Emir; Tapsin, Sidika; Akyuz, Serap; Ak, Esin; Cetinel, Sule; Yarat, Aysen; Sahin, Fikrettin
Treatment for dental avulsion cases is early or late replantation of the traumatized teeth. Prognosis of the replanted tooth depends on the level of periodontal injury. Adipose tissue stem cells (ATSCs) were reported to improve periodontal ligament tissue (PDL) regeneration. Fibrin sealant (FS) contains thrombin and fibrinogen to form an adhesive fibrin clot routinely used in surgical procedures. Here, we aimed to investigate the effects of ATSCs + FS treatment on healing of PDL after tooth replantation in a rat model. After 60 min of extraction, maxillary central incisor teeth were replanted with ATSCs + FS. Two months later, the rats were sacrificed and hemimaxilla blocks were dissected out for histological analysis. The results showed that there was a significant improvement in histological findings of ATSCs + FS treated group compared to only FS treated and non-treated groups corresponding to reduced inflammatory resorption and increased new PDL formation. Furthermore, the ankylosis levels were lowered after ATSCs + FS treatment. Singular use of FS improved PDL healing moderately. Our results indicated that ATSCs + FS treatment improves PDL healing after tooth replantation suggesting a new therapeutic potential in the treatment of dental avulsion cases.
Open Access
Edaravone Ameliorates Valproate-Induced Gingival Toxicity by Reducing Oxidative-Stress, Inflammation and Tissue Damage
(MARMARA UNIV, FAC MEDICINE, 2016-05-10) YARAT, AYŞEN; Oktay, Sehkar; Alev, Burcin; Koc Ozturk, Leyla; Tunali, Sevim; Demirel, Sezin; Emekli Alturfan, Ebru; Tunali-Akbay, Tugba; Akyuz, Serap; Yanardag, Refiye; Yarat, Aysen
Valproic acid (2-n-propylpentanoic acid, VPA), the most widely used antiepileptic drug, has potential adverse effects and it can disrupt the oxidant and antioxidant balance. Edaravone (3-methyl-1-phenyl-2-pyrazoline-5-one, EDA) is a potent free radical scavenger. In this study, the effect of EDA on gingiva in VPA induced toxicity was investigated. Female Sprague Dawley rats were randomly divided into four groups: control group, EDA (30 mg/kg/day) given group, VPA (0.5 g/kg/day) given group, and VPA+EDA (in same dose and time) given group. EDA and VPA were given intraperitoneally for seven days. Total protein, lipid peroxidation (LPO), sialic acid (SA) and reduced glutathione (GSH) levels and catalase (CAT), glutathione-S-transferase (GST), glutathione peroxidase (GPx), superoxide dismutase (SOD), myeloperoxidase (MPO), alkaline phosphatase (ALP), acid phosphatase (ACP), sodium potassium ATPase (Na+/K+-ATPase) and tissue factor (TF) activities were determined in gingiva homogenates. The VPA-induced increases were statistically significant for MPO (p<0.01), ACP (p<0.01), Na+/K+-ATPase (p<0.05) and TF (p<0.01) activities, but not for LPO level and ALP activities. EDA treatment markedly blunted all such elevated anomalies. Conclusively, VPA induced oxidative and inflammatory gingival tissue damage, reactions that were appreciably reversed by concurrent administration of EDA.
Open Access
In Vitro Fluoride-Release/Recharge Pattern and_x000D_ Antimicrobial Effects of Current Restorative_x000D_ Materials Used in Pediatric Dentistry
(2020-04-13) YARAT, AYŞEN; Belen Şirinoğlu ÇAPAN;Serap AKYÜZ;Burçin ALEV;Beste TACAL ASLAN;Tanju KADİR;Ayşen YARAT
Objective: The aim of this in vitro study was to investigate the fluoride-releasing/recharging and antimicrobial effects of restorative materialsused in pediatric dentistry.Material and Method: High-viscosity and resin-modified glass-ionomercement, glass carbomer, compomer, giomer and composite weretested. Dental materials’ fluoride-release was measured in distilled water(DW) and artificial saliva (AS) for eight weeks using a fluoride-specificelectrode. At the end of eight weeks, specimens were divided intothree groups for recharging with acidulated phosphate fluoride gel andfluoride varnish. After recharge, fluoride release was measured again.Agar diffusion test was used to assess antimicrobial effects againstS. mutans and L. acidophilus. The statistical analysis was done withMann-Whitney U and Friedman tests using SPSS-22 software.Results: High-viscosity glass-ionomer cement showed the highestfluoride-release in both storage media, followed by glass carbomer,while giomer demonstrated the lowest. After recharge, the increasein the varnish group was significantly higher than others (p<0.05).Fluoride-release in DW groups was significantly higher than in the ASgroups for all materials during the test period (p<0.05). None of thematerials showed antimicrobial effects.Conclusion: It was concluded that fluoride-release from glass ionomer-based materials is higher than that from resin-based materials.Fluoride-release from materials decreases in AS. Glass carbomer can beused as an alternative to glass ionomers.
Open Access
The investigation of Helicobacter pylori in the dental biofilm and saliva samples of children with dyspeptic complaints
(BMC, 2017-12) YARAT, AYŞEN; Bicak, Damla Aksit; Akyuz, Serap; Kiratli, Binnur; Usta, Merve; Urganci, Nafiye; Alev, Burcin; Yarat, Aysen; Sahin, Fikrettin
Background: The oral cavity can be an extra-gastric reservoir for Helicobacter pylori (H. pylori). This can play a role in the pathogenesis of halitosis, glossitis, recurrent aphthous stomatitis, and dental caries. The present study was conducted to detect the presence of H. pylori within the dental biofilm and in saliva samples collected from children suffering from dyspepsia and children without any gastrointestinal complaints. Associations with gastric infection, halitosis, and some oral parameters were also evaluated. Methods: Seventy children (aged between 5-16) with dyspepsia were selected for the study group and control group composed of 30 healthy children without dyspepsia were also included in the study. After detailed oral and clinical examinations for oral parameters, saliva, and supragingival dental biofilm samples were collected for 16S rRNA and 23S rRNA genes detection by real-time polymerase chain reaction (RT-PCR). The presence of gastric H. pylori was evaluated in endoscopic biopsy specimens histopathologically. Halitosis was evaluated by benzoylDL-arginine-naphthylamid (BANA) test. Salivary S. mutans and Lactobacilli sp. counts were also carried out by commercial kits. Results: H. pylori was histopathologically detected amongst 83% of the children with the dyspeptic condition. The detection rate of this bacteria in dental biofilm and saliva samples and halitosis were found relatively higher in the dyspeptic children rather than the control group (p < 0.01). Halitosis was not significantly different between dyspeptic children and those detected with H. pylori (p > 0.05). In the gastric H. pylori positive group with dyspepsia, DMFT/ S and dmft/ s numbers and plaque indices were found higher than the control group (p < 0.01). Only plaque indices of gastric H. pylori negative group with dyspepsia were found higher than the control group (p < 0.01). S. mutans and Lactobacilli sp. counts were not significantly different between gastric H. pylori positive and negative groups (p > 0.05). Comparing to those with negative for both genes, in children whose dental biofilm and saliva samples were positive for both 16S rRNA and 23S rRNA genes, significantly higher results for halitosis, and DMFS numbers and significantly lower results for dmfs numbers and pH values were found (p < 0.01). Conclusions: Helicobacter pylori can occur in the oral cavity aside and independently from the stomach. However, the high number of bacteria in the oral cavities of children with gastric H. pylori, an association between the presence of H. pylori and halitosis, DMFS, and pH were found.
Open Access
The level of two trace elements in carious, non-carious, primary, and permanent teeth
(ISTANBUL UNIV PRESS, ISTANBUL UNIV RECTORATE, 2020-08-13) YARAT, AYŞEN; Kuru, Ruya; Balan, Gulsah; Yilmaz, Sahin; Tasli, Pakize Neslihan; Akyuz, Serap; Yarat, Aysen; Sahin, Fikrettin
Purpose The boron and fluoride mainly accumulate in the bones and teeth of the human body. The purpose of this study is to determine boron or fluoride levels in the whole tooth, to evaluate the correlation between their levels and to compare these levels in primary/permanent, carious, and non-carious groups. Materials and Methods The boron and fluoride levels of thirty-six teeth, separated such as primary carious (n=9) and non-carious (n=9), permanent carious (n=9) and non-carious (n=9), were determined by ICP-MS and ion-selective electrode, respectively. Results While boron levels were between 0.001 and 5.88 ppm, the fluoride levels were between 21.24 and 449.22 ppm. The boron level of non- carious teeth was higher than those of carious teeth in primary and permanent tooth groups. However, this difference was not statistically significant (p>0.05). The fluoride level of non-carious teeth was higher than those of carious teeth in primary (p=0.062) and permanent teeth groups (p=0.046). Negative correlation, found between boron and fluoride in all groups, was significant only in non-carious teeth group (r=-0.488, p=0.040). Conclusion The results of our study proved the importance of fluoride as a protective factor for dental caries once more. The boron levels in non- carious teeth were also higher than carious teeth. However, it was not significant. Moreover, there was negative correlation between teeth boron and fluoride levels. Therefore, it is necessary to conduct more detailed studies on the tooth boron level and its relation with caries formation and with fluoride levels.
Open Access
INVESTIGATION OF THE N-TERMINAL CODING REGION OF MUC7 ALTERATIONS IN DENTISTRY STUDENTS WITH AND WITHOUT CARIES
(MACEDONIAN ACAD SCIENCES ARTS, 2016-06-01) YARAT, AYŞEN; Ozturk, Koc L.; Yarat, A.; Akyuz, S.; Furuncuoglu, H.; Ulucan, K.
Human low-molecular weight salivary mucin (MUC7) is a small, secreted glycoprotein coded by MUC7. In the oral cavity, they inhibit the colonization of oral bacteria, including cariogenic ones, by masking their surface adhesions, thus helping saliva to avoid dental caries. The N-terminal domain is important for low-molecular weight (MG2) mucins to contact with oral microorganisms. In this study, we aimed to identify the N-terminal coding region of the MUC7 gene between individuals with and without caries. Forty-four healthy dental students were enrolled in this study; 24 of them were classified to have caries [decayed, missing, filled-teeth (DMFT) = 5.6] according to the World Health Organization (WHO) criteria, and 20 of them were caries-free (DMFT = 0). Simplified oral hygiene index (OHI-S) and gingival index (GI) were used to determine the oral hygiene and gingival conditions. Total protein levels and salivary total protein levels and salivary buffer capacity (SBC) were determined by Lowry and Ericsson methods. DNA was extracted from peripheral blood cells of all the participants and genotyping was carried out by a polymerase chain reaction (PCR)-sequencing method. No statistical differences were found between two groups in the terms of salivary parameters, oral hygiene and gingival conditions. We detected one common single nucleotide polymorphism (SNP) that leads to a change of asparagine to lysine at codon 80. This substitution was found in 29.0 and 40.0%, respectively, of the groups with and without caries. No other sequence variations were detected. The SNP found in this study may be a specific polymorphism affecting the Turkish population. Further studies with extended numbers are necessary in order to clarify this finding.
Open Access
Bir Akut Lenfoblastik Lösemi (ALL) Olgusunun Tedavi Sırasında Tükürük Tromboplastik Aktivitesinin Değerlendirilmesi
(2014-05-13) YARAT, AYŞEN