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YARAT, AYŞEN

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2013 - 20193
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Author: AKYÜZ, SERAP HATİCE × Subject: IN-VITRO ×

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    Isolation and Characterization of Dental Pulp Stem Cells from a Patient with Papillon-Lefevre Syndrome
    (ELSEVIER SCIENCE INC, 2013) YARAT, AYŞEN; Tasli, Pakize Neslihan; Tapsin, Sidika; Demirel, Sezin; Yalvac, Mehmet Emir; Akyuz, Serap; Yarat, Aysen; Sahin, Fikrettin
    Introduction: Papillon-Lefevre syndrome (PLS) is a rare autosomal recessive disorder. characterized by immune dysregulation because of a mutation in cathepsin c gene, resulting in hyperkeratosis of the palms, soles, elbows, and knees combined with premature loss of the primary and permanent dentitions. Periodontal tissue abnormalities in PLS patients were reported previously. However, less is known about dental pulp tissue derived cells of PLS patients. This study aimed to show stem cell potential of PLS dental pulp stem cells (DPSCs) and provide new evidence regarding the pathophysiology of the disease. Methods: DPSCs were characterized by using flow cytometry and immunocytochemistry. They were also induced to differentiate into adipogenic, osteogenic, chondrogenic, odontogenic, and myogenic cells. Results: The results revealed that PLS DPSCs are stained positive for mesenchymal stem cells surface markers CD29, CD73, CD90, CD105, and CD166. PLS DPSCs were able to differentiate into adipogenic, osteogenic, chondrogenic, and odontogenic cell types properly. PLS DPSCs expressed embryonic stem cell markers Oct4, Sox2, cMYc, and Klf4 and showed similar proliferation rate compared with DPSCs isolated from healthy young controls. Interestingly, it was found that unlike the healthy DPSCs, PLS DPSCs are not able to form myotubes with correct morphology. Conclusions: These data are being reported for the first time; therefore, they might provide new insights to the pathology of the disease. Our results suggest that the PLS DPSCs might be an autologous stem cell source for PIS patients for cellular therapy of alveolar bone defects and other dental tissue-abnormalities observed in PLS. (J Endod 2013;39:31-38)
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    Edaravone Ameliorates Valproate-Induced Gingival Toxicity by Reducing Oxidative-Stress, Inflammation and Tissue Damage
    (MARMARA UNIV, FAC MEDICINE, 2016-05-10) YARAT, AYŞEN; Oktay, Sehkar; Alev, Burcin; Koc Ozturk, Leyla; Tunali, Sevim; Demirel, Sezin; Emekli Alturfan, Ebru; Tunali-Akbay, Tugba; Akyuz, Serap; Yanardag, Refiye; Yarat, Aysen
    Valproic acid (2-n-propylpentanoic acid, VPA), the most widely used antiepileptic drug, has potential adverse effects and it can disrupt the oxidant and antioxidant balance. Edaravone (3-methyl-1-phenyl-2-pyrazoline-5-one, EDA) is a potent free radical scavenger. In this study, the effect of EDA on gingiva in VPA induced toxicity was investigated. Female Sprague Dawley rats were randomly divided into four groups: control group, EDA (30 mg/kg/day) given group, VPA (0.5 g/kg/day) given group, and VPA+EDA (in same dose and time) given group. EDA and VPA were given intraperitoneally for seven days. Total protein, lipid peroxidation (LPO), sialic acid (SA) and reduced glutathione (GSH) levels and catalase (CAT), glutathione-S-transferase (GST), glutathione peroxidase (GPx), superoxide dismutase (SOD), myeloperoxidase (MPO), alkaline phosphatase (ALP), acid phosphatase (ACP), sodium potassium ATPase (Na+/K+-ATPase) and tissue factor (TF) activities were determined in gingiva homogenates. The VPA-induced increases were statistically significant for MPO (p<0.01), ACP (p<0.01), Na+/K+-ATPase (p<0.05) and TF (p<0.01) activities, but not for LPO level and ALP activities. EDA treatment markedly blunted all such elevated anomalies. Conclusively, VPA induced oxidative and inflammatory gingival tissue damage, reactions that were appreciably reversed by concurrent administration of EDA.
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    Antibacterial and photodynamic effects of some plant extracts for cavity disinfection
    (ELSEVIER, 2019) YARAT, AYŞEN; Akyuz, Serap; Chousein (Ntemir), Ozlem Moufti; Sacan, Ozlem; Yanardag, Refiye; Kalayci, Sadik; Yarat, Aysen; Sahin, Fikrettin
    Background: In current dental treatments, with the aim of a preventive approach,it is argued that removing only the infected layer of dentin is sufficient for cavity preparation. However it is impossible to be sure that the infected layer was completely removed. In addition, the cause of secondary caries and post operative sensitivities has been reported as residual bacteria in some studies. The aim of this study is to investigate the antibacterial and photo-active properties of Cotinus coggygria Scop., Rumex cristatus DC., Beta vulgaris L.var.cicla and Eruca sativa aqueous extracts, and to investigate their usefulness for cavity disinfection in dentistry. Method: The aqueous solutions of plant extracts were prepared to be at a maximum concentration and the Streptococcus mutans solutions mixed with phosphate buffered saline to give 10(8) cfu/mL. A 430-480 nm wavelength light source was used for the irradiation. Three different applications were made: extract + Streptococcus mutans mixture exposed to ligh; extract + Streptococcus mutans mixture that was not exposed to light and S. mutans exposed to light. Results: No antibacterial effect was found for the second and third applications. In the first application, however, irradiation with extract + Streptococcus mutans mixture reduced the number of microorganisms in the beginning by 99% for only Rumex cristatus DC. extract (log 2). Conclusion. Rumex cristatus DC. extract can be used as art alternative in photo-active disinfection of cavities in dentistry.