Person: YARAT, AYŞEN
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YARAT
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AYŞEN
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Publication Open Access Clinical evaluation of dental enamel defects and oral findings in coeliac children(ISTANBUL UNIV PRESS, ISTANBUL UNIV RECTORATE, 2019-01-23) YARAT, AYŞEN; Bicak, Damla Aksit; Urganci, Nafiye; Akyuz, Serap; Usta, Merve; Kizilkan, Nuray Uslu; Alev, Burcin; Yarat, AysenPurpose To examine dental hard and soft tissue changes of coeliac children in order to increase the awareness of the pediatric dentists in prediagnosis of especially undiagnosed coeliac disease. Materials and methods Sixty children, 28 (46.7%) boys and 32 (53.3%) girls whose ages were between 6 to 16 years were included in the present study. Thirty children who had undergone endoscopy and diagnosed with the coeliac disease in the Sisli Hamidiye Etfal Hospital, Istanbul, Turkey, formed the study group. Also, thirty children clinically suspected of having the coeliac disease with the same gastrointestinal complaints had undergone endoscopy and proven not coeliac were chosen as the control group. Oral examination involved assessment of dentition and specific and unspecific dental enamel defects. Also, soft tissue lesions, clinical delay of the dental eruption, salivary flow rate, pH, and buffering capacity were examined. Results Twenty coeliac patients had enamel defects, however none in the control subjects. In the coeliac group, all enamel defects were diagnosed in permanent teeth and as specific in all children. Grade I dental enamel defects found mainly in the incisors. The clinical delayed eruption was observed in 10 (33.3%) of 30 coeliac children and none of the children in the control group. While the level of DMFT/S numbers and stimulated salivary flow rate were found significantly lower in the coeliac group, pH was found significantly higher. Conclusion Oral cavity may be involved in coeliac disease and pediatric dentists can play an important role in the early diagnosis of the coeliac disease.Publication Open Access Tooth replantation with adipose tissue stem cells and fibrin sealant: microscopic analysis of rat's teeth(SPRINGER INTERNATIONAL PUBLISHING AG, 2016-12) YARAT, AYŞEN; Demirel, Sezin; Yalvac, Mehmet Emir; Tapsin, Sidika; Akyuz, Serap; Ak, Esin; Cetinel, Sule; Yarat, Aysen; Sahin, FikrettinTreatment for dental avulsion cases is early or late replantation of the traumatized teeth. Prognosis of the replanted tooth depends on the level of periodontal injury. Adipose tissue stem cells (ATSCs) were reported to improve periodontal ligament tissue (PDL) regeneration. Fibrin sealant (FS) contains thrombin and fibrinogen to form an adhesive fibrin clot routinely used in surgical procedures. Here, we aimed to investigate the effects of ATSCs + FS treatment on healing of PDL after tooth replantation in a rat model. After 60 min of extraction, maxillary central incisor teeth were replanted with ATSCs + FS. Two months later, the rats were sacrificed and hemimaxilla blocks were dissected out for histological analysis. The results showed that there was a significant improvement in histological findings of ATSCs + FS treated group compared to only FS treated and non-treated groups corresponding to reduced inflammatory resorption and increased new PDL formation. Furthermore, the ankylosis levels were lowered after ATSCs + FS treatment. Singular use of FS improved PDL healing moderately. Our results indicated that ATSCs + FS treatment improves PDL healing after tooth replantation suggesting a new therapeutic potential in the treatment of dental avulsion cases.Publication Metadata only Salivary lipid peroxidation and total sialic acid levels during healthy gestation and postpartum: A longitudinal study(PERGAMON-ELSEVIER SCIENCE LTD, 2010) YARAT, AYŞEN; Ozturk, L. Koc; Akyuz, S.; Yarat, A.; Koc, S.; Gul, N.; Dogan, B. N.Objectives: This study investigated salivary lipid peroxidation (LPO) as an oxidative stress marker and salivary total sialic acid (TSA) as an inflammatory response during gestation and postpartum. Design and methods: Salivary LPO and TSA levels, using the Ledwozyw and Warren methods respectively, were obtained in healthy pregnant women followed up during gestation and 6-8 weeks postpartum, and in healthy non-pregnant controls. All were with good oral health. Results: LPO was significantly higher than controls during all trimesters and postpartum and in the second trimester than in the third trimester and postpartum. TSA in the second trimester was significantly higher than in any other group. First trimester levels were significantly higher than postpartum. Oral health indices remained within normal levels for the duration. Conclusion: The salivary LPO profile followed plasma gestation and postpartum profiles in the literature but the salivary TSA differed in that after the 2nd trimester, rather than persisting, it decreased. (C) 2009 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.Publication Metadata only Isolation and Characterization of Dental Pulp Stem Cells from a Patient with Papillon-Lefevre Syndrome(ELSEVIER SCIENCE INC, 2013) YARAT, AYŞEN; Tasli, Pakize Neslihan; Tapsin, Sidika; Demirel, Sezin; Yalvac, Mehmet Emir; Akyuz, Serap; Yarat, Aysen; Sahin, FikrettinIntroduction: Papillon-Lefevre syndrome (PLS) is a rare autosomal recessive disorder. characterized by immune dysregulation because of a mutation in cathepsin c gene, resulting in hyperkeratosis of the palms, soles, elbows, and knees combined with premature loss of the primary and permanent dentitions. Periodontal tissue abnormalities in PLS patients were reported previously. However, less is known about dental pulp tissue derived cells of PLS patients. This study aimed to show stem cell potential of PLS dental pulp stem cells (DPSCs) and provide new evidence regarding the pathophysiology of the disease. Methods: DPSCs were characterized by using flow cytometry and immunocytochemistry. They were also induced to differentiate into adipogenic, osteogenic, chondrogenic, odontogenic, and myogenic cells. Results: The results revealed that PLS DPSCs are stained positive for mesenchymal stem cells surface markers CD29, CD73, CD90, CD105, and CD166. PLS DPSCs were able to differentiate into adipogenic, osteogenic, chondrogenic, and odontogenic cell types properly. PLS DPSCs expressed embryonic stem cell markers Oct4, Sox2, cMYc, and Klf4 and showed similar proliferation rate compared with DPSCs isolated from healthy young controls. Interestingly, it was found that unlike the healthy DPSCs, PLS DPSCs are not able to form myotubes with correct morphology. Conclusions: These data are being reported for the first time; therefore, they might provide new insights to the pathology of the disease. Our results suggest that the PLS DPSCs might be an autologous stem cell source for PIS patients for cellular therapy of alveolar bone defects and other dental tissue-abnormalities observed in PLS. (J Endod 2013;39:31-38)Publication Open Access Edaravone Ameliorates Valproate-Induced Gingival Toxicity by Reducing Oxidative-Stress, Inflammation and Tissue Damage(MARMARA UNIV, FAC MEDICINE, 2016-05-10) YARAT, AYŞEN; Oktay, Sehkar; Alev, Burcin; Koc Ozturk, Leyla; Tunali, Sevim; Demirel, Sezin; Emekli Alturfan, Ebru; Tunali-Akbay, Tugba; Akyuz, Serap; Yanardag, Refiye; Yarat, AysenValproic acid (2-n-propylpentanoic acid, VPA), the most widely used antiepileptic drug, has potential adverse effects and it can disrupt the oxidant and antioxidant balance. Edaravone (3-methyl-1-phenyl-2-pyrazoline-5-one, EDA) is a potent free radical scavenger. In this study, the effect of EDA on gingiva in VPA induced toxicity was investigated. Female Sprague Dawley rats were randomly divided into four groups: control group, EDA (30 mg/kg/day) given group, VPA (0.5 g/kg/day) given group, and VPA+EDA (in same dose and time) given group. EDA and VPA were given intraperitoneally for seven days. Total protein, lipid peroxidation (LPO), sialic acid (SA) and reduced glutathione (GSH) levels and catalase (CAT), glutathione-S-transferase (GST), glutathione peroxidase (GPx), superoxide dismutase (SOD), myeloperoxidase (MPO), alkaline phosphatase (ALP), acid phosphatase (ACP), sodium potassium ATPase (Na+/K+-ATPase) and tissue factor (TF) activities were determined in gingiva homogenates. The VPA-induced increases were statistically significant for MPO (p<0.01), ACP (p<0.01), Na+/K+-ATPase (p<0.05) and TF (p<0.01) activities, but not for LPO level and ALP activities. EDA treatment markedly blunted all such elevated anomalies. Conclusively, VPA induced oxidative and inflammatory gingival tissue damage, reactions that were appreciably reversed by concurrent administration of EDA.Publication Metadata only The investigation of genetic polymorphisms in the carbonic anhydrase VI gene exon 2 and salivary parameters in type 2 diabetic patients and healthy adults(SPRINGER, 2012) YARAT, AYŞEN; Ozturk, Leyla Koc; Ulucan, Korkut; Akyuz, Serap; Furuncuoglu, Halit; Bayer, Hikmet; Yarat, AysenThe aim of this study was to investigate carbonic anhydrase (CA) VI Exon 2 single nucleotide polymorphism (SNP) and its possible association with salivary parameters in type 2 diabetic patients compared to healthy adults. Caries status was measured by using the DMFT (number of decayed, missing, and filled teeth) index. Unstimulated whole saliva and blood samples were taken. SNPs of CA gene exon 2 were determined by PCR and DNA sequencing. Salivary CA activity and buffering capacity were determined by the method of Verpoorte and Ericson, respectively. Furthermore, salivary pH was measured with pH paper and salivary flow rate was calculated. Salivary buffering capacity and pH were significantly lower in diabetic patients than those of healthy subjects (P < 0.05). Salivary flow rate, CA activity and DMFT levels did not differ between groups (P > 0.05). Four SNPs were detected; their pubmed database number are rs2274327 (C/T), rs2274328 (A/C), rs2274329 (G/C) and rs2274330. While first three of those were responsible for amino acid changes, the last one was not. The frequencies of SNPs were not significant between groups (P > 0.05). Positive significant correlation was found between CA activity and the frequency of SNPs. There was no correlation between the SNPs frequencies and pH or buffering capacity. SNPs found in this study may be related to salivary CA activity in diabetics.Publication Metadata only Factors Influencing Career Choices of Dental Students in a State University in Turkey(MARMARA UNIV, INST HEALTH SCIENCES, 2018) YARAT, AYŞEN; Capan, Belen Sirinoglu; Akyuz, Serap; Bahcecik, Nefise; Yarat, Aysen; Girgin, FatihObjective: The aim of this study was to investigate the factors influencing the career choices of students in Marmara University Faculty of Dentistry in the 2014-2015 academic year. Methods: Data were collected from 468 dental students by using a 55-item questionnaire. Results: Mean age of students were 21.6 (range 17-25); 300(64.1%) of them were female and 168(35.9%) were male. Two hundred two (43.2%) students indicated that their first choice was dentistry. If they were to make a choice for a second time, 196(41.9%) of students would again prefer dentistry as their career. There was a significant difference between the year of study and the statement I will prefer dentistry again (p<0.05). In fact, 45(49.5%) of first-year students claimed that they would choose dentistry again, whereas 35(41.7%) of fifth-year students stated the opposite. As being passed to higher classes, the percentage of students who will prefer dentistry again decreased. One hundred forty one (30.1%) of all students reported wanting to change their career. It was stated as reasons for changing careers that being a dental student was stressful. Students were most influenced by their family (61.4%). Four hundred eight (87.2%) students were determined to choose dentistry because of its high income. 399 (85.2%) students stated that dentistry is a prestigious profession and this criterion was significant in their career choices. Conclusion: The majority of students who preferred Marmara University, Faculty of Dentistry were girls, and there were many factors influence the career choices and future plans of the students, with the most important beings were the families, the high income level and the prestige of the profession.Publication Open Access In Vitro Fluoride-Release/Recharge Pattern and_x000D_ Antimicrobial Effects of Current Restorative_x000D_ Materials Used in Pediatric Dentistry(2020-04-13) YARAT, AYŞEN; Belen Şirinoğlu ÇAPAN;Serap AKYÜZ;Burçin ALEV;Beste TACAL ASLAN;Tanju KADİR;Ayşen YARATObjective: The aim of this in vitro study was to investigate the fluoride-releasing/recharging and antimicrobial effects of restorative materialsused in pediatric dentistry.Material and Method: High-viscosity and resin-modified glass-ionomercement, glass carbomer, compomer, giomer and composite weretested. Dental materials’ fluoride-release was measured in distilled water(DW) and artificial saliva (AS) for eight weeks using a fluoride-specificelectrode. At the end of eight weeks, specimens were divided intothree groups for recharging with acidulated phosphate fluoride gel andfluoride varnish. After recharge, fluoride release was measured again.Agar diffusion test was used to assess antimicrobial effects againstS. mutans and L. acidophilus. The statistical analysis was done withMann-Whitney U and Friedman tests using SPSS-22 software.Results: High-viscosity glass-ionomer cement showed the highestfluoride-release in both storage media, followed by glass carbomer,while giomer demonstrated the lowest. After recharge, the increasein the varnish group was significantly higher than others (p<0.05).Fluoride-release in DW groups was significantly higher than in the ASgroups for all materials during the test period (p<0.05). None of thematerials showed antimicrobial effects.Conclusion: It was concluded that fluoride-release from glass ionomer-based materials is higher than that from resin-based materials.Fluoride-release from materials decreases in AS. Glass carbomer can beused as an alternative to glass ionomers.Publication Open Access The investigation of Helicobacter pylori in the dental biofilm and saliva samples of children with dyspeptic complaints(BMC, 2017-12) YARAT, AYŞEN; Bicak, Damla Aksit; Akyuz, Serap; Kiratli, Binnur; Usta, Merve; Urganci, Nafiye; Alev, Burcin; Yarat, Aysen; Sahin, FikrettinBackground: The oral cavity can be an extra-gastric reservoir for Helicobacter pylori (H. pylori). This can play a role in the pathogenesis of halitosis, glossitis, recurrent aphthous stomatitis, and dental caries. The present study was conducted to detect the presence of H. pylori within the dental biofilm and in saliva samples collected from children suffering from dyspepsia and children without any gastrointestinal complaints. Associations with gastric infection, halitosis, and some oral parameters were also evaluated. Methods: Seventy children (aged between 5-16) with dyspepsia were selected for the study group and control group composed of 30 healthy children without dyspepsia were also included in the study. After detailed oral and clinical examinations for oral parameters, saliva, and supragingival dental biofilm samples were collected for 16S rRNA and 23S rRNA genes detection by real-time polymerase chain reaction (RT-PCR). The presence of gastric H. pylori was evaluated in endoscopic biopsy specimens histopathologically. Halitosis was evaluated by benzoylDL-arginine-naphthylamid (BANA) test. Salivary S. mutans and Lactobacilli sp. counts were also carried out by commercial kits. Results: H. pylori was histopathologically detected amongst 83% of the children with the dyspeptic condition. The detection rate of this bacteria in dental biofilm and saliva samples and halitosis were found relatively higher in the dyspeptic children rather than the control group (p < 0.01). Halitosis was not significantly different between dyspeptic children and those detected with H. pylori (p > 0.05). In the gastric H. pylori positive group with dyspepsia, DMFT/ S and dmft/ s numbers and plaque indices were found higher than the control group (p < 0.01). Only plaque indices of gastric H. pylori negative group with dyspepsia were found higher than the control group (p < 0.01). S. mutans and Lactobacilli sp. counts were not significantly different between gastric H. pylori positive and negative groups (p > 0.05). Comparing to those with negative for both genes, in children whose dental biofilm and saliva samples were positive for both 16S rRNA and 23S rRNA genes, significantly higher results for halitosis, and DMFS numbers and significantly lower results for dmfs numbers and pH values were found (p < 0.01). Conclusions: Helicobacter pylori can occur in the oral cavity aside and independently from the stomach. However, the high number of bacteria in the oral cavities of children with gastric H. pylori, an association between the presence of H. pylori and halitosis, DMFS, and pH were found.Publication Open Access The level of two trace elements in carious, non-carious, primary, and permanent teeth(ISTANBUL UNIV PRESS, ISTANBUL UNIV RECTORATE, 2020-08-13) YARAT, AYŞEN; Kuru, Ruya; Balan, Gulsah; Yilmaz, Sahin; Tasli, Pakize Neslihan; Akyuz, Serap; Yarat, Aysen; Sahin, FikrettinPurpose The boron and fluoride mainly accumulate in the bones and teeth of the human body. The purpose of this study is to determine boron or fluoride levels in the whole tooth, to evaluate the correlation between their levels and to compare these levels in primary/permanent, carious, and non-carious groups. Materials and Methods The boron and fluoride levels of thirty-six teeth, separated such as primary carious (n=9) and non-carious (n=9), permanent carious (n=9) and non-carious (n=9), were determined by ICP-MS and ion-selective electrode, respectively. Results While boron levels were between 0.001 and 5.88 ppm, the fluoride levels were between 21.24 and 449.22 ppm. The boron level of non- carious teeth was higher than those of carious teeth in primary and permanent tooth groups. However, this difference was not statistically significant (p>0.05). The fluoride level of non-carious teeth was higher than those of carious teeth in primary (p=0.062) and permanent teeth groups (p=0.046). Negative correlation, found between boron and fluoride in all groups, was significant only in non-carious teeth group (r=-0.488, p=0.040). Conclusion The results of our study proved the importance of fluoride as a protective factor for dental caries once more. The boron levels in non- carious teeth were also higher than carious teeth. However, it was not significant. Moreover, there was negative correlation between teeth boron and fluoride levels. Therefore, it is necessary to conduct more detailed studies on the tooth boron level and its relation with caries formation and with fluoride levels.