Person: YARAT, AYŞEN
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YARAT
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AYŞEN
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Publication Metadata only Kronik periodontitisli ve sağlıklı kişilerin tükürük örneklerinin bazı biyokimyasal parametreler ve sitolojik incelemeler açısından karşılaştırılmasında saklama süresinin belirlenmesi(2009-06-20) ALTURFAN, EBRU IŞIK; YARAT, AYŞEN; AK, ESİN; ALTURFAN E. I., YARAT A., AK E., PİŞİRİCİLER R., KURU B., NOYAN Ü.Publication Open Access Effects of Exercise and Calorie Restriction on Brain_x000D_ and Testis in Natural Aging Model(2021-04-25) YARAT, AYŞEN; Umay HAKGÜDER;Hazal İPEKÇİ;Tuğba TUNALI AKBAY;Ayşen YARAT;Ebru Emekli ALTURFAN;Ünsal Veli ÜSTÜNDAĞ;Burçin ALEV;Nevin GENC KAHRAMAN;Reyhan ÖZÇELİK;Göksel ŞENERThe aim of our study was to examine the effects of exercise and calorie_x000D_ restriction on various tissue damage and antioxidant parameters_x000D_ in the brain and testis of rats in a natural aging model. For this_x000D_ purpose, male Sprague-Dawley rats were the control group (C),_x000D_ the elderly (A), the elderly with calorie restriction (ACR), the elderly_x000D_ who were exercised (AE) and the elderly who were exercised with_x000D_ calorie restriction (ACRE), they were divided into 5 groups. The control_x000D_ group was composed of three-month-old animals. The other_x000D_ group consisted of 15-month-old rats. Exercise and calorie restriction_x000D_ were applied for 6 weeks. At the end of the experiment, lipid_x000D_ peroxidation (LPO), nitric oxide (NO), glutathione (GSH) levels and_x000D_ superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase_x000D_ (GST) and tissue factor (TF) were determined in brain and_x000D_ testicular tissues homogenates. As a result of the study, the A_x000D_ group’s brain and testis LPO, NO levels and TF activity increased,_x000D_ GSH levels and SOD, CAT and GST activities decreased, when compared_x000D_ to the C group. As a result of our study, an increase in oxidant_x000D_ damage was observed with TF activity in the brain and testis in the_x000D_ natural aging model, and positive effects of exercise and calorie_x000D_ restriction on the antioxidant levels in the brain were determined,_x000D_ especially in aging.Publication Metadata only Peanut (Arachis hypogaea) consumption improves glutathione and HDL-cholesterol levels in experimental diabetes(WILEY, 2008) YARAT, AYŞEN; Emekli-Alturfan, Ebru; Kasikci, Emel; Yarat, AysenThe aim of this investigation was to assess the influence of peanut (Arachis hypogaea) consumption on oxidant-antioxidant status and lipid profile in Streptozotocin (STZ) induced diabetic rats. 32 rats were divided into 4 groups as control, control+peanut, diabetic, diabetic+peanut. Control and diabetic groups were fed on standard rat chow whereas control+peanut and diabetic+peanut were fed on standard rat chow supplemented with 0.63 g % peanut for 12 weeks. Serum glucose levels, lipids, Glutathione (GSH), lipid peroxidation (LPO) and atherogenic index (AI) levels were determined at the end of the experiment. In the diabetic group TG (Triglyceride), TC (Total cholesterol), LDL-C (LDL-cholesterol) levels and atherogenic indexes increased significantly whereas HDL-C (HDL-cholesterol) level decreased significantly compared to the control group. The supplementation with peanut in the diabetic group led to significantly higher HDL-C levels and lower AI levels compared to diabetic group. Peanut consumption increased GSH levels significantly both in control and diabetic groups. In conclusion, this study shows that peanut consumption may improve oxidant-antioxidant status in healthy and diabetic status without increasing blood lipids. Moreover, increased HDL-C levels and decreased AI levels in diabetic rats indicate that, peanut consumption may have protective effects against cardiovascular complications of diabetes. Copyright (C) 2007 John Wiley & Sons, Ltd.Publication Open Access Effect of Sample Storage on Stability of Salivary Glutathione, Lipid Peroxidation Levels, and Tissue Factor Activity(WILEY, 2009) YARAT, AYŞEN; Emekli-Alturfan, Ebru; Kasikci, Emel; Alturfan, A. Ata; Pisiriciler, Rabia; Yarat, AysenSaliva samples are often required to be stored for longer periods of time either because of the project protocol or because of lack of funding for analysis. The effects of 6 months storage (fresh, 30, 60, 90 120, 150, and 180d) on the stability of salivary reduced glutathione (GSH), lipid peroxidation (LPO) and 90days of storage (fresh, 15, 30, 60, and 90d) on the stability of salivary tissue factor (TF) activity and the stability of saliva imprint samples at 20 C were evaluated in this study. Salivary GSH, malondialdehyde (MDA) levels as an index of LPO, and TF activities were determined using the methods of Beutler, Yagi, and Quick, respectively. Saliva imprint samples were stained with Giemsa and microscopically examined. Salivary GSH levels and TF MDA levels increased significantly after 6 months of storage at -20 C. Leucocyte, epithelium and bacterium cell counts did not significantly change at the end of 90 d of storage. Saliva samples may be stored up to 1 month at -20 C for LPO assay. For cytological examinations, saliva samples may be stored for 90 d at -20 C. Further studies are needed to determine the stability of salivary GSH, and salivary TF activity stored less than 30 days at -20 C. On the other hand, if saliva samples are required to be stored, to avoid the changes because of different storage periods, we recommend that they must be stored under the same circumstances and in the same time period. J. Clin. Lab. Anal. 23:93-98, 2009. (C) 2009 Wiley-Liss, Inc.Publication Metadata only Edaravone ameliorates the adverse effects of valproic acid toxicity in small intestine(SAGE PUBLICATIONS LTD, 2015) YARAT, AYŞEN; Oktay, S.; Alev, B.; Tunali, S.; Emekli-Alturfan, E.; Tunali-Akbay, T.; Koc-Ozturk, L.; Yanardag, R.; Yarat, A.Valproic acid (VPA) is a drug used for the treatment of epilepsy, bipolar psychiatric disorders, and migraine. Previous studies have reported an increased generation of reactive oxygen species and oxidative stress in the toxic mechanism of VPA. Edaravone, a free radical scavenger for clinical use, can quench free radical reaction by trapping a variety of free radical species. In this study, effect of edaravone on some small intestine biochemical parameters in VPA-induced toxicity was investigated. Thirty seven Sprague Dawley female rats were randomly divided into four groups. The groups include control group, edaravone (30 mg(-1) kg(-1) day(-1)) given group, VPA (0.5 g(-1) kg(-1) day(-1)) given group, VPA + edaravone (in same dose) given group. Edaravone and VPA were given intraperitoneally for 7 days. Biochemical parameters such as malondialdehyde, as an index of lipid peroxidation(LPO), sialic acid (SA), glutathione levels and glutathione peroxidase, glutathione-S-transferase, superoxide dismutase, catalase, myeloperoxidase, alkaline phosphatase (ALP), and tissue factor (TF) activities were determined in small intestine samples by colorimetric methods. Decreased small intestine antioxidant enzyme activities, increased LPO and SA levels, and increased activities of ALP and TF were detected in the VPA group. Based on our results edaravone may be suggested to reverse the oxidative stress and inflammation due to VPA-induced small intestine toxicity.Publication Open Access Investigation of the Effects of Edaravone on Valproic Acid Induced Tissue Damage in Pancreas(MARMARA UNIV, FAC PHARMACY, 2017-06-20) YARAT, AYŞEN; Oktay, Sehkar; Alev-Tuzuner, Burcin; Tunali, Sevim; Ak, Esin; Emekli-Alturfan, Ebru; Tunali-Akbay, Tugba; Koc-Ozturk, Leyla; Cetinel, Sule; Yanardag, Refiye; Yarat, AysenValproic acid (VPA), an effective antiepileptic and anticonvulsant drug, has some toxic side effects due to causing elevated oxidant production. The aim of this study is to investigate the effects of edaravone, a potent free radical scavenger on VPA induced toxicity and tissue damage by biochemical and histological examinations on pancreas. Female Sprague Dawley rats were divided into four groups as follows; control, edaravone, VPA, VPA+edaravon. VPA and edaravone were injected intraperitonally for seven days. Total protein, lipid peroxidation (LPO), sialic acid (SA) and glutathione (GSH) levels and alkaline phosphatase (ALP), tissue factor (TF), superoxide dismutase (SOD), glutathione-S-transferase GST), catalase (CAT), glutathione peroxidase (GPx) and myeloperoxidase (MPO) activities were determined in pancreas homogenates. In VPA given group, LPO and SA levels, and ALP, TF, MPO activities significantly increased and GST, CAT, GPx activities significantly decreased compared to control group. A marked morphological damage was detected in the VPA group. Ameliorative effects of edaravone were observed in SA, TF, CAT, GPx parameters and histological examination in the VPA group. Therefore, edaravone may be effective in moderation and/or reduction of toxic effects of VPA on pancreas.Publication Metadata only Salivary Total Sialic Acid Levels Increase in Breast Cancer Patients: A Preliminary Study(BENTHAM SCIENCE PUBL LTD, 2011) YARAT, AYŞEN; Ozturk, Leyla Koc; Emekli-Alturfan, Ebru; Kasikci, Emel; Demir, Gokhan; Yarat, AysenBreast cancer is the most common cancer in women living in the Western world, even though it occurs worldwide. Cancer and cancer therapy induce multiple oral complications including dental and periodontal disease. Saliva is a complex and dynamic biologic fluid, which reflects both oral and systemic changes. While saliva is an easily accessible body fluid, there has been little effort to study its value in cancer diagnosis. Sialic acids (SA), the end moieties of the carbohydrate chains, are biologically important and essential for functions of glycoconjugates that are reported to be altered in both blood and saliva of various cancer patients. Increased sialylation has been shown to be a characteristic feature in cancer tissue and blood in breast cancer patients. However, there is no data about salivary SA in breast cancer. The aim of this study was to evaluate salivary total sialic acid (TSA) levels in breast cancer patients who were under chemotheraphy. The study included 15 breast cancer patients in different stages and 10 healthy individuals as age-matched controls. Un-stimulated whole saliva was collected. Salivary total protein and SA levels were determined. Flow rate was calculated from salivary volume by the time of secretion. Salivary SA was significantly higher and total protein was lower in breast cancer patients compared to controls. It is concluded that sialylation may be increased in saliva of patients with breast cancer as the same way for cancer tissue and for blood. Increased salivary SA may therefore be useful as a non-invasive predictive marker for breast cancer patients and for the prevention and management of oral complications of cancer and cancer therapy to improve oral function and quality-of-life. The effects of different types of chemotherapies and different stages of the disease on salivary SA levels and salivary sialo-glycomic are worthy of being further investigated in breast cancer patients.Publication Open Access Chard extract increased gastric sialic acid and ameliorated oxidative stress in valproic acid-administered rats(2023-04-01) ALTURFAN, EBRU IŞIK; AKBAY, TUĞBA; YARAT, AYŞEN; ALEV B., TUNALI S., ÜSTÜNDAĞ Ü. V., İPEKÇİ H., ALTURFAN E. E., AKBAY T. T., YANARDAG R., YARAT A.Valproic acid, one of the most used drugs for epilepsy patients, has some known side effects. Chard extract has many pharmacological activities. The study aims to evaluate whether valproic acid might interfere with oxidative metabolism in gastric tissue and whether chard ameliorates these effects. The Sprague Dawley rats were divided into four groups (n=8); control, chard-given control, valproic acid, and chard-given valproic acid. The aqueous extracts of chard leaves were given 1 h before the administration of valproic acid for 7 days. On the 8th day, the animals were sacrificed under anesthesia and gastric tissues were homogenized. When compared to the control group, valproic acid significantly increased malondialdehyde and catalase activity, while superoxide dis- mutase activity decreased. Chard administration increased glutathione and, sialic acid levels and decreased malondialdehyde levels and superoxide dismutase activity in the valproic acid group. Based on these findings, since chard increased gastric sialic acid levels, we may suggest that chard may protect gastric mucosa may be through its antioxidant effects.Publication Metadata only Cytological investigation of saliva samples of disabled children(2013-07-11) ALTURFAN, EBRU IŞIK; AK, ESİN; YARAT, AYŞEN; PİŞİRİCİLER R., ALTURFAN E. I., OZBAY G., AK E., AKYÜZ S. H., YARAT A.Publication Metadata only Tissue factor activities of streptozotocin induced diabetic rat tissues and the effect of peanut consumption(WILEY, 2007) YARAT, AYŞEN; Emekli-Alturfan, Ebru; Kasikci, Emel; Yarat, AysenBackground Tissue factor (TF) is considered to be a major regulator of normal haemostasis and thrombosis. Circulating TF activity is suggested to be associated with diabetes mellitus. Various tissues and body fluids have TF activity. The aim of the present study was to investigate the TF activity of streptozotocin (STZ) induced diabetic rat tissues. Peanut consumption is reported to be associated with decreased risk of type 2 diabetes. Therefore, the effect of peanut consumption on the TF activity of STZ induced diabetic rat tissues, and haemostatic parameters such as protrombin time (PT), activated partial thromboplastin time (APTT) and fibrinogen levels were determined. Methods Twenty-four Wistar rats were divided into 3 groups of 8 rats each as control, STZ-induced diabetic and diabetic + peanut group. Twelve weeks later, TF activity of liver, kidney, spleen, heart, kidney, lung, pancreas and aorta and haemostatic parameters were determined. Results In the diabetic group, TF activities of liver, kidney and spleen increased (p < 0.01) whereas the TF activity of brain decreased (p < 0.01) compared to the control group. Peanut consumption in the diabetic group decreased the TF activity of spleen and aorta (p < 0.01; p < 0.05). Haemostatic parameters did not change significantly in the groups. Conclusion Elevated TF activity in diabetic rat tissues, may contribute to the increased risk of atherothrombotic disease that accompanies the diabetic complications whereas the decreased brain TF activity may be due to a different haemostatic mechanism to protect this vital organ from the diabetic status. The decreased TF activity of peanut given diabetic rat tissues might protect these tissues from the risk of thrombosis. Copyright (c) 2007 John Wiley & Sons, Ltd.
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