Person: AKSOY, HALİL
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AKSOY
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HALİL
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Publication Metadata only Erdosteine prevents colonic inflammation through its antioxidant and free radical scavenging activities(SPRINGER, 2007) AKSOY, HALİL; Sener, Goeksel; Aksoy, Halil; Sehirli, Oezer; Yueksel, Meral; Aral, Cenk; Gedik, Nursal; Cetinel, Sule; Yegen, Berrak C.After intracolonic administration of trinitrobenzene sulphonic acid (TNBS), Sprague-Dawley rats were treated orally either with saline or erdosteine (100 mg/kg per day), a sulfhydryl-containing antioxidant, for 3 days. On the 4th day, rats were decapitated and distal colon was removed for the macroscopic and microscopic damage scoring, for the measurement of malondialdehyde (MDA), glutathione (GSH) and collagen levels, myeloperoxidase (MPO) activity, luminol and lucigenin chemiluminescences (CL) and DNA fragmentation. Lactate dehydrogenase (LDH) activity, tumor necrosis factor-alpha, interleukin (IL)-1 beta, IL-6, and antioxidant capacity were assayed in blood samples. Colitis caused significant increases in the colonic CL values, macroscopic and microscopic damage scores, MDA and collagen levels, MPO activity and DNA fragmentation, along with a significant decrease in tissue GSH level. Similarly, serum cytokines and LDH were elevated in the saline-treated colitis group as compared with the control group. On the other hand, erdosteine treatment reversed all these biochemical indices, and histopathologic alterations induced by TNBS, suggesting that erdosteine protects the colonic tissue via its radical scavenging and antioxidant activities.Publication Open Access Ethanol extract of Cotinus coggygria leaves accelerates wound healing process in diabetic rats(TAYLOR & FRANCIS LTD, 2016-11) ŞEN, ALİ; Aksoy, Halil; Sen, Ali; Sancar, Mesut; Sekerler, Turgut; Akakin, Dilek; Bitis, Leyla; Uras, Fikriye; Kultur, Sukran; Izzettin, Fikret VehbiContext:Cotinus coggygria Scop. (Anacardiaceae) leaves that were used as wound healing in traditional Balkan and Anatolian folk medicine, could be potentially effective in treating diabetic wounds.Objective: This study investigates biochemical and histological effects of ethanol extract of C. coggygria (CCE) on excision wound model in diabetic rats.Materials and methods: This study was conducted on diabetic Wistar albino rats, which were injected by a single dose (50mg/kg i.p.) streptozotocin. Afterward an excision wound model was created in all animals; diabetic control rats were applied topically simple ointment and diabetic treatment rats were applied topically 5% (w/w) ointment with CC, once a day during the experimental period. Malondialdehyde, glutathione and hydroxyproline levels in wound tissues were investigated at the end of 3rd, 7th, and 14th days. Histopathological examination was also performed.Results: Hydroxyproline content was significantly increased in the CCE treated group versus control after the 3rd and 7th days (15.33 versus 11.83; 19.67 versus 15.67mg/g, p<0.05; respectively). A statistically significant elevation in glutathione at the end of 3rd, 7th, and 14th days (5.13 versus 1.58, p<0.05; 4.72 versus 1.88, p<0.05; 3.83 versus 1.88mol/g, p<0.05, respectively) and a statistically significant decrease in malondialdehyde level at the end of 7th day (4.49 versus 1.48nmol/g, p<0.05) were determined in the treated group versus control group. These results were also supported by histological analyses.Discussion and conclusion: These findings indicate that CCE accelerated the cutaneous wound healing process in diabetic wounds, in confirmation of its traditional use.Publication Metadata only Effect of Horse-chestnut seed extract on matrix metalloproteinase-1 and-9 during diabetic wound healing(WILEY, 2019) ŞEN, ALİ; Aksoy, Halil; Cevik, Ozge; Sen, Ali; Goger, Fatih; Sekerler, Turgut; Sener, AzizeThe effects of aqueous-ethanol extract of Horse chestnut (HCE) on MMP-1 and MMP-9 expressions during cutaneous wound healing in diabetic rats were investigated in this study. The expressions of MMP-1 and MMP-9, wound closure, myeloperoxidase (MPO) activity, hydroxyproline, and malondialdehyde (MDA) levels in wound tissue were measured. Quercetin glucuronide in HCE was identified as main compound using a LC-MS/MS. The hydroxyproline level was significantly increased in the treated group versus control after the 3rd and 7th days (p < 0.05). The MDA level and MPO activity were significantly lower in the treatment group (p < 0.05). MMP-1 gene expression level in treated rats was increased in the 7th day while it was reduced in 14th day. MMP-9 gene expression level in treated rats was decreased in 7th, and 14th days compared to control (p < 0.05). These results show that HCE accelerated the cutaneous wound-healing process in diabetic rats via MMP-1 and MMP-9 regulation.Publication Metadata only Evaluation of biochemical parameters inRubus tereticaulistreated rats and its implications in wound healing(SPRINGER, 2020) ŞEN, ALİ; Aksoy, Halil; Demirbag, Caglar; Sen, Ali; Sekerler, Turgut; Ozakpinar, Ozlem; Sener, Azize; Ahmad, Sarfraz; Tetik, SerminWe evaluated the effects ofRubus tereticaulisin healing process by determining the total carbonyl content, collagen synthesis, and total protein level on rat wounded tissues. Wounds were performed in the back of 54 Wistar rats, using a biopsy punch instrument with 0.6 mm in diameter. Rats were randomly divided into three groups: (i) un-treatment wounds group served as controls, (ii) Madecassol (R) used as positive control group, and (iii) the application of topical cream ofR. tereticaulisserved as treatment group of wound healing. The animals were killed at the end of experiment under anesthesia with ketamine, and tissue samples were collected for the evaluation at three times intervals (3rd, 7th, and 14th day). The wounded areas were analyzed for total carbonyl content, collagen, and total protein levels by HPLC, ELISA, and spectrophotometric methods, respectively. Total carbonyl content in the treatment group was significantly lower in comparison with control group on 3rd day (2.839 +/- 0.438 vs. 3.216 +/- 0.216 nmol carbonyl/mol protein;p < 0.5) and 14th days (4.222 +/- 0.128 vs. 4.784 +/- 0.077 nmol carbonyl/mol protein;p < 0.05), respectively. New collagen formation on the wound sites after the initial injury was noted in the treated and positive control groups (5.310 +/- 0.331 vs. 5.164 +/- 0.377 mg collagen/g wet tissue) at the 3rd day than control group (2.180 +/- 0.718 mg collagen/g wet tissue,p < 0.01), and in treated and positive control groups at 7th day (9.654 +/- 0.201, 9.053 +/- 1.062 mg collagen/g wet tissue,p < 0.01); and in treated and positive control groups at 14th day (8.469 +/- 0.236, 5.631 +/- 0.531 mg collagen/g wet tissue, respectively;p < 0.05) in comparison with the control group. Total protein level of samples did not change significantly between the groups. Thus, application ofR. tereticaulisameliorated the wound healing process in rats as it facilitated collagen formation through healing of the wound. Evaluating total carbonyl content by HPLC could be useful as an advance procedure for quantification of healing.Publication Open Access The Effect of Algan Hemostatic Agent (AHA) on Wound Healing(MARMARA UNIV, INST HEALTH SCIENCES, 2020-09-04) ŞEN, ALİ; Aksoy, Halil; Sener, Azize; Akakin, Dilek; Sen, Ali; Ozakpinar, Ozlem Bingol; Ozcan, Sinemcan; Simsek, Ahmet Kaan; Sekerler, Turgut; Guzel, Sevket Ergun; Midi, AhmetObjective: The Algan Hemostatic Agent (AHA) is a novel herbal originated blood stopper. The aim of this study is to investigate the effect of AHA on wound healing on excisional wound model in rats. Methods: In this study, 54 adult Wistar albino rats were used. Rats were divided into 3 groups (saline, Madecassol (R) and AHA). Each group was then divided into 3 subgroups as the 3rd, 7th and 14th days. Two wounds were created in the dorsal thoracic region of the rats. One of the lesions was used for histopathological examinations and the other for hydroxyproline measurement. In order to evaluate the wound healing, wound area were measured during the whole treatment period and animals were sacrificed at the end of the 3rd, 7th and 14th days and tissue samples were taken for the determination of hydroxyproline levels. Results: AHA treatment did not cause significant difference in hydroxyproline level on days 3, 7, 14. The contraction percentage of wound area was higher in the AHA group on day 7 than that of the control group. However, the difference was not statistically significant (p>0.05). On days 3 and 14, no significant difference was detected in the contraction percentage of wound area between the control and the AHA groups. AHA and Madecassol (R) results of epidermis regeneration on the 14th day, neutrophil infiltration on the 7th day and edema on the 3rd, 7th and 14th days were different in terms of histopathological parameters compared to the control group. Conclusion: Despite good histological findings, AHA did not significantly accelerate wound healing, but did not adversely affect wound healing as well.Publication Metadata only Chemometric approach to simultaneous chromatographic determination of paracetamol and chlorzoxazone in tablets and spiked human plasma(TAYLOR & FRANCIS INC, 2006) AKSOY, HALİL; Dinc, E; Ozdemir, A; Aksoy, H; Baleanu, DNew chemometric approaches were introduced into the high performance liquid chromatographic (HPLC) determination of chlorzoxazone (CZX) and paracetamol (PAR) in the tablets and spiked human plasma. These chemometric approaches contain the application of classical least squares (CLS), principle component regression (PCR), and partial least squares (PLS) calibrations to the multiwavelength HPLC peak area obtained by plotting the chromatograms at the five wavelengths. The multichromatograms were obtained by using the photodiode array detector at 225 (A), 240 (B), 255 (C), 270 (D), and 285 (E) nm. The algorithms of CLS, PCR, and PLS were applied to the multichromatogram data to construct the HPLC-CLS, HPLC-PCR, and HPLC-PLS calibrations. A mixture of acetonitrile and 0.1 M ammonium carbonate (60:40, v/v) on a Waters Symmetry (R) trademark C18 Column 5 mu m 4.6 x 250 mm at a flow rate of 0.8 mL/min was used as a mobile phase to separate and determine CZX and PAR in samples. Hydrochlorothiazide (IS) was used as an internal standard in this chromatographic separation. The HPLC chemometric calibrations were successfully applied to the quantitative analysis of the investigated drugs in commercial pharmaceutical preparation and spiked human plasma samples with high precision and accuracy.Publication Metadata only The effect of topical ethanol extract of Cotinus coggygria Scop. on cutaneous wound healing in rats(TAYLOR & FRANCIS LTD, 2016) ŞEN, ALİ; Aksoy, Halil; Sancar, Mesut; Sen, Ali; Okuyan, Betul; Bitis, Leyla; Uras, Fikriye; Akakin, Dilek; Cevik, Ozge; Kultur, Sukran; Izzettin, Fikret VehbiThe aim of this study is to determine the cutaneous wound healing effects of the ethanol extract of Cotinus coggygria leaves in rats by excision wound model to provide scientific evidence for the traditional use of C. coggygria Scop. The levels of malondialdehyde, catalase, superoxide dismutase, glutathione and hydroxyproline were investigated in wound tissues. Histopathological examination was also performed. The hydroxyproline content of the granulation tissue and the glutathione levels were both significantly higher in the treatment group than in the control group (p < 0.05 for both); while the malondialdehyde levels were significantly lower in the treatment group (p < 0.05). These results were supported with histological results. The ethanol extract of C. coggygria Scop could be considered as an effective agent in wound healing in accordance with its traditional use.Publication Open Access Evaluation of the Biological and Wound Healing Activities of Centaurea virgata Lam.(MARMARA UNIV, INST HEALTH SCIENCES, 2020-09-10) AKSOY, HALİL; Aksoy, HalilObjective: Plants have significant potential effects in treating wounds. Some plants are also used in traditional medicine for their wound healing properties. The aim of this study was to investigate the antioxidant and in vitro wound healing activities of Centaurea virgata. Methods: The antioxidant activities ofextracts were examined by 2,2-diphenyl-1-picryl-hydrazyl (DPPH) and 2,2-azinobis-(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) methods. The total phenolic content was determined using the Folin-Ciocalteu reagent (FCR) method. In addition, the antiproliferative activity of different extracts on fibroblast cells was performed by MTT method and in vitro wound healing activity of ethanol extract of C. virgata was determined by scratch assay. Results: Ethyl acetate fraction of ethanol extract of C. virgata (CVEA) had the highest DPPH radical scavenging activity with an IC50 value of 138.7 mu g/mL. According to ABTS results, it was determined that the CVEA had a stronger radical scavenging activity than the other extracts. Hexane (CVH) and aqueous ethanol fractions (CVAE) revealed poor antioxidant activity with IC50 values of 824.8 mu g/mL and 610.3 mu g/mL, respectively. On the other hand, among all of the extracts analysed, it was found that phenolic content of CVEA was higher than other extracts. Except for CVAE, other extracts showed antiproliferative activity depending on the dose. Conclusion: According to the obtained results, our finding suggests that CVAE has a migratory effect on fibroblasts and that CVAE might be a potential therapeutic agent for wound healing.Publication Open Access Yara İyileşmesi ve Oksidatif Stress(2014-09-08) AKSOY, HALİLPublication Metadata only The biochemical assessment of the effectsof Nigella sativa oil on cutaneous wound healing in rats(2010-06-09) RABUŞ, ŞULE; SANCAR, MESUT; OKUYAN, BETÜL; AKSOY, HALİL; AKSOY H. Z., RABUŞ Ş., SANCAR M., OKUYAN B., URAS F., İZZETTİN F. V.