Person: AKSOY, HALİL
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AKSOY
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HALİL
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Publication Open Access Ethanol extract of Cotinus coggygria leaves accelerates wound healing process in diabetic rats(TAYLOR & FRANCIS LTD, 2016-11) ŞEN, ALİ; Aksoy, Halil; Sen, Ali; Sancar, Mesut; Sekerler, Turgut; Akakin, Dilek; Bitis, Leyla; Uras, Fikriye; Kultur, Sukran; Izzettin, Fikret VehbiContext:Cotinus coggygria Scop. (Anacardiaceae) leaves that were used as wound healing in traditional Balkan and Anatolian folk medicine, could be potentially effective in treating diabetic wounds.Objective: This study investigates biochemical and histological effects of ethanol extract of C. coggygria (CCE) on excision wound model in diabetic rats.Materials and methods: This study was conducted on diabetic Wistar albino rats, which were injected by a single dose (50mg/kg i.p.) streptozotocin. Afterward an excision wound model was created in all animals; diabetic control rats were applied topically simple ointment and diabetic treatment rats were applied topically 5% (w/w) ointment with CC, once a day during the experimental period. Malondialdehyde, glutathione and hydroxyproline levels in wound tissues were investigated at the end of 3rd, 7th, and 14th days. Histopathological examination was also performed.Results: Hydroxyproline content was significantly increased in the CCE treated group versus control after the 3rd and 7th days (15.33 versus 11.83; 19.67 versus 15.67mg/g, p<0.05; respectively). A statistically significant elevation in glutathione at the end of 3rd, 7th, and 14th days (5.13 versus 1.58, p<0.05; 4.72 versus 1.88, p<0.05; 3.83 versus 1.88mol/g, p<0.05, respectively) and a statistically significant decrease in malondialdehyde level at the end of 7th day (4.49 versus 1.48nmol/g, p<0.05) were determined in the treated group versus control group. These results were also supported by histological analyses.Discussion and conclusion: These findings indicate that CCE accelerated the cutaneous wound healing process in diabetic wounds, in confirmation of its traditional use.Publication Open Access The Effect of Algan Hemostatic Agent (AHA) on Wound Healing(MARMARA UNIV, INST HEALTH SCIENCES, 2020-09-04) ŞEN, ALİ; Aksoy, Halil; Sener, Azize; Akakin, Dilek; Sen, Ali; Ozakpinar, Ozlem Bingol; Ozcan, Sinemcan; Simsek, Ahmet Kaan; Sekerler, Turgut; Guzel, Sevket Ergun; Midi, AhmetObjective: The Algan Hemostatic Agent (AHA) is a novel herbal originated blood stopper. The aim of this study is to investigate the effect of AHA on wound healing on excisional wound model in rats. Methods: In this study, 54 adult Wistar albino rats were used. Rats were divided into 3 groups (saline, Madecassol (R) and AHA). Each group was then divided into 3 subgroups as the 3rd, 7th and 14th days. Two wounds were created in the dorsal thoracic region of the rats. One of the lesions was used for histopathological examinations and the other for hydroxyproline measurement. In order to evaluate the wound healing, wound area were measured during the whole treatment period and animals were sacrificed at the end of the 3rd, 7th and 14th days and tissue samples were taken for the determination of hydroxyproline levels. Results: AHA treatment did not cause significant difference in hydroxyproline level on days 3, 7, 14. The contraction percentage of wound area was higher in the AHA group on day 7 than that of the control group. However, the difference was not statistically significant (p>0.05). On days 3 and 14, no significant difference was detected in the contraction percentage of wound area between the control and the AHA groups. AHA and Madecassol (R) results of epidermis regeneration on the 14th day, neutrophil infiltration on the 7th day and edema on the 3rd, 7th and 14th days were different in terms of histopathological parameters compared to the control group. Conclusion: Despite good histological findings, AHA did not significantly accelerate wound healing, but did not adversely affect wound healing as well.Publication Open Access Evaluation of the Biological and Wound Healing Activities of Centaurea virgata Lam.(MARMARA UNIV, INST HEALTH SCIENCES, 2020-09-10) AKSOY, HALİL; Aksoy, HalilObjective: Plants have significant potential effects in treating wounds. Some plants are also used in traditional medicine for their wound healing properties. The aim of this study was to investigate the antioxidant and in vitro wound healing activities of Centaurea virgata. Methods: The antioxidant activities ofextracts were examined by 2,2-diphenyl-1-picryl-hydrazyl (DPPH) and 2,2-azinobis-(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) methods. The total phenolic content was determined using the Folin-Ciocalteu reagent (FCR) method. In addition, the antiproliferative activity of different extracts on fibroblast cells was performed by MTT method and in vitro wound healing activity of ethanol extract of C. virgata was determined by scratch assay. Results: Ethyl acetate fraction of ethanol extract of C. virgata (CVEA) had the highest DPPH radical scavenging activity with an IC50 value of 138.7 mu g/mL. According to ABTS results, it was determined that the CVEA had a stronger radical scavenging activity than the other extracts. Hexane (CVH) and aqueous ethanol fractions (CVAE) revealed poor antioxidant activity with IC50 values of 824.8 mu g/mL and 610.3 mu g/mL, respectively. On the other hand, among all of the extracts analysed, it was found that phenolic content of CVEA was higher than other extracts. Except for CVAE, other extracts showed antiproliferative activity depending on the dose. Conclusion: According to the obtained results, our finding suggests that CVAE has a migratory effect on fibroblasts and that CVAE might be a potential therapeutic agent for wound healing.Publication Open Access Yara İyileşmesi ve Oksidatif Stress(2014-09-08) AKSOY, HALİLPublication Open Access In silico investigation of wound healing potential of some compounds in tubers of Asphodelus species with GSK3-beta protein(MARMARA UNIV, 2021) AKSOY, HALİL; Aksoy, Halil; Sekerler, Turgut; Mert, Naz MinaWound healing is a process that involves biochemical processes such as inflammation and cell proliferation and is controlled by many proteins. It is known that one of the most effective factors in this process is the inhibition of GSK3-beta protein. In current study, in silico wound healing activity of the some compounds found in the tubers of Asphodelus species used as a wound healing in traditional medicine were investigated. For this purpose, the interactions between the compounds and GSK3-beta protein were studied in silico. As a result of the study, it has been determined that and stigmasterol, beta-sitosterol and emodin molecules are effective.